| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 30, 21121-21127, July 23, 1999
From the Glycobiology Program, Center for Cancer and
Transplantation Biology, Children's Research Institute, Washington,
D.C. 20010 and the Departments of Pediatrics and Biochemistry/Molecular
Biology, The George Washington University School of Medicine,
Washington, D. C. 20052 and the § Lipid Cell Biology
Section, NIDDK, National Institutes of Health,
Bethesda, Maryland 20892
Ceramide is a newly discovered second messenger
that has been shown to cause cell growth arrest and apoptosis. Here, we
present evidence that exogenously added C6-ceramide
induces enlargement of late endosomes and lysosomes. 10 µM C6-ceramide caused the formation of
numerous vesicles of varying sizes (2-10 µm) in fibroblasts (3T3-L1
and 3T3-F442A), without toxic effects. Vesicle formation induced by
C6-ceramide was time- and dose-dependent,
rapid, and reversible. Numerous small vesicles appeared within 8 h
of treatment with 10 µM C6-ceramide. They
enlarged with time, with large vesicles found in the perinuclear region
and small ones observed at the cell periphery. Within 24 h of
treatment, ~30% of the cells exhibited these vesicles. Removal of
ceramide from the culture medium caused disappearance of the vesicles,
which reappeared upon readdition of ceramide. Confocal
immunofluorescence microscopic analysis using an
anti-lysosome-associated membrane protein antibody identified the
enlarged vesicles as late endosomes/lysosomes. The fluorescent C6-NBD-ceramide, a vital stain for the Golgi apparatus,
did not stain these vesicles. The effect on vesicle formation was
influenced by ceramide structure;
D-erythro-C6-ceramide was the most active ceramide analogue tested. Short chain ceramide metabolites, such as
sphingosine, sphingosine 1-phosphate,
N-hexanoyl-sphingosylphosphorylcholine, N-acetylpsychosine, and C2-ceramide
GM3, (GM3,
N-acetylneuraminosyl-
(2,3)-galactosyl-
(1,4)-glucosylceramide), were inactive in causing vesicle formation when added
exogenously. Together, these studies demonstrate that exogenous
C6-ceramide induces endocytic vesicle formation and causes
enlarged late endosomes and lysosomes in mouse fibroblasts.
This article has been cited by other articles:
|
|
 |
|
  K. Trajkovic, C. Hsu, S. Chiantia, L. Rajendran, D. Wenzel, F. Wieland, P. Schwille, B. Brugger, and M. Simons Ceramide Triggers Budding of Exosome Vesicles into Multivesicular Endosomes Science, February 29, 2008; 319(5867): 1244 - 1247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Finnegan, S. S. Rawat, E. H. Cho, D. L. Guiffre, S. Lockett, A. H. Merrill Jr., and R. Blumenthal Sphingomyelinase Restricts the Lateral Diffusion of CD4 and Inhibits Human Immunodeficiency Virus Fusion J. Virol., May 15, 2007; 81(10): 5294 - 5304. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Lopez-Montero, N. Rodriguez, S. Cribier, A. Pohl, M. Velez, and P. F. Devaux Rapid Transbilayer Movement of Ceramides in Phospholipid Vesicles and in Human Erythrocytes J. Biol. Chem., July 8, 2005; 280(27): 25811 - 25819. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Rohrbough, E. Rushton, L. Palanker, E. Woodruff, H. J. G. Matthies, U. Acharya, J. K. Acharya, and K. Broadie Ceramidase Regulates Synaptic Vesicle Exocytosis and Trafficking J. Neurosci., September 8, 2004; 24(36): 7789 - 7803. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-y. Morita, M. Kawabe, A. Sakurai, K. Okuhira, A. Vertut-Doi, M. Nakano, and T. Handa Ceramide in Lipid Particles Enhances Heparan Sulfate Proteoglycan and Low Density Lipoprotein Receptor-related Protein-mediated Uptake by Macrophages J. Biol. Chem., June 4, 2004; 279(23): 24355 - 24361. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Gurunathan, M. Marash, A. Weinberger, and J. E. Gerst t-SNARE Phosphorylation Regulates Endocytosis in Yeast Mol. Biol. Cell, May 1, 2002; 13(5): 1594 - 1607. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Shabbits and L. D. Mayer P-Glycoprotein Modulates Ceramide-mediated Sensitivity of Human Breast Cancer Cells to Tubulin-binding Anticancer Drugs Mol. Cancer Ther., January 1, 2002; 1(3): 205 - 213. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Klapisz, J. Masliah, G. Béréziat, C. Wolf, and K. S. Koumanov Sphingolipids and cholesterol modulate membrane susceptibility to cytosolic phospholipase A2 J. Lipid Res., October 1, 2000; 41(10): 1680 - 1688. [Abstract] [Full Text]
|
|
|
|
 |
|
 T Fukunaga, M Nagahama, K Hatsuzawa, K Tani, A Yamamoto, and M Tagaya Implication of sphingolipid metabolism in the stability of the Golgi apparatus J. Cell Sci., January 9, 2000; 113(18): 3299 - 3307. [Abstract] [PDF]
|
|
|
|
 |
|
 A. K.A. deHart, J. D. Schnell, D. A. Allen, and L. Hicke The conserved Pkh-Ypk kinase cascade is required for endocytosis in yeast J. Cell Biol., January 21, 2002; 156(2): 241 - 248. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
