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J Biol Chem, Vol. 274, Issue 27, 18887-18892, July 2, 1999

Adenylyl Cyclase, a Coincidence Detector for Nitric Oxide

Michael McVey, Jennifer Hill, Allyn HowlettDagger , and Claudette Klein

From the Departments of Biochemistry and Molecular Biology and Dagger  Pharmacological and Physiological Science, Saint Louis University School of Medicine, St. Louis, Missouri 63104

Nitric oxide (NO) donors inhibit hormone- and forskolin-stimulated adenylyl cyclase activity in purified plasma membrane preparations from N18TG2 neuroblastoma cells. Northern blot analyses indicate that the predominant isoform of adenylyl cyclase in N18TG2 cells is the type VI. Our experiments eliminate all the known regulatory proteins for this isoform as possible targets of NO. NO decreases the Vmax of the enzyme without altering the Km for ATP. Occupancy of the substrate-binding site protects the enzyme from the inhibitory effects of NO, suggesting that the conformation of the enzyme determines its sensitivity. The inhibition is reversed by reducing agents, implicating a Cys residue(s) as the target for nitric oxide and an S-nitrosylation as the underlying modification. These findings implicate NO as a novel cellular regulator of the type VI isoform of adenylyl cyclase.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.


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