| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 30, 21416-21424, July 23, 1999
From the Diabetes Branch, NIDDK, National Institutes of Health,
Bethesda, Maryland 20892
There are four known isoforms of the human leptin
receptor (HLR) with different C-terminal cytoplasmic domains
(designated by the number of unique C-terminal amino acids). In cells
expressing HLR-5, -15, or -274, 15-25% of the leptin binding sites
were located at the plasma membrane. In contrast, in cells expressing
HLR-67, only 5% of the total binding sites were at the plasma
membrane. Immunofluorescent microscopy showed that all four isoforms
partially co-localized with calnexin and 
-COP, markers of the
endoplasmic reticulum and the Golgi, respectively. All isoforms were
also detected in an unidentified punctate compartment. All isoforms were internalized via clathrin-mediated endocytosis, but at different rates. After 20 min at 37 °C, 45% of a bound cohort of labeled ligand had been internalized by HLR-15, 30% by HLR-67, 25% by HLR-274, and 15% by HLR-5. Degradation of internalized leptin occurred
in lysosomes. Overnight exposure to leptin down-regulated all isoforms,
but to a variable extent. HLR-274 displayed the greatest
down-regulation and also appeared to reach lysosomes more quickly than
the other isoforms. The faster degradation of HLR-274 may help to
terminate leptin signaling.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. R. McGaffin, C.-K. Sun, J. J. Rager, L. C. Romano, B. Zou, M. A. Mathier, R. M. O'Doherty, C. F. McTiernan, and C. P. O'Donnell Leptin signalling reduces the severity of cardiac dysfunction and remodelling after chronic ischaemic injury Cardiovasc Res, January 1, 2008; 77(1): 54 - 63. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Couturier, C. Sarkis, K. Seron, S. Belouzard, P. Chen, A. Lenain, L. Corset, J. Dam, V. Vauthier, A. Dubart, et al. Silencing of OB-RGRP in mouse hypothalamic arcuate nucleus increases leptin receptor signaling and prevents diet-induced obesity PNAS, December 4, 2007; 104(49): 19476 - 19481. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Yang and L. A. Barouch Leptin Signaling and Obesity: Cardiovascular Consequences Circ. Res., September 14, 2007; 101(6): 545 - 559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Magarinos, V. Sanchez-Margalet, M. Kotler, J. C. Calvo, and C. L. Varone Leptin Promotes Cell Proliferation and Survival of Trophoblastic Cells Biol Reprod, February 1, 2007; 76(2): 203 - 210. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zerani, C. Boiti, D. Zampini, G. Brecchia, C. Dall'Aglio, P. Ceccarelli, and A. Gobbetti Ob receptor in rabbit ovary and leptin in vitro regulation of corpora lutea J. Endocrinol., November 1, 2004; 183(2): 279 - 288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Edwards, R. P. Bohm Jr, J. Purcell, M. S. Ratterree, K. F. Swan, V. D. Castracane, and M. C. Henson Two Isoforms of the Leptin Receptor Are Enhanced in Pregnancy-Specific Tissues and Soluble Leptin Receptor Is Enhanced in Maternal Serum with Advancing Gestation in the Baboon Biol Reprod, November 1, 2004; 71(5): 1746 - 1752. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Garofalo, D. Sisci, and E. Surmacz Leptin Interferes with the Effects of the Antiestrogen ICI 182,780 in MCF-7 Breast Cancer Cells Clin. Cancer Res., October 1, 2004; 10(19): 6466 - 6475. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Belouzard, D. Delcroix, and Y. Rouille Low Levels of Expression of Leptin Receptor at the Cell Surface Result from Constitutive Endocytosis and Intracellular Retention in the Biosynthetic Pathway J. Biol. Chem., July 2, 2004; 279(27): 28499 - 28508. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Yang, H. Ge, A. Boucher, X. Yu, and C. Li Modulation of Direct Leptin Signaling by Soluble Leptin Receptor Mol. Endocrinol., June 1, 2004; 18(6): 1354 - 1362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Camina, M. C. Carreira, S. El Messari, C. Llorens-Cortes, R. G. Smith, and F. F. Casanueva Desensitization and Endocytosis Mechanisms of Ghrelin-Activated Growth Hormone Secretagogue Receptor 1a Endocrinology, February 1, 2004; 145(2): 930 - 940. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Bjorbaek and B. B. Kahn Leptin Signaling in the Central Nervous System and the Periphery Recent Prog. Horm. Res., January 1, 2004; 59(1): 305 - 331. [Abstract] [Full Text]
