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J Biol Chem, Vol. 274, Issue 30, 21409-21415, July 23, 1999
From the Department of Biochemistry, Faculty of Medicine, Kagoshima
University, Kagoshima 890-8520, Japan
Cadherins are transmembrane glycoproteins
involved in Ca2+-dependent cell-cell
adhesion. Previously, we showed that the conserved membrane-proximal
region of the E-cadherin cytoplasmic domain negatively regulates
adhesion activity. In this report, we provide several lines of evidence
that p120ctn is involved in this negative regulation.
p120ctn binds to the membrane-proximal region of the
nonfunctional carboxyl-terminally deleted E-cadherin protein. An
additional internal deletion in this region prevented the association
with p120ctn and activated the protein, as seen in an
aggregation assay. Furthermore, the nonfunctional E-cadherin can be
activated through coexpression of p120ctn proteins with
amino-terminal deletions, which eliminate several potential
serine/threonine phosphorylation sites but do not affect the ability to
bind to cadherins. Finally, we show that staurosporine, a kinase
inhibitor, induces an increased electrophoretic mobility of
p120ctn bound to E-cadherin polypeptides, activates the
nonfunctional E-cadherin protein, and converts the wild-type E-cadherin
and an E-cadherin-
p120ctn Binds to the Membrane-proximal Region of
the E-cadherin Cytoplasmic Domain and Is Involved in Modulation
of Adhesion Activity
-catenin chimeric protein from a cytochalasin
D-sensitive to a cytochalasin D-insensitive state. Together, these
results indicate that p120ctn is a modulator of
E-cadherin-mediated cell adhesion.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
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|
|
|
|
|
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|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
 O. Montonen, M. Aho, J. Uitto, and S. Aho Tissue Distribution and Cell Type-specific Expression of p120ctn Isoforms J. Histochem. Cytochem., December 1, 2001; 49(12): 1487 - 1496. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
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|
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|
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|
|
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|
|
|
|
|
|
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|
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