JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aoe, T.
Right arrow Articles by Hsu, V. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aoe, T.
Right arrow Articles by Hsu, V. W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 274, Issue 29, 20545-20549, July 16, 1999

The KDEL Receptor Regulates a GTPase-activating Protein for ADP-ribosylation Factor 1 by Interacting with Its Non-catalytic Domain

Tomohiko Aoe, Irit Huber§, Chandrasekaran Vasudevan, Simon C. Watkins**, Guillermo Romero, Dan Cassel§, and Victor W. Hsu

From the Division of Rheumatology, Allergy, and Immunology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, the § Department of Biology, Technion Institute of Technology, Haifa 32000, Israel, and the  Departments of Pharmacology and Cell Biology and ** Center for Biological Imaging and Department of Cell Biology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261

ADP-ribosylation factor 1 (ARF1) is a key regulator of transport in the secretory system. Like all small GTPases, deactivation of ARF1 requires a GTPase-activating protein (GAP) that promotes hydrolysis of GTP to GDP on ARF1. Structure-function analysis of a GAP for ARF1 revealed that its activity in vivo requires not only a domain that catalyzes hydrolysis of GTP on ARF1 but also a non-catalytic domain. In this study, we show that the non-catalytic domain of GAP is required for its recruitment from cytosol to membranes and that this domain mediates the interaction of GAP with the transmembrane KDEL receptor. Blocking its interaction with the KDEL receptor leaves the GAP cytosolic and prevents the deactivation in vivo of Golgi-localized ARF1. Thus, these findings suggest that the KDEL receptor plays a critical role in the function of GAP by regulating its recruitment from cytosol to membranes, where it can then act on its membrane-restricted target, the GTP-bound form of ARF1.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
C. Schindler and A. Spang
Interaction of SNAREs with ArfGAPs Precedes Recruitment of Sec18p/NSF
Mol. Biol. Cell, August 1, 2007; 18(8): 2852 - 2863.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
N. Yahara, K. Sato, and A. Nakano
The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus
J. Cell Sci., June 15, 2006; 119(12): 2604 - 2612.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
W. Natsume, K. Tanabe, S. Kon, N. Yoshida, T. Watanabe, T. Torii, and M. Satake
SMAP2, a Novel ARF GTPase-activating Protein, Interacts with Clathrin and Clathrin Assembly Protein and Functions on the AP-1-positive Early Endosome/Trans-Golgi Network
Mol. Biol. Cell, June 1, 2006; 17(6): 2592 - 2603.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
Z. Nie and P. A. Randazzo
Arf GAPs and membrane traffic.
J. Cell Sci., April 1, 2006; 119(Pt 7): 1203 - 1211.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Parnis, M. Rawet, L. Regev, B. Barkan, M. Rotman, M. Gaitner, and D. Cassel
Golgi Localization Determinants in ArfGAP1 and in New Tissue-specific ArfGAP1 Isoforms
J. Biol. Chem., February 17, 2006; 281(7): 3785 - 3792.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
W. Liu, R. Duden, R. D. Phair, and J. Lippincott-Schwartz
ArfGAP1 dynamics and its role in COPI coat assembly on Golgi membranes of living cells
J. Cell Biol., March 28, 2005; 168(7): 1053 - 1063.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
S. L. Pfluger, H. V. Goodson, J. M. Moran, C. J. Ruggiero, X. Ye, K. M. Emmons, and K. M. Hager
Receptor for Retrograde Transport in the Apicomplexan Parasite Toxoplasma gondii
Eukaryot. Cell, February 1, 2005; 4(2): 432 - 442.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Yamamoto, H. Hamada, H. Shinkai, Y. Kohno, H. Koseki, and T. Aoe
The KDEL Receptor Modulates the Endoplasmic Reticulum Stress Response through Mitogen-activated Protein Kinase Signaling Cascades
J. Biol. Chem., September 5, 2003; 278(36): 34525 - 34532.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Reinhard, M. Schweikert, F. T. Wieland, and W. Nickel
Functional reconstitution of COPI coat assembly and disassembly using chemically defined components
PNAS, July 8, 2003; 100(14): 8253 - 8257.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. M. Jacques, Z. Nie, S. Stauffer, D. S. Hirsch, L.-X. Chen, K. T. Stanley, and P. A. Randazzo
Arf1 Dissociates from the Clathrin Adaptor GGA Prior to Being Inactivated by Arf GTPase-activating Proteins
J. Biol. Chem., November 27, 2002; 277(49): 47235 - 47241.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
J.-S. Yang, S. Y. Lee, M. Gao, S. Bourgoin, P. A. Randazzo, R. T. Premont, and V. W. Hsu
ARFGAP1 promotes the formation of COPI vesicles, suggesting function as a component of the coat
J. Cell Biol., October 14, 2002; 159(1): 69 - 78.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Szafer, E. Pick, M. Rotman, S. Zuck, I. Huber, and D. Cassel
Role of Coatomer and Phospholipids in GTPase-activating Protein-dependent Hydrolysis of GTP by ADP-ribosylation Factor-1
J. Biol. Chem., July 28, 2000; 275(31): 23615 - 23619.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.