JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goodwin, R. L.
Right arrow Articles by Bader, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goodwin, R. L.
Right arrow Articles by Bader, D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 274, Issue 26, 18597-18604, June 25, 1999

The Cloning and Analysis of LEK1 Identifies Variations in the LEK/Centromere Protein F/Mitosin Gene Family

Richard L. Goodwin, Lil M. Pabón-Peña, Gayle C. Foster, and David Bader

From the Gladys P. Stahlman Cardiovascular Research Laboratory, Vanderbilt University Medical Center, Nashville, Tennessee 37212-6300

We report the cloning of a novel murine cDNA, LEK1, that is related to human CENP-F and mitosin and more distantly to chicken CMF1. The proteins from these three organisms have significant homology, yet differ in their temporal, spatial, and subcellular localizations. The human proteins bind the kinetochore in mitotic cells, whereas the chicken protein is found only in skeletal and cardiac muscle and is developmentally regulated. Mouse LEK1 is a single copy gene that codes for two developmentally regulated transcripts. The LEK1 protein is expressed early and ubiquitously in mouse development and is generally down-regulated as development proceeds in a manner that correlates to a cessation of mitosis. In adult tissues, the LEK1 protein is detected exclusively in the pronucleus of the oocyte and was not observed in other actively dividing tissues. Subcellular localization revealed that the LEK1 protein in mitotic cells does not bind the kinetochore. From these data, we hypothesize that chicken CMF1, human CENP-F, mitosin, and mouse LEK1 are members of an emerging family of genes that have important and functionally distinct roles in development and cell division.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
R. D. Pooley, K. L. Moynihan, V. Soukoulis, S. Reddy, R. Francis, C. Lo, L.-J. Ma, and D. M. Bader
Murine CENPF interacts with syntaxin 4 in the regulation of vesicular transport
J. Cell Sci., October 15, 2008; 121(20): 3413 - 3421.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Q. He, F. O. Eko, D. Lyn, G. A. Ananaba, C. Bandea, J. Martinez, K. Joseph, K. Kellar, C. M. Black, and J. U. Igietseme
Involvement of LEK1 in Dendritic Cell Regulation of T Cell Immunity against Chlamydia
J. Immunol., September 15, 2008; 181(6): 4037 - 4042.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. D. Pooley, S. Reddy, V. Soukoulis, J. T. Roland, J. R. Goldenring, and D. M. Bader
CytLEK1 Is a Regulator of Plasma Membrane Recycling through Its Interaction with SNAP-25
Mol. Biol. Cell, July 1, 2006; 17(7): 3176 - 3186.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. V. Holt, M. A. S. Vergnolle, D. Hussein, M. J. Wozniak, V. J. Allan, and S. S. Taylor
Silencing Cenp-F weakens centromeric cohesion, prevents chromosome alignment and activates the spindle checkpoint
J. Cell Sci., October 15, 2005; 118(20): 4889 - 4900.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
V. Soukoulis, S. Reddy, R. D. Pooley, Y. Feng, C. A. Walsh, and D. M. Bader
Cytoplasmic LEK1 is a regulator of microtubule function through its interaction with the LIS1 pathway
PNAS, June 14, 2005; 102(24): 8549 - 8554.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Zhou, R. Wang, L. Fan, Y. Li, L. Ma, Z. Yang, W. Yu, N. Jing, and X. Zhu
Mitosin/CENP-F as a Negative Regulator of Activating Transcription Factor-4
J. Biol. Chem., April 8, 2005; 280(14): 13973 - 13977.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Ashe, L. Pabon-Pena, E. Dees, K. L. Price, and D. Bader
LEK1 Is a Potential Inhibitor of Pocket Protein-mediated Cellular Processes
J. Biol. Chem., January 2, 2004; 279(1): 664 - 676.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. M. Wada, D. E. Reese, and D. M. Bader
Bves: prototype of a new class of cell adhesion molecules expressed during coronary artery development
Development, June 1, 2001; 128(11): 2085 - 2093.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. M. Pabon-Pena, R. L. Goodwin, L. J. Cise, and D. Bader
Analysis of CMF1 Reveals a Bone Morphogenetic Protein-independent Component of the Cardiomyogenic Pathway
J. Biol. Chem., July 7, 2000; 275(28): 21453 - 21459.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.