HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Geisbrecht, B. V. Articles by Gould, S. J. Search for Related Content PubMed PubMed Citation Articles by Geisbrecht, B. V. Articles by Gould, S. J.

J Biol Chem, Vol. 274, Issue 31, 21797-21803, July 30, 1999

Characterization of PECI, a Novel Monofunctional ³,²-Enoyl-CoA Isomerase of Mammalian Peroxisomes

Brian V. Geisbrecht, Dongyan Zhang^§, Horst Schulz^§, and Stephen J. Gould

From the  Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 and the ^§ Department of Chemistry, The City College of The City University of New York, New York, 10031

We report here the identification and characterization of human and mouse PECI, a novel gene that encodes a monofunctional peroxisomal ³,²-enoyl-CoA isomerase. Human and mouse PECI were identified on the basis of their sequence similarity to Eci1p, a recently characterized peroxisomal ³,²-enoyl-CoA isomerase from the yeast Saccharomyces cerevisiae. Cloning and sequencing of the human PECI cDNA revealed the presence of a 1077-base pair open reading frame predicted to encode a 359-amino acid protein with a mass of 39.6 kDa. The corresponding mouse cDNA contains a 1074-base pair open reading frame that encodes a 358-amino acid-long protein with a deduced mass of 39.4 kDa. Northern blot analysis demonstrated human PECI mRNA is expressed in all tissues. A bacterially expressed form of human PECI catalyzed the isomerization of 3-cis-octenoyl-CoA to 2-trans-octenoyl-CoA with a specific activity of 27 units/mg of protein. The human and mouse PECI proteins contain type-1 peroxisomal targeting signals, and human PECI was localized to peroxisomes by both subcellular fractionation and immunofluorescence microscopy techniques. The potential roles for this monofunctional ³,²-enoyl-CoA isomerase in peroxisomal metabolism are discussed.

---

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.

This article has been cited by other articles:

				S. Reumann, L. Babujee, C. Ma, S. Wienkoop, T. Siemsen, G. E. Antonicelli, N. Rasche, F. Luder, W. Weckwerth, and O. Jahn Proteome Analysis of Arabidopsis Leaf Peroxisomes Reveals Novel Targeting Peptides, Metabolic Pathways, and Defense Mechanisms PLANT CELL, October 1, 2007; 19(10): 3170 - 3193. [Abstract] [Full Text] [PDF]

				Z.-H. Tang, P. Xiao, S.-F. Lei, F.-Y. Deng, L.-J. Zhao, H.-Y. Deng, L.-J. Tan, H. Shen, D.-H. Xiong, R. R. Recker, et al. A Bivariate Whole-Genome Linkage Scan Suggests Several Shared Genomic Regions for Obesity and Osteoporosis J. Clin. Endocrinol. Metab., July 1, 2007; 92(7): 2751 - 2757. [Abstract] [Full Text] [PDF]

				B. Zeng, X. Cai, and G. Zhu Functional characterization of a fatty acyl-CoA-binding protein (ACBP) from the apicomplexan Cryptosporidium parvum. Microbiology, August 1, 2006; 152(Pt 8): 2355 - 2363. [Abstract] [Full Text] [PDF]

				A. T. De Souza, P. D. Cornwell, X. Dai, M. J. Caguyong, and R. G. Ulrich Agonists of the Peroxisome Proliferator-Activated Receptor Alpha Induce a Fiber-Type-Selective Transcriptional Response in Rat Skeletal Muscle Toxicol. Sci., August 1, 2006; 92(2): 578 - 586. [Abstract] [Full Text] [PDF]

				K. Hayashi and T. E. Spencer Estrogen Disruption of Neonatal Ovine Uterine Development: Effects on Gene Expression Assessed by Suppression Subtraction Hybridization Biol Reprod, October 1, 2005; 73(4): 752 - 760. [Abstract] [Full Text] [PDF]

				B. V. Geisbrecht, B. Y. Hamaoka, B. Perman, A. Zemla, and D. J. Leahy The Crystal Structures of EAP Domains from Staphylococcus aureus Reveal an Unexpected Homology to Bacterial Superantigens J. Biol. Chem., April 29, 2005; 280(17): 17243 - 17250. [Abstract] [Full Text] [PDF]