|
|
|
|
|
|
C. Couturier and R. Jockers Activation of the Leptin Receptor by a Ligand-induced Conformational Change of Constitutive Receptor Dimers J. Biol. Chem., July 11, 2003; 278(29): 26604 - 26611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Coatmellec-Taglioni, J.-P. Dausse, Y. Giudicelli, and C. Ribiere Sexual Dimorphism in Cafeteria Diet-Induced Hypertension Is Associated with Gender-Related Difference in Renal Leptin Receptor Down-Regulation J. Pharmacol. Exp. Ther., April 1, 2003; 305(1): 362 - 367. [Abstract] [Full Text]
|
|
|
|
|
|
A. M. Rojkova, G. E. Woodard, T.-C. Huang, C. A. Combs, J.-H. Zhang, and W. F. Simonds Ggamma Subunit-selective G Protein beta 5 Mutant Defines Regulators of G Protein Signaling Protein Binding Requirement for Nuclear Localization J. Biol. Chem., March 28, 2003; 278(14): 12507 - 12512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-C. Lu, P. Scott, G. J. Strous, and L. A. Schuler Multiple Internalization Motifs Differentially Used by Prolactin Receptor Isoforms Mediate Similar Endocytic Pathways Mol. Endocrinol., November 1, 2002; 16(11): 2515 - 2527. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Barrenetxe, A C Villaro, L Guembe, I Pascual, M Munoz-Navas, A Barber, and M P Lostao Distribution of the long leptin receptor isoform in brush border, basolateral membrane, and cytoplasm of enterocytes Gut, June 1, 2002; 50(6): 797 - 802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bahrenberg, I. Behrmann, A. Barthel, P. Hekerman, P. C. Heinrich, H.-G. Joost, and W. Becker Identification of the Critical Sequence Elements in the Cytoplasmic Domain of Leptin Receptor Isoforms Required for Janus Kinase/Signal Transducer and Activator of Transcription Activation by Receptor Heterodimers Mol. Endocrinol., April 1, 2002; 16(4): 859 - 872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Maamra, M. Bidlingmaier, M.-C. Postel-Vinay, Z. Wu, C. J. Strasburger, and R. J. M. Ross Generation of Human Soluble Leptin Receptor by Proteolytic Cleavage of Membrane-Anchored Receptors Endocrinology, October 1, 2001; 142(10): 4389 - 4393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tena-Sempere, L. Pinilla, F.-P. Zhang, L. C. González, I. Huhtaniemi, F. F. Casanueva, C. Dieguez, and E. Aguilar Developmental and Hormonal Regulation of Leptin Receptor (Ob-R) Messenger Ribonucleic Acid Expression in Rat Testis Biol Reprod, February 1, 2001; 64(2): 634 - 643. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. Fantuzzi and R. Faggioni Leptin in the regulation of immunity, inflammation, and hematopoiesis J. Leukoc. Biol., October 1, 2000; 68(4): 437 - 446. [Abstract] [Full Text]
|
|
|
|
|
|
B. V. Zlokovic, S. Jovanovic, W. Miao, S. Samara, S. Verma, and C. L. Farrell Differential Regulation of Leptin Transport by the Choroid Plexus and Blood-Brain Barrier and High Affinity Transport Systems for Entry into Hypothalamus and Across the Blood-Cerebrospinal Fluid Barrier Endocrinology, April 1, 2000; 141(4): 1434 - 1441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Zhang, V. A. Barr, Y. Mo, A. M. Rojkova, S. Liu, and W. F. Simonds Nuclear Localization of G Protein beta 5 and Regulator of G Protein Signaling 7 in Neurons and Brain J. Biol. Chem., March 23, 2001; 276(13): 10284 - 10289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Parhami, Y. Tintut, A. Ballard, A. M. Fogelman, and L. L. Demer Leptin Enhances the Calcification of Vascular Cells : Artery Wall as a Target of Leptin Circ. Res., May 11, 2001; 88(9): 954 - 960. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
