Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lin, H. H.
Right arrow Articles by Ann, D. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lin, H. H.
Right arrow Articles by Ann, D. K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 274, Issue 31, 21544-21554, July 30, 1999

The Gene Expression of the Amiloride-sensitive Epithelial Sodium Channel alpha -Subunit Is Regulated by Antagonistic Effects between Glucocorticoid Hormone and Ras Pathways in Salivary Epithelial Cells

H. Helen LinDagger , Mark D. ZentnerDagger , Huei-Li Lily HoDagger , Kwang-Jin KimDagger parallel **Dagger Dagger , and David K. AnnDagger §§

From the Departments of Dagger  Molecular Pharmacology and Toxicology,  Medicine, parallel  Physiology and Biophysics, and ** Biomedical Engineering, Dagger Dagger  Will Rogers Institute Pulmonary Research Center, and §§ Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, California 90089

The functional expression of the amiloride-sensitive epithelial sodium channel (ENaC) in select epithelia is critical for maintaining electrolyte and fluid homeostasis. Although ENaC activity is strictly dependent upon its alpha -subunit expression, little is known about the molecular mechanisms by which cells modulate alpha -ENaC gene expression. Previously, we have shown that salivary alpha -ENaC expression is transcriptionally repressed by the activation of Raf/extracellular signal-regulated protein kinase pathway. Here, this work further investigates the molecular mechanism(s) by which alpha -ENaC expression is regulated in salivary epithelial Pa-4 cells. A region located between -1.5 and -1.0 kilobase pairs of the alpha -ENaC 5'-flanking region is demonstrated to be indispensable for the maximal and Ras-repressible reporter expression. Deletional analyses using heterologous promoter constructs reveal that a DNA sequence between -1355 and -1269 base pairs functions as an enhancer conferring the high level of expression on reporter constructs, and this induction effect is inhibited by Ras pathway activation. Mutational analyses indicate that full induction and Ras-mediated repression require a glucocorticoid response element (GRE) located between -1323 and -1309 base pairs. The identified alpha -ENaC GRE encompassing sequence (-1334/-1306) is sufficient to confer glucocorticoid receptor/dexamethasone-dependent and Ras-repressible expression on both heterologous and homologous promoters. This report demon- strates for the first time that the cross-talk between glucocorticoid receptor and Ras/extracellular signal-regulated protein kinase signaling pathways results in an antagonistic effect at the transcriptional level to modulate alpha -ENaC expression through the identified GRE. In summary, this study presents a mechanism by which alpha -ENaC expression is regulated in salivary epithelial cells.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Physiol.Home page
M. Gallacher, S. G. Brown, B. G. Hale, R. Fearns, R. E. Olver, R. E. Randall, and S. M. Wilson
Cation currents in human airway epithelial cells induced by infection with influenza A virus
J. Physiol., July 1, 2009; 587(13): 3159 - 3173.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. Xu and S. Chu
ENaC {alpha}-subunit variants are expressed in lung epithelial cells and are suppressed by oxidative stress
Am J Physiol Lung Cell Mol Physiol, December 1, 2007; 293(6): L1454 - L1462.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
H. Xu, T. J. Ferro, and S. Chu
Cigarette smoke condensate inhibits ENaC {alpha}-subunit expression in lung epithelial cells
Eur. Respir. J., October 1, 2007; 30(4): 633 - 642.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. R. Quesnell, X. Han, and B. D. Schultz
Glucocorticoids stimulate ENaC upregulation in bovine mammary epithelium
Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1739 - C1745.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Dagenais, R. Frechette, M.-E. Clermont, C. Masse, A. Prive, E. Brochiero, and Y. Berthiaume
Dexamethasone inhibits the action of TNF on ENaC expression and activity
Am J Physiol Lung Cell Mol Physiol, December 1, 2006; 291(6): L1220 - L1231.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
T. Ohkuri, K. Yasumatsu, N. Shigemura, R. Yoshida, and Y. Ninomiya
Amiloride Inhibition on NaCl Responses of the Chorda Tympani Nerve in Two 129 Substrains of Mice, 129P3/J and 129X1/SvJ
Chem Senses, July 1, 2006; 31(6): 565 - 572.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
I. E. Veizis and C. U. Cotton
Abnormal EGF-dependent regulation of sodium absorption in ARPKD collecting duct cells
Am J Physiol Renal Physiol, March 1, 2005; 288(3): F474 - F482.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Michlig, A. Mercier, A. Doucet, L. Schild, J.-D. Horisberger, B. C. Rossier, and D. Firsov
ERK1/2 Controls Na,K-ATPase Activity and Transepithelial Sodium Transport in the Principal Cell of the Cortical Collecting Duct of the Mouse Kidney
J. Biol. Chem., December 3, 2004; 279(49): 51002 - 51012.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. E. Booth and J. D. Stockand
Targeted degradation of ENaC in response to PKC activation of the ERK1/2 cascade
Am J Physiol Renal Physiol, May 1, 2003; 284(5): F938 - F947.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
O. A. Itani, K. L. Cornish, K. Z. Liu, and C. P. Thomas
Cycloheximide increases glucocorticoid-stimulated alpha -ENaC mRNA in collecting duct cells by p38 MAPK-dependent pathway
Am J Physiol Renal Physiol, April 1, 2003; 284(4): F778 - F787.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J.-P. Shen and C. U. Cotton
Epidermal growth factor inhibits amiloride-sensitive sodium absorption in renal collecting duct cells
Am J Physiol Renal Physiol, January 1, 2003; 284(1): F57 - F64.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
O. A. Itani, K. Z. Liu, K. L. Cornish, J. R. Campbell, and C. P. Thomas
Glucocorticoids stimulate human sgk1 gene expression by activation of a GRE in its 5'-flanking region
Am J Physiol Endocrinol Metab, November 1, 2002; 283(5): E971 - E979.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
E. Hendron and J. D. Stockand
Activation of Mitogen-activated Protein Kinase (Mitogen-activated Protein Kinase/Extracellular Signal-regulated Kinase) Cascade by Aldosterone
Mol. Biol. Cell, September 1, 2002; 13(9): 3042 - 3054.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Shi, C. Asher, A. Chigaev, Y. Yung, E. Reuveny, R. Seger, and H. Garty
Interactions of beta and gamma ENaC with Nedd4 Can Be Facilitated by an ERK-mediated Phosphorylation
J. Biol. Chem., April 12, 2002; 277(16): 13539 - 13547.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. D. Stockand
New ideas about aldosterone signaling in epithelia
Am J Physiol Renal Physiol, April 1, 2002; 282(4): F559 - F576.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Dagenais, C. Denis, M.-F. Vives, S. Girouard, C. Masse, T. Nguyen, T. Yamagata, C. Grygorczyk, R. Kothary, and Y. Berthiaume
Modulation of {alpha}-ENaC and {alpha}1-Na+-K+-ATPase by cAMP and dexamethasone in alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol, July 1, 2001; 281(1): L217 - L230.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
V. E. Mick, O. A. Itani, R. W. Loftus, R. F. Husted, T. J. Schmidt, and C. P. Thomas
The {{alpha}}-Subunit of the Epithelial Sodium Channel Is an Aldosterone-Induced Transcript in Mammalian Collecting Ducts, and This Transcriptional Response Is Mediated via Distinct cis-Elements in the 5'-Flanking Region of the Gene
Mol. Endocrinol., April 1, 2001; 15(4): 575 - 588.
[Abstract] [Full Text]

