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J Biol Chem, Vol. 274, Issue 26, 18597-18604, June 25, 1999
The Cloning and Analysis of LEK1 Identifies Variations in the
LEK/Centromere Protein F/Mitosin Gene Family
Richard L.
Goodwin,
Lil M.
Pabón-Peña,
Gayle C.
Foster, and
David
Bader
From the Gladys P. Stahlman Cardiovascular Research Laboratory,
Vanderbilt University Medical Center,
Nashville, Tennessee 37212-6300
We report the cloning of a novel murine cDNA,
LEK1, that is related to human CENP-F and mitosin and more distantly to
chicken CMF1. The proteins from these three organisms have significant homology, yet differ in their temporal, spatial, and subcellular localizations. The human proteins bind the kinetochore in mitotic cells, whereas the chicken protein is found only in skeletal and cardiac muscle and is developmentally regulated. Mouse LEK1 is a single
copy gene that codes for two developmentally regulated transcripts. The
LEK1 protein is expressed early and ubiquitously in mouse development
and is generally down-regulated as development proceeds in a manner
that correlates to a cessation of mitosis. In adult tissues, the LEK1
protein is detected exclusively in the pronucleus of the oocyte and was
not observed in other actively dividing tissues. Subcellular
localization revealed that the LEK1 protein in mitotic cells does not
bind the kinetochore. From these data, we hypothesize that chicken
CMF1, human CENP-F, mitosin, and mouse LEK1 are members of an emerging
family of genes that have important and functionally distinct roles in
development and cell division.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.

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Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.
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