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J Biol Chem, Vol. 274, Issue 30, 21139-21148, July 23, 1999

Overexpression of Proteins Containing Tyrosine- or Leucine-based Sorting Signals Affects Transferrin Receptor Trafficking

Tommy W. Nordeng and Oddmund Bakke

From the Division of Molecular Cell Biology, Department of Biology, University of Oslo, Oslo 0316, Norway

Targeting of many transmembrane proteins to post-Golgi compartments is dependent on cytoplasmically exposed sorting signals. The most widely used signals conform to the tyrosine- or the leucine-based motifs. Both types of signals have been implicated in protein localization to the same intracellular compartments, but previous results from both cell-free experiments and studies of transfected cell lines have indicated that the two types of signals interact with separate components of the sorting machinery. We have overexpressed several transmembrane proteins in stably transfected Madin-Darby canine kidney cells using an inducible promoter system. Overexpression of proteins containing tyrosine- or leucine-based sorting signals resulted in reduced internalization of the transferrin receptor, whereas recycling and polarized distribution was not influenced. Our results indicate that proteins with tyrosine- and leucine-based sorting signals can be transported along common saturable pathways.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
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Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.