				X. Feng, T. Chuhjo, C. Sugimori, T. Kotani, X. Lu, A. Takami, H. Takamatsu, H. Yamazaki, and S. Nakao Diazepam-binding inhibitor-related protein 1: a candidate autoantigen in acquired aplastic anemia patients harboring a minor population of paroxysmal nocturnal hemoglobinuria-type cells Blood, October 15, 2004; 104(8): 2425 - 2431. [Abstract] [Full Text] [PDF]

				Y. Ren, J. Aguirre, A. G. Ntamack, C. Chu, and H. Schulz An Alternative Pathway of Oleate {beta}-Oxidation in Escherichia coli Involving the Hydrolysis of a Dead End Intermediate by a Thioesterase J. Biol. Chem., March 19, 2004; 279(12): 11042 - 11050. [Abstract] [Full Text] [PDF]

				B. V. Geisbrecht, K. A. Dowd, R. W. Barfield, P. A. Longo, and D. J. Leahy Netrin Binds Discrete Subdomains of DCC and UNC5 and Mediates Interactions between DCC and Heparin J. Biol. Chem., August 29, 2003; 278(35): 32561 - 32568. [Abstract] [Full Text] [PDF]

				C. C. Harper, J. M. Berg, and S. J. Gould PEX5 Binds the PTS1 Independently of Hsp70 and the Peroxin PEX12 J. Biol. Chem., February 28, 2003; 278(10): 7897 - 7901. [Abstract] [Full Text] [PDF]

				Y. Ren and H. Schulz Metabolic Functions of the Two Pathways of Oleate beta -Oxidation Double Bond Metabolism During the beta -Oxidation of Oleic Acid in Rat Heart Mitochondria J. Biol. Chem., January 3, 2003; 278(1): 111 - 116. [Abstract] [Full Text] [PDF]

				D. Zhang, W. Yu, B. V. Geisbrecht, S. J. Gould, H. Sprecher, and H. Schulz Functional Characterization of Delta 3,Delta 2-Enoyl-CoA Isomerases from Rat Liver J. Biol. Chem., March 8, 2002; 277(11): 9127 - 9132. [Abstract] [Full Text] [PDF]

				G. Dodt, D. Warren, E. Becker, P. Rehling, and S. J. Gould Domain Mapping of Human PEX5 Reveals Functional and Structural Similarities to Saccharomyces cerevisiae Pex18p and Pex21p J. Biol. Chem., November 2, 2001; 276(45): 41769 - 41781. [Abstract] [Full Text] [PDF]

				B. Gaigg, T. B. F. Neergaard, R. Schneiter, J. K. Hansen, N. J. Færgeman, N. A. Jensen, J. R. Andersen, J. Friis, R. Sandhoff, H. D. Schrøder, et al. Depletion of Acyl-Coenzyme A-Binding Protein Affects Sphingolipid Synthesis and Causes Vesicle Accumulation and Membrane Defects in Saccharomyces cerevisiae Mol. Biol. Cell, April 1, 2001; 12(4): 1147 - 1160. [Abstract] [Full Text]

				J. M. Jones, J. C. Morrell, and S. J. Gould Identification and Characterization of HAOX1, HAOX2, and HAOX3, Three Human Peroxisomal 2-Hydroxy Acid Oxidases J. Biol. Chem., April 21, 2000; 275(17): 12590 - 12597. [Abstract] [Full Text] [PDF]

				K. A. Sacksteder, J. M. Jones, S. T. South, X. Li, Y. Liu, and S. J. Gould PEX19 Binds Multiple Peroxisomal Membrane Proteins, Is Predominantly Cytoplasmic, and Is Required for Peroxisome Membrane Synthesis J. Cell Biol., March 6, 2000; 148(5): 931 - 944. [Abstract] [Full Text] [PDF]

				B. V. Geisbrecht and S. J. Gould The Human PICD Gene Encodes a Cytoplasmic and Peroxisomal NADP+-dependent Isocitrate Dehydrogenase J. Biol. Chem., October 22, 1999; 274(43): 30527 - 30533. [Abstract] [Full Text] [PDF]

				O. Bousquet, L. Ma, S. Yamada, C. Gu, T. Idei, K. Takahashi, D. Wirtz, and P. A. Coulombe The nonhelical tail domain of keratin 14 promotes filament bundling and enhances the mechanical properties of keratin intermediate filaments in vitro J. Cell Biol., November 26, 2001; 155(5): 747 - 754. [Abstract] [Full Text] [PDF]