JBC Anatrace, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kawasaki, K.
Right arrow Articles by Dowhan, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kawasaki, K.
Right arrow Articles by Dowhan, W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 274, Issue 3, 1828-1834, January 15, 1999

Isolation of a Chinese Hamster Ovary (CHO) cDNA Encoding Phosphatidylglycerophosphate (PGP) Synthase, Expression of Which Corrects the Mitochondrial Abnormalities of a PGP Synthase-defective Mutant of CHO-K1 Cells

Kiyoshi KawasakiDagger §, Osamu KugeDagger , Shao-Chun Chang§, Philip N. Heacock§, Minseok Rho§, Kenji SuzukiDagger , Masahiro NishijimaDagger , and William Dowhan§

From the Dagger  Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan and the § Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston, Texas 77225

Phosphatidylglycerophosphate (PGP) synthase catalyzes the first step in the cardiolipin (CL) branch of phospholipid biosynthesis in mammalian cells. In this study, we isolated a Chinese hamster ovary (CHO) cDNA encoding a putative protein similar in sequence to the yeast PGS1 gene product, PGP synthase. The gene for the isolated CHO cDNA was named PGS1. Expression of the CHO PGS1 cDNA in CHO-K1 cells and production of a recombinant CHO PGS1 protein with a N-terminal extension in Escherichia coli resulted in 15-fold and 90-fold increases of PGP synthase specific activity, respectively, establishing that CHO PGS1 encodes PGP synthase. A PGP synthase-defective CHO mutant, PGS-S, isolated previously (Ohtsuka, T., Nishijima, M., and Akamatsu, Y. (1993) J. Biol. Chem. 268, 22908-22913) exhibits striking reductions in biosynthetic rate and cellular content of phosphatidylglycerol (PG) and CL and shows mitochondrial morphological and functional abnormalities. The CHO PGS-S mutant transfected with the CHO PGS1 cDNA exhibited 620-fold and 7-fold higher PGP synthase activity than mutant PGS-S and wild type CHO-K1 cells, respectively, and had a normal cellular content and rate of biosynthesis of PG and CL. In contrast to mutant PGS-S, the transfectant had morphologically normal mitochondria. When the transfectant and mutant PGS-S cells were cultivated in a glucose-depleted medium, in which cellular energy production mainly depends on mitochondrial function, the transformant but not mutant PGS-S was capable of growth. These results demonstrated that the morphological and functional defects displayed by the PGS-S mutant are due directly to the reduced ability to make normal levels of PG and/or CL.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
G. M. Hatch, Y. Gu, F. Y. Xu, J. Cizeau, S. Neumann, J.-S. Park, S. Loewen, and M. R.A. Mowat
StARD13(Dlc-2) RhoGap Mediates Ceramide Activation of Phosphatidylglycerolphosphate Synthase and Drug Response in Chinese Hamster Ovary Cells
Mol. Biol. Cell, March 1, 2008; 19(3): 1083 - 1092.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
F. Hullin-Matsuda, K. Kawasaki, I. Delton-Vandenbroucke, Y. Xu, M. Nishijima, M. Lagarde, M. Schlame, and T. Kobayashi
De novo biosynthesis of the late endosome lipid, bis(monoacylglycero)phosphate
J. Lipid Res., September 1, 2007; 48(9): 1997 - 2008.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Zhong, G. Li, J. Gvozdenovic-Jeremic, and M. L. Greenberg
Up-regulation of the Cell Integrity Pathway in Saccharomyces cerevisiae Suppresses Temperature Sensitivity of the pgs1{Delta} Mutant
J. Biol. Chem., June 1, 2007; 282(22): 15946 - 15953.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
X. Su and W. Dowhan
Translational Regulation of Nuclear Gene COX4 Expression by Mitochondrial Content of Phosphatidylglycerol and Cardiolipin in Saccharomyces cerevisiae
Mol. Cell. Biol., February 1, 2006; 26(3): 743 - 753.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Q. Zhong, J. Gvozdenovic-Jeremic, P. Webster, J. Zhou, and M. L. Greenberg
Loss of Function of KRE5 Suppresses Temperature Sensitivity of Mutants Lacking Mitochondrial Anionic Lipids
Mol. Biol. Cell, February 1, 2005; 16(2): 665 - 675.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Yang, J. Cao, and Y. Shi
Identification and Characterization of a Gene Encoding Human LPGAT1, an Endoplasmic Reticulum-associated Lysophosphatidylglycerol Acyltransferase
J. Biol. Chem., December 31, 2004; 279(53): 55866 - 55874.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Zhong, V. M. Gohil, L. Ma, and M. L. Greenberg
Absence of Cardiolipin Results in Temperature Sensitivity, Respiratory Defects, and Mitochondrial DNA Instability Independent of pet56
J. Biol. Chem., July 30, 2004; 279(31): 32294 - 32300.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
J. Liu, Q. Dai, J. Chen, D. Durrant, A. Freeman, T. Liu, D. Grossman, and R. M. Lee
Phospholipid Scramblase 3 Controls Mitochondrial Structure, Function, and Apoptotic Response
Mol. Cancer Res., October 1, 2003; 1(12): 892 - 902.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Zhong and M. L. Greenberg
Regulation of Phosphatidylglycerophosphate Synthase by Inositol in Saccharomyces cerevisiae Is Not at the Level of PGS1 mRNA Abundance
J. Biol. Chem., September 5, 2003; 278(36): 33978 - 33984.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Hagio, I. Sakurai, S. Sato, T. Kato, S. Tabata, and H. Wada
Phosphatidylglycerol is Essential for the Development of Thylakoid Membranes in Arabidopsis thaliana
Plant Cell Physiol., December 15, 2002; 43(12): 1456 - 1464.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
F. Valianpour, R. J.A. Wanders, P. G. Barth, H. Overmars, and A. H. van Gennip
Quantitative and Compositional Study of Cardiolipin in Platelets by Electrospray Ionization Mass Spectrometry: Application for the Identification of Barth Syndrome Patients
Clin. Chem., September 1, 2002; 48(9): 1390 - 1397.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. Kikuchi, I. Shibuya, and K. Matsumoto
Viability of an Escherichia coli pgsA Null Mutant Lacking Detectable Phosphatidylglycerol and Cardiolipin
J. Bacteriol., January 15, 2000; 182(2): 371 - 376.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
D. B. Ostrander, M. Zhang, E. Mileykovskaya, M. Rho, and W. Dowhan
Lack of Mitochondrial Anionic Phospholipids Causes an Inhibition of Translation of Protein Components of the Electron Transport Chain. A YEAST GENETIC MODEL SYSTEM FOR THE STUDY OF ANIONIC PHOSPHOLIPID FUNCTION IN MITOCHONDRIA
J. Biol. Chem., June 29, 2001; 276(27): 25262 - 25272.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.