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J Biol Chem, Vol. 274, Issue 30, 21209-21216, July 23, 1999
From the Division of Biochemistry and Nutrition, Research
Institute, International Medical Center of Japan,
Tokyo 162-8655, Japan
We made a comparative study of the structures of
the oligosaccharides on the glycoproteins from Caco-2 human colonic
adenocarcinoma cells, before and after differentiation. Enterocytic
differentiated Caco-2 cells highly express H type 1 blood group antigen
on the cell surface as well as activities of brush border membrane
hydrolases, such as dipeptidyl peptidase IV and alkaline phosphatase. A
strong correlation was observed between the amounts of H type 1 blood group antigen and the degrees of differentiation. Structural analysis with use of lectin affinity high performance liquid chromatography revealed that typical mucin-type sugar chains of the glycoproteins from
undifferentiated cells have H type 2 group, linear polylactosamines, and core 1 structure. On the other hand, differentiated cells newly
contain H type 1 and Leb groups and core 2 structure.
Mucins with H type 1 group make contact with brush border membrane
enzymes on differentiated cells. Furthermore benzyl
2-acetamide-2-deoxy-
-D-galactopyranoside inhibited both expression of H type 1 group on the cell surface and enhancement of brush border membrane enzyme activities even in the presence of a
differentiating inducer. These results suggest that the mucin-type sugar chains with H type 1 group have important functions regarding differentiation of Caco-2 cells.
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