JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zheng, X.
Right arrow Articles by Sadler, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zheng, X.
Right arrow Articles by Sadler, J. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 274, Issue 3, 1596-1605, January 15, 1999

Apical Sorting of Bovine Enteropeptidase Does Not Involve Detergent-resistant Association with Sphingolipid-Cholesterol Rafts

Xinglong Zheng, Deshun Lu, and J. Evan Sadler

From the Division of Hematology and Oncology, Department of Medicine and Barnes-Jewish Hospital, Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110

Enteropeptidase is a heterodimeric type II membrane protein of the brush border of duodenal enterocytes. In this location, enteropeptidase cleaves and activates trypsinogen, thereby initiating the activation of other intestinal digestive enzymes. Recombinant bovine enteropeptidase was sorted directly to the apical surface of polarized Madin-Darby canine kidney cells. Replacement of the cytoplasmic and signal anchor domains with a cleavable signal peptide (mutant proenteropeptidase lacking the amino-terminal signal anchor domain (dSA-BEK)) caused apical secretion. The additional amino-terminal deletion of a mucin-like domain (HL-BEK) resulted in secretion both apically and basolaterally. Further deletion of the noncatalytic heavy chain (L-BEK) resulted in apical secretion. Thus enteropeptidase appears to have at least three distinct sorting signals as follows: the light chain (L-BEK) directs apical sorting, addition of most of the heavy chain (HL-BEK) inhibits apical sorting, and addition of the mucin-like domain (dSA-BEK) restores apical sorting. Inhibition of N-linked glycosylation with tunicamycin or disruption of microtubules with colchicine caused L-BEK to be secreted equally into apical and basolateral compartments, whereas brefeldin A caused basolateral secretion of L-BEK. Full-length BEK was not found in detergent-resistant raft domains of Madin-Darby canine kidney cells or baby hamster kidney cells. These results suggest apical sorting of enteropeptidase depends on N-linked glycosylation of the serine protease domain and an amino-terminal segment that includes an O-glycosylated mucin-like domain and three potential N-glycosylation sites. In contrast to many apically targeted proteins, enteropeptidase does not form detergent-resistant associations with sphingolipid-cholesterol rafts.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
E.-J. Kamsteeg, A. S. Duffield, I. B. M. Konings, J. Spencer, P. Pagel, P. M. T. Deen, and M. J. Caplan
MAL decreases the internalization of the aquaporin-2 water channel
PNAS, October 16, 2007; 104(42): 16696 - 16701.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Liao, W. Wang, S. Chen, and Q. Wu
Role of Glycosylation in Corin Zymogen Activation
J. Biol. Chem., September 21, 2007; 282(38): 27728 - 27735.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. Garcia-Garcia, E. J. Brown, and C. Rosales
Transmembrane Mutations to Fc{gamma}RIIA Alter Its Association with Lipid Rafts: Implications for Receptor Signaling
J. Immunol., March 1, 2007; 178(5): 3048 - 3058.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. S. Chmelar and N. M. Nathanson
Identification of a Novel Apical Sorting Motif and Mechanism of Targeting of the M2 Muscarinic Acetylcholine Receptor
J. Biol. Chem., November 17, 2006; 281(46): 35381 - 35396.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
D. Shang, X. W. Zheng, M. Niiya, and X. L. Zheng
Apical sorting of ADAMTS13 in vascular endothelial cells and Madin-Darby canine kidney cells depends on the CUB domains and their association with lipid rafts
Blood, October 1, 2006; 108(7): 2207 - 2215.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Alfalah, G. Wetzel, I. Fischer, R. Busche, E. E. Sterchi, K.-P. Zimmer, H.-P. Sallmann, and H. Y. Naim
A Novel Type of Detergent-resistant Membranes May Contribute to an Early Protein Sorting Event in Epithelial Cells
J. Biol. Chem., December 30, 2005; 280(52): 42636 - 42643.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
G. Coudroy, J. Gburek, R. Kozyraki, M. Madsen, G. Trugnan, S. K. Moestrup, P. J. Verroust, and M. Maurice
Contribution of Cubilin and Amnionless to Processing and Membrane Targeting of Cubilin-Amnionless Complex
J. Am. Soc. Nephrol., August 1, 2005; 16(8): 2330 - 2337.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Jacob, M. Heine, J. Eikemeyer, N. Frerker, K.-P. Zimmer, U. Rescher, V. Gerke, and H. Y. Naim
Annexin II Is Required for Apical Transport in Polarized Epithelial Cells
J. Biol. Chem., January 30, 2004; 279(5): 3680 - 3684.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Imamura and Y. Kitamoto
Expression of enteropeptidase in differentiated enterocytes, goblet cells, and the tumor cells in human duodenum
Am J Physiol Gastrointest Liver Physiol, December 1, 2003; 285(6): G1235 - G1241.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Zheng, K. Nishio, E. M. Majerus, and J. E. Sadler
Cleavage of von Willebrand Factor Requires the Spacer Domain of the Metalloprotease ADAMTS13
J. Biol. Chem., August 8, 2003; 278(32): 30136 - 30141.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Tabuchi, N. Tanaka, J. Nishida-Kitayama, H. Ohno, and F. Kishi
Alternative Splicing Regulates the Subcellular Localization of Divalent Metal Transporter 1 Isoforms
Mol. Biol. Cell, December 1, 2002; 13(12): 4371 - 4387.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Physiol.Home page
G. Vuagniaux, V. Vallet, N. F. Jaeger, E. Hummler, and B. C. Rossier
Synergistic Activation of ENaC by Three Membrane-bound Channel-activating Serine Proteases (mCAP1, mCAP2, and mCAP3) and Serum- and Glucocorticoid-regulated Kinase (Sgk1) in Xenopus Oocytes
J. Gen. Physiol., July 30, 2002; 120(2): 191 - 201.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Bulbarelli, T. Sprocati, M. Barberi, E. Pedrazzini, and N. Borgese
Trafficking of tail-anchored proteins: transport from the endoplasmic reticulum to the plasma membrane and sorting between surface domains in polarised epithelial cells
J. Cell Sci., April 15, 2002; 115(8): 1689 - 1702.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. S. McGwire, W. A. O'Connell, K.-P. Chang, and D. M. Engman
Extracellular Release of the Glycosylphosphatidylinositol (GPI)-linked Leishmania Surface Metalloprotease, gp63, Is Independent of GPI Phospholipolysis. IMPLICATIONS FOR PARASITE VIRULENCE
J. Biol. Chem., March 8, 2002; 277(11): 8802 - 8809.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Zheng and J. E. Sadler
Mucin-like Domain of Enteropeptidase Directs Apical Targeting in Madin-Darby Canine Kidney Cells
J. Biol. Chem., February 22, 2002; 277(9): 6858 - 6863.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. Barman and D. P. Nayak
Analysis of the Transmembrane Domain of Influenza Virus Neuraminidase, a Type II Transmembrane Glycoprotein, for Apical Sorting and Raft Association
J. Virol., July 15, 2000; 74(14): 6538 - 6545.
[Abstract] [Full Text]

Home page
 J. Virol.Home page
J. Zhang, A. Pekosz, and R. A. Lamb
Influenza Virus Assembly and Lipid Raft Microdomains: a Role for the Cytoplasmic Tails of the Spike Glycoproteins
J. Virol., May 15, 2000; 74(10): 4634 - 4644.
[Abstract] [Full Text]

Home page
 Mol. Biol. CellHome page
C. Lipardi, L. Nitsch, and C. Zurzolo
Detergent-insoluble GPI-anchored Proteins Are Apically Sorted in Fischer Rat Thyroid Cells, but Interference with Cholesterol or Sphingolipids Differentially Affects Detergent Insolubility and Apical Sorting
Mol. Biol. Cell, February 1, 2000; 11(2): 531 - 542.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
N. Meerson, V Bello, J. Delaunay, T. Slimane, D Delautier, C Lenoir, G Trugnan, and M Maurice
Intracellular traffic of the ecto-nucleotide pyrophosphatase/phosphodiesterase NPP3 to the apical plasma membrane of MDCK and Caco-2 cells: apical targeting occurs in the absence of N-glycosylation
J. Cell Sci., January 12, 2000; 113(23): 4193 - 4202.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
K Prydz and K. Dalen
Synthesis and sorting of proteoglycans
J. Cell Sci., January 1, 2000; 113(2): 193 - 205.
[Abstract] [PDF]

Home page
 J. Cell Biol.Home page
J. H. Benting, A. G. Rietveld, and K. Simons
N-Glycans Mediate the Apical Sorting of a GPI-anchored, Raft-associated Protein in Madin-Darby Canine Kidney Cells
J. Cell Biol., July 26, 1999; 146(2): 313 - 320.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Martinez-Maza, I. Poyatos, B. Lopez-Corcuera, E. Nunez, C. Gimenez, F. Zafra, and C. Aragon
The Role of N-Glycosylation in Transport to the Plasma Membrane and Sorting of the Neuronal Glycine Transporter GLYT2
J. Biol. Chem., January 12, 2001; 276(3): 2168 - 2173.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. D. Hooper, J. A. Clements, J. P. Quigley, and T. M. Antalis
Type II Transmembrane Serine Proteases. INSIGHTS INTO AN EMERGING CLASS OF CELL SURFACE PROTEOLYTIC ENZYMES*
J. Biol. Chem., January 5, 2001; 276(2): 857 - 860.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.