Home page
 ADRHome page
X. Wen, H.H. Lin, and D.K. Ann
Salivary Cellular Signaling and Gene Regulation
Advances in Dental Research, December 1, 2000; 14(1): 76 - 80.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
H.-C. Wang, M. D. Zentner, H.-T. Deng, K.-J. Kim, R. Wu, P.-C. Yang, and D. K. Ann
Oxidative Stress Disrupts Glucocorticoid Hormone-dependent Transcription of the Amiloride-sensitive Epithelial Sodium Channel alpha -Subunit in Lung Epithelial Cells through ERK-dependent and Thioredoxin-sensitive Pathways
J. Biol. Chem., March 17, 2000; 275(12): 8600 - 8609.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. D. Zentner, H. H. Lin, H.-T. Deng, K.-J. Kim, H.-M. Shih, and D. K. Ann
Requirement for High Mobility Group Protein HMGI-C Interaction with STAT3 Inhibitor PIAS3 in Repression of alpha -Subunit of Epithelial Na+ Channel (alpha -ENaC) Transcription by Ras Activation in Salivary Epithelial Cells
J. Biol. Chem., August 3, 2001; 276(32): 29805 - 29814.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
O. A. Itani, S. D. Auerbach, R. F. Husted, K. A. Volk, S. Ageloff, M. A. Knepper, J. B. Stokes, and C. P. Thomas
Alveolar Epithelial Ion and Fluid Transport: Glucocorticoid-stimulated lung epithelial Na+ transport is associated with regulated ENaC and sgk1 expression
Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L631 - L641.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. P. Thomas, R. W. Loftus, K. Z. Liu, and O. A. Itani
Genomic organization of the 5' end of human beta -ENaC and preliminary characterization of its promoter
Am J Physiol Renal Physiol, May 1, 2002; 282(5): F898 - F909.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement