|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 31, 21603-21608, July 30, 1999
From the Laboratory of Infectious Diseases, Department of Clinical
Biochemistry, Faculty of Health Sciences, Ben-Gurion University of
the Negev and Soroka Medical Center, Beer-Sheva 84105, Israel
The NADPH oxidase-producing superoxide is the
major mechanism by which phagocytes kill invading pathogens. We
previously established a model of cytosolic phospholipase
A2 (cPLA2)-deficient differentiated PLB-985 cells (PLB-D cells) and demonstrated that
cPLA2-generated arachidonic acid (AA) is essential for
NADPH oxidase activation (Dana, R., Leto, T., Malech, H., and Levy, R. (1998) J. Biol. Chem. 273, 441-445). In the present
study, we used this model to determine the physiological role of
cPLA2 in the regulation of both the H+ channel
and the Na+/H+ antiporter and to study whether
NADPH oxidase activation is regulated by either of these transporters.
PLB-D cells and two controls: parent PLB-985 cells and PLB-985 cells
transfected with the vector only (PLB cells) were differentiated using
1.25% Me2SO or 5 × 10
Essential Requirement of Cytosolic Phospholipase A2
for Activation of the H+ Channel in Phagocyte-like
Cells
8 M
1,25-dihydroxyvitamin D3. Activation of differentiated PLB cells resulted in a Zn2+-sensitive alkalization, indicating
H+ channel activity. In contrast, differentiated PLB-D
cells failed to activate the H+ channel, but the addition
of exogenous AA fully restored this activity, indicating the role of
cPLA2 in H+ channel activation. The presence of
the H+ channel inhibitor Zn2+ caused
significant inhibition of NADPH oxidase activity, suggesting a role of
the H+ channel in regulating oxidase activity.
Na+/H+ antiporter activity was stimulated in
differentiated PLB-D cells, indicating that cPLA2 does not
participate in the regulation of this antiporter. These results
establish an essential and specific physiological requirement of
cPLA2-generated AA for activation of the H+
channel and suggest the participation of this channel in the regulation
of NADPH oxidase activity.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  E. K. Hoffmann, I. H. Lambert, and S. F. Pedersen Physiology of Cell Volume Regulation in Vertebrates Physiol Rev, January 1, 2009; 89(1): 193 - 277. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Shmelzer, M. Karter, M. Eisenstein, T. L. Leto, N. Hadad, D. Ben-Menahem, D. Gitler, S. Banani, B. Wolach, M. Rotem, et al. Cytosolic Phospholipase A2{alpha} Is Targeted to the p47phox-PX Domain of the Assembled NADPH Oxidase via a Novel Binding Site in Its C2 Domain J. Biol. Chem., November 14, 2008; 283(46): 31898 - 31908. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Brechard and E. J. Tschirhart Regulation of superoxide production in neutrophils: role of calcium influx J. Leukoc. Biol., November 1, 2008; 84(5): 1223 - 1237. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Morgan, V. V. Cherny, A. Finnegan, J. Bollinger, M. H. Gelb, and T. E. DeCoursey Sustained activation of proton channels and NADPH oxidase in human eosinophils and murine granulocytes requires PKC but not cPLA2{alpha} activity J. Physiol., March 1, 2007; 579(2): 327 - 344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Pessach, Z. Shmelzer, T. L. Leto, M. C. Dinauer, and R. Levy The C-terminal flavin domain of gp91phox bound to plasma membranes of granulocyte-like X-CGD PLB-985 cells is sufficient to anchor cytosolic oxidase components and support NADPH oxidase-associated diaphorase activity independent of cytosolic phospholipase A2 regulation J. Leukoc. Biol., September 1, 2006; 80(3): 630 - 639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Hazan-Eitan, Y. Weinstein, N. Hadad, A. Konforty, and R. Levy Induction of Fc{gamma}RIIA expression in myeloid PLB cells during differentiation depends on cytosolic phospholipase A2 activity and is regulated via activation of CREB by PGE2 Blood, September 1, 2006; 108(5): 1758 - 1766. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K. Femling, V. V. Cherny, D. Morgan, B. Rada, A. P. Davis, G. Czirjak, P. Enyedi, S. K. England, J. G. Moreland, E. Ligeti, et al. The Antibacterial Activity of Human Neutrophils and Eosinophils Requires Proton Channels but Not BK Channels J. Gen. Physiol., May 30, 2006; 127(6): 659 - 672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. R. Sheppard, M. R. Kelher, E. E. Moore, N. J. D. McLaughlin, A. Banerjee, and C. C. Silliman Structural organization of the neutrophil NADPH oxidase: phosphorylation and translocation during priming and activation J. Leukoc. Biol., November 1, 2005; 78(5): 1025 - 1042. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. C. Serezani, D. M. Aronoff, S. Jancar, and M. Peters-Golden Leukotriene B4 mediates p47phox phosphorylation and membrane translocation in polyunsaturated fatty acid-stimulated neutrophils J. Leukoc. Biol., October 1, 2005; 78(4): 976 - 984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. F. Liberty, L. Raichel, Z. Hazan-Eitan, I. Pessach, N. Hadad, F. Schlaeffer, and R. Levy Cytosolic phospholipase A2 is responsible for prostaglandin E2 and leukotriene B4 formation in phagocyte-like PLB-985 cells: studies of differentiated cPLA2-deficient PLB-985 cells J. Leukoc. Biol., July 1, 2004; 76(1): 176 - 184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. E. DeCoursey During the Respiratory Burst, Do Phagocytes Need Proton Channels or Potassium Channels, or Both? Sci. Signal., May 18, 2004; 2004(233): pe21 - pe21. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Shmelzer, N. Haddad, E. Admon, I. Pessach, T. L. Leto, Z. Eitan-Hazan, M. Hershfinkel, and R. Levy Unique targeting of cytosolic phospholipase A2 to plasma membranes mediated by the NADPH oxidase in phagocytes J. Cell Biol., August 18, 2003; 162(4): 683 - 692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Decoursey Voltage-Gated Proton Channels and Other Proton Transfer Pathways Physiol Rev, April 1, 2003; 83(2): 475 - 579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Henderson and R. W. Meech Proton Conduction through gp91phox J. Gen. Physiol., November 25, 2002; 120(6): 759 - 765. [Full Text] [PDF]
|
|
|
|
|
|
N. Touret and S. Grinstein Voltage-gated Proton "Channels": a Spectator's Viewpoint J. Gen. Physiol., November 25, 2002; 120(6): 767 - 771. [Full Text] [PDF]
|
|
|
|
|
|
T. Schilling, A. Gratopp, T. E DeCoursey, and C. Eder Voltage-activated proton currents in human lymphocytes J. Physiol., November 15, 2002; 545(1): 93 - 105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhao, E. A. Bey, F. B. Wientjes, and M. K. Cathcart Cytosolic Phospholipase A2 (cPLA2) Regulation of Human Monocyte NADPH Oxidase Activity. cPLA2 AFFECTS TRANSLOCATION BUT NOT PHOSPHORYLATION OF p67phox AND p47phox J. Biol. Chem., July 5, 2002; 277(28): 25385 - 25392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Morgan, V. V. Cherny, M. O. Price, M. C. Dinauer, and T. E. DeCoursey Absence of Proton Channels in COS-7 Cells Expressing Functional NADPH Oxidase Components J. Gen. Physiol., May 28, 2002; 119(6): 571 - 580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V V Cherny, L M Henderson, W Xu, L L Thomas, and T E DeCoursey Activation of NADPH oxidase-related proton and electron currents in human eosinophils by arachidonic acid J. Physiol., September 15, 2001; 535(3): 783 - 794. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Mollapour, D. C. Linch, and P. J. Roberts Activation and priming of neutrophil nicotinamide adenine dinucleotide phosphate oxidase and phospholipase A2 are dissociated by inhibitors of the kinases p42ERK2 and p38SAPK and by methyl arachidonyl fluorophosphonate, the dual inhibitor of cytosolic and calcium-independent phospholipase A2 Blood, April 15, 2001; 97(8): 2469 - 2477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Maturana, S. Arnaudeau, S. Ryser, B. Banfi, J. P. Hossle, W. Schlegel, K.-H. Krause, and N. Demaurex Heme Histidine Ligands within gp91phox Modulate Proton Conduction by the Phagocyte NADPH Oxidase J. Biol. Chem., August 3, 2001; 276(32): 30277 - 30284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Pessach, T. L. Leto, H. L. Malech, and R. Levy Essential Requirement of Cytosolic Phospholipase A2 for Stimulation of NADPH Oxidase-associated Diaphorase Activity in Granulocyte-like Cells J. Biol. Chem., August 31, 2001; 276(36): 33495 - 33503. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. E. DeCoursey, V. V. Cherny, W. Zhou, and L. L. Thomas Simultaneous activation of NADPH oxidase-related proton and electron currents in human neutrophils PNAS, June 6, 2000; 97(12): 6885 - 6889. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |