| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 3, 1814-1820, January 15, 1999
From the Department of Cell Biology, Lerner Research Institute,
Cleveland Clinic Foundation, Cleveland, Ohio 44195
Published studies demonstrate that
lipid transfer inhibitor protein (LTIP) is an important regulator of
cholesteryl ester transfer protein (CETP) activity. Although LTIP
inhibits CETP activity among different lipoprotein classes, it
preferentially suppresses transfer events involving low density
lipoprotein (LDL), whereas transfers involving high density lipoprotein
as donor are less affected. In this study, we report the purification
of LTIP and the expression of its cDNA in cultured cells.
Purification of LTIP, in contrast to other published protocols, took
advantage of the tight association of this protein with LDL.
Ultracentrifugally isolated LDL was further purified on anti-apoE and
apoA-I affinity columns. Affinity purified LDL was delipidated by
tetramethylurea, and the tetramethylurea-soluble proteins were
separated by SDS-polyacrylamide gel electrophoresis. The protein
migrating at a molecular mass of ~33 kDa was excised from the gel and
its N-terminal amino acid sequence determined. The 14-amino acid
sequence obtained showed complete homology with the sequence deduced
for apolipoprotein F (apoF) cDNA isolated from Hep G2 cells. On
Western blots, peptide-specific antibodies raised against synthetic
fragments of apoF reacted with the same 33-kDa protein in
LTIP-containing fractions purified from LDL and from
lipoprotein-deficient plasma. In contrast to that previously reported,
apoF was shown to be associated almost exclusively with LDL, identical
to the distribution of LTIP activity. The cDNA for apoF was cloned
from a human liver cDNA library, ligated into a mammalian
expression vector, and transiently transfected into COS-7 cells.
Conditioned media containing secreted apoF demonstrated CETP inhibitor
activity, whereas cells transfected with vector alone did not. This
CETP inhibitor activity was efficiently removed from the media by
nickel-Sepharose, consistent with the 6-His tag incorporated into
recombinant apoF. By Western blot, the 6-His-tagged protein had a
molecular weight slightly larger than native apoF. The CETP inhibitor
activity of recombinant apoF possessed the same LDL specificity, oleate
sensitivity, and dependence on lipoprotein concentration as previously
noted for LTIP. We conclude that LTIP and apoF are identical.
This article has been cited by other articles:
|
|
 |
|
  R. E. Morton, H. M. Gnizak, D. J. Greene, K.-H. Cho, and V. M. Paromov Lipid transfer inhibitor protein (apolipoprotein F) concentration in normolipidemic and hyperlipidemic subjects J. Lipid Res., January 1, 2008; 49(1): 127 - 135. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. F. Tacer, D. Kuzman, M. Seliskar, D. Pompon, and D. Rozman TNF-{alpha} interferes with lipid homeostasis and activates acute and proatherogenic processes Physiol Genomics, October 19, 2007; 31(2): 216 - 227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Dumont, T. Gautier, J.-P. P. de Barros, H. Laplanche, D. Blache, P. Ducoroy, J. Fruchart, J.-C. Fruchart, P. Gambert, D. Masson, et al. Molecular Mechanism of the Blockade of Plasma Cholesteryl Ester Transfer Protein by Its Physiological Inhibitor Apolipoprotein CI J. Biol. Chem., November 11, 2005; 280(45): 38108 - 38116. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. O'Bryan, L. M. Foulds, J. F. Cannon, W. R. Winnall, J. A. Muir, K. Sebire, A. I. Smith, H.-H. Keah, M. T. W. Hearn, D. M. de Kretser, et al. Identification of a Novel Apolipoprotein, ApoN, in Ovarian Follicular Fluid Endocrinology, November 1, 2004; 145(11): 5231 - 5242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ishimori, R. Li, P. M. Kelmenson, R. Korstanje, K. A. Walsh, G. A. Churchill, K. Forsman-Semb, and B. Paigen Quantitative trait loci that determine plasma lipids and obesity in C57BL/6J and 129S1/SvImJ inbred mice J. Lipid Res., September 1, 2004; 45(9): 1624 - 1632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Bosse, Y. C. Chagnon, J-P. Despres, T. Rice, D. C. Rao, C. Bouchard, L. Perusse, and M-C. Vohl Genome-wide linkage scan reveals multiple susceptibility loci influencing lipid and lipoprotein levels in the Quebec Family Study J. Lipid Res., March 1, 2004; 45(3): 419 - 426. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Korstanje, J. J. Albers, G. Wolfbauer, R. Li, A.-Y. Tu, G. A. Churchill, and B. J. Paigen Quantitative Trait Locus Mapping of Genes That Regulate Phospholipid Transfer Activity in SM/J and NZB/BlNJ Inbred Mice Arterioscler. Thromb. Vasc. Biol., January 1, 2004; 24(1): 155 - 160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Morton and D. J. Greene CETP and lipid transfer inhibitor protein are uniquely affected by the negative charge density of the lipid and protein domains of LDL J. Lipid Res., December 1, 2003; 44(12): 2287 - 2296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. M. Paromov and R. E. Morton Lipid Transfer Inhibitor Protein Defines the Participation of High Density Lipoprotein Subfractions in Lipid Transfer Reactions Mediated by Cholesterol Ester Transfer Protein (CETP) J. Biol. Chem., October 17, 2003; 278(42): 40859 - 40866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Parini and L. L. Rudel Is There a Need for Cholesteryl Ester Transfer Protein Inhibition? Arterioscler. Thromb. Vasc. Biol., March 1, 2003; 23(3): 374 - 375. [Full Text] [PDF]
|
|
|
|
|
|
P. J. Barter, H. B. Brewer Jr, M. J. Chapman, C. H. Hennekens, D. J. Rader, and A. R. Tall Cholesteryl Ester Transfer Protein: A Novel Target for Raising HDL and Inhibiting Atherosclerosis Arterioscler. Thromb. Vasc. Biol., February 1, 2003; 23(2): 160 - 167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Gautier, D. Masson, M. C. Jong, L. Duverneuil, N. Le Guern, V. Deckert, J.-P. P. de Barros, L. Dumont, A. Bataille, Z. Zak, et al. Apolipoprotein CI Deficiency Markedly Augments Plasma Lipoprotein Changes Mediated by Human Cholesteryl Ester Transfer Protein (CETP) in CETP Transgenic/ApoCI-knocked Out Mice J. Biol. Chem., August 23, 2002; 277(35): 31354 - 31363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Skeggs and R. E. Morton LDL and HDL enriched in triglyceride promote abnormal cholesterol transport J. Lipid Res., August 1, 2002; 43(8): 1264 - 1274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Morton, V. Nunes, L. Izem, and E. Quintao Markedly Elevated Lipid Transfer Inhibitor Protein in Hypercholesterolemic Subjects Is Mitigated by Plasma Triglyceride Levels Arterioscler. Thromb. Vasc. Biol., October 1, 2001; 21(10): 1642 - 1649. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. von Eckardstein, J.-R. Nofer, and G. Assmann High Density Lipoproteins and Arteriosclerosis : Role of Cholesterol Efflux and Reverse Cholesterol Transport Arterioscler. Thromb. Vasc. Biol., January 1, 2001; 21(1): 13 - 27. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Oka, T. Kujiraoka, M. Ito, T. Egashira, S. Takahashi, M. N. Nanjee, N. E. Miller, J. Metso, V. M. Olkkonen, C. Ehnholm, et al. Distribution of phospholipid transfer protein in human plasma: presence of two forms of phospholipid transfer protein, one catalytically active and the other inactive J. Lipid Res., October 1, 2000; 41(10): 1651 - 1657. [Abstract] [Full Text]
|
|
|
|
|
|
V. Pruneta, T. Pulcini, F. Lalanne, C. Marcais, F. Berthezene, G. Ponsin, and P. Moulin VLDL-bound lipoprotein lipase facilitates the cholesteryl ester transfer protein-mediated transfer of cholesteryl esters from HDL to VLDL J. Lipid Res., December 1, 1999; 40(12): 2333 - 2339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Xu and B. Dahlback A Novel Human Apolipoprotein (apoM) J. Biol. Chem., October 29, 1999; 274(44): 31286 - 31290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Gautier, D. Masson, J.-P. P. de Barros, A. Athias, P. Gambert, D. Aunis, M.-H. Metz-Boutigue, and L. Lagrost Human Apolipoprotein C-I Accounts for the Ability of Plasma High Density Lipoproteins to Inhibit the Cholesteryl Ester Transfer Protein Activity J. Biol. Chem., November 22, 2000; 275(48): 37504 - 37509. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
