| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 30, 20874-20878, July 23, 1999
Type II
Receptor-mediated Manner
,,,, and
From the Mesangial cells share features with contractile
smooth muscle cells and mechanically support the capillary wall. The
role of vitamin D compounds and the transforming growth factor- Division of Molecular Medicine for Adult and
Geriatric Diseases,
(TGF-) type II receptor in modulating the smooth muscle phenotype of cultured mesangial cells was examined. Cell proliferation was significantly inhibited by the vitamin D analog
22-oxa-1,25-dihydroxyvitamin D3 (22-oxacalcitriol;
OCT) rather than by 1,25-dihydroxyvitamin D3
(1,25(OH)2D3) in a dose-dependent
manner. OCT-treated early passage mesangial cells (MC-E cells) had
increased expression levels of type IV collagen and smooth muscle 
actin mRNA, but 1,25(OH)2D3-treated MC-E
cells did not. The addition of a TGF-1-neutralizing antibody to the OCT-treated MC-E cells blocked this inhibitory effect
for cell proliferation and attenuated the up-regulated mRNA levels.
However, after exposure to 1,25(OH)2D3 or OCT,
there was no significant difference in the secretion of active TGF-. We next investigated whether TGF- type II receptor (RII) was involved in this regulation. OCT treatment significantly increased the
expression of the RII mRNA in MC-E cells. These results suggest that the vitamin D analog OCT induces smooth muscle phenotypic alterations and that this phenomenon was mediated through the induction
of RII in cultured mesangial cells.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
This article has been cited by other articles:
|
|
 |
|
  S. Ohashi, H. Abe, T. Takahashi, Y. Yamamoto, M. Takeuchi, H. Arai, K. Nagata, T. Kita, H. Okamoto, H. Yamamoto, et al. Advanced Glycation End Products Increase Collagen-specific Chaperone Protein in Mouse Diabetic Nephropathy J. Biol. Chem., May 7, 2004; 279(19): 19816 - 19823. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Abe, T. Matsubara, N. Iehara, K. Nagai, T. Takahashi, H. Arai, T. Kita, and T. Doi Type IV Collagen Is Transcriptionally Regulated by Smad1 under Advanced Glycation End Product (AGE) Stimulation J. Biol. Chem., April 2, 2004; 279(14): 14201 - 14206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hirata, K. Makibayashi, K. Katsumata, K. Kusano, T. Watanabe, N. Fukushima, and T. Doi 22-Oxacalcitriol prevents progressive glomerulosclerosis without adversely affecting calcium and phosphorus metabolism in subtotally nephrectomized rats Nephrol. Dial. Transplant., December 1, 2002; 17(12): 2132 - 2137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Makibayashi, M. Tatematsu, M. Hirata, N. Fukushima, K. Kusano, S. Ohashi, H. Abe, K. Kuze, A. Fukatsu, T. Kita, et al. A Vitamin D Analog Ameliorates Glomerular Injury on Rat Glomerulonephritis Am. J. Pathol., May 1, 2001; 158(5): 1733 - 1741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Goddard, M. Bouras, M. Keramidas, J. C. Hendrick, J. J. Feige, and M. Benahmed Transforming Growth Factor-{beta} Receptor Types I and II in Cultured Porcine Leydig Cells: Expression and Hormonal Regulation Endocrinology, June 1, 2000; 141(6): 2068 - 2074. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
