| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 26, 18651-18658, June 25, 1999
From the Department of Physiology and Biophysics, the University of
Iowa, Iowa City, Iowa 52242-1109
Previous studies have suggested that the
interaction between the small adaptor protein Grb2 with the Ras guanyl
nucleotide exchange factor SOS is functionally important in the
regulation of the Ras activation/inactivation cycle. To examine the
relationship between the Grb2-SOS complex and Ras activation, we
observed that insulin stimulation results in a rapid but transient
activation of Ras and the extracellular-signal regulated kinase (ERK)
followed by dissociation of the Grb2-SOS complex. Although treatment
with the phorbol myristate acetate resulted in ERK activation and
complete dissociation of the Grb2-SOS complex, there was no effect on
subsequent insulin-stimulated Ras activation. Similarly, insulin
stimulation followed by insulin removal resulted in a
time-dependent restoration of the Grb2-SOS complex but
which was significantly slower than the recovery of insulin-stimulated
Ras activation. In addition, although insulin was able to activate Ras
under these conditions, there was a complete desensitization of Raf and
ERK activation. This apparent homologous desensitization of insulin
action was specific for Raf and ERK as the insulin re-stimulation of
insulin receptor autophosphorylation and protein kinase B activation
were unaffected. Together, these data demonstrate the presence of a pathway independent of the Grb2-SOS complex that can lead to Ras activation but that the desensitization of Raf accounts for the homologous desensitization of ERK.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Lei, C. N. Mariash, M. Bhargava, E. V. Wattenberg, and D. H. Ingbar T3 increases Na-K-ATPase activity via a MAPK/ERK1/2-dependent pathway in rat adult alveolar epithelial cells Am J Physiol Lung Cell Mol Physiol, April 1, 2008; 294(4): L749 - L754. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. C. Galownia, K. Kushiro, Y. Gong, and A. R. Asthagiri Selective Desensitization of Growth Factor Signaling by Cell Adhesion to Fibronectin J. Biol. Chem., July 27, 2007; 282(30): 21758 - 21766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Hou, S. Shigematsu, H. C. Crawford, P. Z. Anastasiadis, and J. E. Pessin Dual Regulation of Rho and Rac by p120 Catenin Controls Adipocyte Plasma Membrane Trafficking J. Biol. Chem., August 18, 2006; 281(33): 23307 - 23312. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Sharma, H.-S. Son, S. Ugi, W. Ricketts, and J. M. Olefsky Mechanism of SHIP-Mediated Inhibition of Insulin- and Platelet-Derived Growth Factor-Stimulated Mitogen-Activated Protein Kinase Activity in 3T3-L1 Adipocytes Mol. Endocrinol., February 1, 2005; 19(2): 421 - 430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Hupfeld, J. L. Resnik, S. Ugi, and J. M. Olefsky Insulin-induced {beta}-Arrestin1 Ser-412 Phosphorylation Is a Mechanism for Desensitization of ERK Activation by G{alpha}i-coupled Receptors J. Biol. Chem., January 14, 2005; 280(2): 1016 - 1023. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Marrero, D. Fulton, D. Stepp, and D. M. Stern Angiotensin II-Induced Insulin Resistance and Protein Tyrosine Phosphatases Arterioscler. Thromb. Vasc. Biol., November 1, 2004; 24(11): 2009 - 2013. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Fringer and F. Grinnell Fibroblast Quiescence in Floating Collagen Matrices: DECREASE IN SERUM ACTIVATION OF MEK AND RAF BUT NOT RAS J. Biol. Chem., May 30, 2003; 278(23): 20612 - 20617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Pirola, S. Bonnafous, A. M. Johnston, C. Chaussade, F. Portis, and E. Van Obberghen Phosphoinositide 3-Kinase-mediated Reduction of Insulin Receptor Substrate-1/2 Protein Expression via Different Mechanisms Contributes to the Insulin-induced Desensitization of Its Signaling Pathways in L6 Muscle Cells J. Biol. Chem., April 25, 2003; 278(18): 15641 - 15651. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ugi, T. Imamura, W. Ricketts, and J. M. Olefsky Protein Phosphatase 2A Forms a Molecular Complex with Shc and Regulates Shc Tyrosine Phosphorylation and Downstream Mitogenic Signaling Mol. Cell. Biol., April 1, 2002; 22(7): 2375 - 2387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Le Roith and Y. Zick Recent Advances in Our Understanding of Insulin Action and Insulin Resistance Diabetes Care, March 1, 2001; 24(3): 588 - 597. [Abstract] [Full Text]
|
|
|
|
 |
|
 R.-S. Schmid, W. M. Pruitt, and P. F. Maness A MAP Kinase-Signaling Pathway Mediates Neurite Outgrowth on L1 and Requires Src-Dependent Endocytosis J. Neurosci., June 1, 2000; 20(11): 4177 - 4188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hamilton, J. Liao, M. K. Cathcart, and A. Wolfman Constitutive Association of c-N-Ras with c-Raf-1 and Protein Kinase Cepsilon in Latent Signaling Modules J. Biol. Chem., July 27, 2001; 276(31): 29079 - 29090. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
