![]()
|
|
||||||||
J Biol Chem, Vol. 274, Issue 29, 20265-20270, July 16, 1999
From the The plasma copper protein ceruloplasmin (CP) was
found to inhibit endothelial nitric-oxide synthase activation in
cultured endothelial cells, in line with previous evidence showing that the endothelium-dependent vasorelaxation of the aorta is
impaired by physiological concentrations of ceruloplasmin. The data
presented here indicate a direct relationship between the extent of
inhibition of agonist-triggered endothelial nitric oxide synthase
activation and CP-induced enrichment of the copper content of
endothelial cells. Copper discharged by CP was mainly localized in the
soluble fraction of cells. The subcellular distribution of the metal
seems to be of relevance to the inhibitory effect of CP, because it was
mimicked by copper chelates, like copper-histidine, able to selectively
enrich the cytosolic fraction of cells, but not by copper salts, which
preferentially located the metal to the particulate fraction.
Inhibition of Endothelial Nitric-oxide Synthase by
Ceruloplasmin
,
Department of Biochemical Sciences,
University La Sapienza, Piazzale Aldo Moro 5, 00185 Rome,
Italy,§ Department of Organic and Biological Chemistry,
University of Messina, Salita Sperone 31, 98166 S. Agata Messina,
Italy, ¶ Department of Biology, University Roma Tre, Viale Marconi
446, 00146 Rome, Italy, and
CNR Center of Molecular Biology,
University La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
![]()
CiteULike
Complore
Connotea
Del.icio.us
Digg
Reddit
Technorati What's this?
This article has been cited by other articles:
![]() |
E. Sedlak, G. Zoldak, and P. Wittung-Stafshede Role of Copper in Thermal Stability of Human Ceruloplasmin Biophys. J., February 15, 2008; 94(4): 1384 - 1391. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. J. Sullivan, T. H. Teal, I. P. Luttrell, K. B. Tran, M. A. Peters, and H. Wessells Microarray analysis reveals novel gene expression changes associated with erectile dysfunction in diabetic rats Physiol Genomics, October 17, 2005; 23(2): 192 - 205. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. M. Lee, H.-W. Lo, R.-P. Shao, S.-C. Wang, W. Xia, D. M. Gershenson, and M.-C. Hung Selective Activation of Ceruloplasmin Promoter in Ovarian Tumors: Potential Use for Gene Therapy Cancer Res., March 1, 2004; 64(5): 1788 - 1793. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. Bielli, G. C. Bellenchi, and L. Calabrese Site-directed Mutagenesis of Human Ceruloplasmin. PRODUCTION OF A PROTEOLYTICALLY STABLE PROTEIN AND STRUCTURE-ACTIVITY RELATIONSHIPS OF TYPE 1 SITES J. Biol. Chem., January 19, 2001; 276(4): 2678 - 2685. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |