HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kumar, M. B. Articles by Perdew, G. H. Search for Related Content PubMed PubMed Citation Articles by Kumar, M. B. Articles by Perdew, G. H. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 32, 22155-22164, August 6, 1999

Differential Recruitment of Coactivator RIP140 by Ah and Estrogen Receptors
ABSENCE OF A ROLE FOR LXXLL MOTIFS

Mohan B. Kumar^§, Rex W. Tarpey, and Gary H. Perdew^¶

From the ^§ Graduate Program in Biochemistry and Molecular Biology, the  Center for Molecular Toxicology, and the ^¶ Department of Veterinary Science, Pennsylvania State University, University Park, Pennsylvania 16802

The Ah receptor (AhR), a soluble cytosolic protein, mediates most of the toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and related environmental contaminants. The mechanism of ligand-mediated AhR activation has been, in part, elucidated. The sequence of events following the binding of the AhR/AhR nuclear translocator protein (ARNT) heterodimer to dioxin response elements has yet to be completely understood. The role of coactivator, RIP140, in the modulation of transcriptional activity of AhR/ARNT heterodimer was examined. RIP140 enhanced TCDD-mediated, dioxin response element-driven reporter gene activity in three cell lines. Co-immunoprecipitation and co-localization assays revealed that RIP140 interacted with AhR, but not with ARNT, both in vitro and in cells. Mapping of the interaction sites revealed that RIP140 was recruited by the AhR transactivation domain via the Q-rich subdomain. The RIP140 domain that interacts with the AhR was mapped to a location between amino acid residues 154 and 350, which is distinct from those involved in estrogen receptor binding. The signature motif, LXXLL, which is responsible for binding of several coactivators to nuclear receptors, is not required for RIP140 binding to AhR. These results demonstrate that the AhR recruits coactivators that are capable of enhancing transcription and, thus, the AhR may compete with steroid receptors for a common coactivator pool. In addition, the data suggest that there are distinct motif(s) for the recruitment of RIP140 to AhR and possibly other non-steroid receptors/transcription factors.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				I. Zschiedrich, U. Hardeland, A. Krones-Herzig, M. Berriel Diaz, A. Vegiopoulos, J. Muggenburg, D. Sombroek, T. G. Hofmann, R. Zawatzky, X. Yu, et al. Coactivator function of RIP140 for NF{kappa}B/RelA-dependent cytokine gene expression Blood, July 15, 2008; 112(2): 264 - 276. [Abstract] [Full Text] [PDF]

				N. A. Lewis, T. D. Williams, and J. K. Chipman Functional Analysis of a Metal Response Element in the Regulatory Region of Flounder Cytochrome P450 1A and Implications for Environmental Monitoring of Pollutants Toxicol. Sci., August 1, 2006; 92(2): 387 - 393. [Abstract] [Full Text] [PDF]

				S. Carascossa, J. Gobinet, V. Georget, A. Lucas, E. Badia, A. Castet, R. White, J.-C. Nicolas, V. Cavailles, and S. Jalaguier Receptor-Interacting Protein 140 Is a Repressor of the Androgen Receptor Activity Mol. Endocrinol., July 1, 2006; 20(7): 1506 - 1518. [Abstract] [Full Text] [PDF]

				E. E. Dunham, E. A. Stevens, E. Glover, and C. A. Bradfield The Aryl Hydrocarbon Receptor Signaling Pathway Is Modified through Interactions with a Kelch Protein Mol. Pharmacol., July 1, 2006; 70(1): 8 - 15. [Abstract] [Full Text] [PDF]

				Y.-H. Chen, T. V. Beischlag, J. H. Kim, G. H. Perdew, and M. R. Stallcup Role of GAC63 in Transcriptional Activation Mediated by the Aryl Hydrocarbon Receptor J. Biol. Chem., May 5, 2006; 281(18): 12242 - 12247. [Abstract] [Full Text] [PDF]

				P. Augereau, E. Badia, M. Fuentes, F. Rabenoelina, M. Corniou, D. Derocq, P. Balaguer, and V. Cavailles Transcriptional Regulation of the Human NRIP1/RIP140 Gene by Estrogen Is Modulated by Dioxin Signalling Mol. Pharmacol., April 1, 2006; 69(4): 1338 - 1346. [Abstract] [Full Text] [PDF]

