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J Biol Chem, Vol. 274, Issue 30, 21049-21055, July 23, 1999
From the Maxwell Finland Laboratory for Infectious Diseases, Boston
Medical Center, Boston, Massachusetts 02118, CD14-transfected Chinese hamster ovary K1
fibroblasts (CHO/CD14) respond to lipopolysaccharide (LPS) by
metabolizing arachidonic acid and with translocation of NF-
Bacterial Lipopolysaccharide Induces Expression of the Stress
Response Genes hop and H411
, Beth
Israel Deaconess Medical Center and Harvard Medical School, Department
of Surgery, Boston, Massachusetts 02215, and the
§ Norwegian University of Science and Technology, Trondheim
7489, Norway
B to the
nucleus. Although previous experiments failed to identify the
production of tumor necrosis factor-
and interleukin (IL)-1
by
CHO/CD14 cells, LPS did induce the expression of IL-6 mRNA and the
subsequent release of the IL-6 protein. To identify additional
LPS-inducible genes, a cDNA library derived from LPS-stimulated
CHO/CD14 cells was screened by subtractive hybridization. Fourteen
genes were found to be expressed differentially, and two were analyzed
in detail: hop (Hsp70/Hsp90-organizing protein), which is
the hamster homologue of the stress-inducible yeast gene,
STI1, and clone H411, which encodes a novel
LPS-inducible growth factor. In response to LPS, the expression of Hop
mRNA was also increased in both the murine macrophage cell line,
RAW 264.7, as well as in primary hamster macrophages. This suggested
that the up-regulation of Hop expression is part of the macrophage
stress response to LPS. Clone H411 encodes a protein in the
epidermal growth factor-like repeat protein family. Overexpression of
H411 cDNA in the RAW 264.7 macrophage cell line promoted an
increased growth rate, suggesting that expression of H411 is part of
the proliferative cell response to LPS. Both Hop and H411 represent
novel gene products not previously recognized as part of the complex
biological response to endotoxin.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
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