				J. Matthews, B. Wihlen, J. Thomsen, and J.-A. Gustafsson Aryl Hydrocarbon Receptor-Mediated Transcription: Ligand-Dependent Recruitment of Estrogen Receptor {alpha} to 2,3,7,8-Tetrachlorodibenzo- p-Dioxin-Responsive Promoters Mol. Cell. Biol., July 1, 2005; 25(13): 5317 - 5328. [Abstract] [Full Text] [PDF]

				T. V. Beischlag and G. H. Perdew ER{alpha}-AHR-ARNT Protein-Protein Interactions Mediate Estradiol-dependent Transrepression of Dioxin-inducible Gene Transcription J. Biol. Chem., June 3, 2005; 280(22): 21607 - 21611. [Abstract] [Full Text] [PDF]

				G. Huang and C. J. Elferink Multiple Mechanisms Are Involved in Ah Receptor-Mediated Cell Cycle Arrest Mol. Pharmacol., January 1, 2005; 67(1): 88 - 96. [Abstract] [Full Text] [PDF]

				T. V. Beischlag, R. T. Taylor, D. W. Rose, D. Yoon, Y. Chen, W.-H. Lee, M. G. Rosenfeld, and O. Hankinson Recruitment of Thyroid Hormone Receptor/Retinoblastoma-interacting Protein 230 by the Aryl Hydrocarbon Receptor Nuclear Translocator Is Required for the Transcriptional Response to Both Dioxin and Hypoxia J. Biol. Chem., December 24, 2004; 279(52): 54620 - 54628. [Abstract] [Full Text] [PDF]

				J. H. Kim and M. R. Stallcup Role of the Coiled-coil Coactivator (CoCoA) in Aryl Hydrocarbon Receptor-mediated Transcription J. Biol. Chem., November 26, 2004; 279(48): 49842 - 49848. [Abstract] [Full Text] [PDF]

				K. Ko, H. M. Theobald, R. W. Moore, and R. E. Peterson Evidence that Inhibited Prostatic Epithelial Bud Formation in 2,3,7,8-Tetrachlorodibenzo-p-dioxin-Exposed C57BL/6J Fetal Mice Is Not Due to Interruption of Androgen Signaling in the Urogenital Sinus Toxicol. Sci., June 1, 2004; 79(2): 360 - 369. [Abstract] [Full Text] [PDF]

				S. T. Pearce, H. Liu, I. Radhakrishnan, M. Abdelrahim, S. Safe, and V. C. Jordan Interaction of the Aryl Hydrocarbon Receptor Ligand 6-Methyl-1,3,8-trichlorodibenzofuran with Estrogen Receptor {alpha} Cancer Res., April 15, 2004; 64(8): 2889 - 2897. [Abstract] [Full Text] [PDF]

				A. Castet, A. Boulahtouf, G. Versini, S. Bonnet, P. Augereau, F. Vignon, S. Khochbin, S. Jalaguier, and V. Cavailles Multiple domains of the Receptor-Interacting Protein 140 contribute to transcription inhibition Nucleic Acids Res., April 1, 2004; 32(6): 1957 - 1966. [Abstract] [Full Text] [PDF]

				Y.-D. Wei, K. Tepperman, M.-y. Huang, M. A. Sartor, and A. Puga Chromium Inhibits Transcription from Polycyclic Aromatic Hydrocarbon-inducible Promoters by Blocking the Release of Histone Deacetylase and Preventing the Binding of p300 to Chromatin J. Biol. Chem., February 6, 2004; 279(6): 4110 - 4119. [Abstract] [Full Text] [PDF]

				A. Levine-Fridman, L. Chen, and C. J. Elferink Cytochrome P4501A1 Promotes G1 Phase Cell Cycle Progression by Controlling Aryl Hydrocarbon Receptor Activity Mol. Pharmacol., February 1, 2004; 65(2): 461 - 469. [Abstract] [Full Text] [PDF]

				E. V. Hestermann and M. Brown Agonist and Chemopreventative Ligands Induce Differential Transcriptional Cofactor Recruitment by Aryl Hydrocarbon Receptor Mol. Cell. Biol., November 1, 2003; 23(21): 7920 - 7925. [Abstract] [Full Text] [PDF]

				H. Tazawa, W. Osman, Y. Shoji, E. Treuter, J.-A. Gustafsson, and J. Zilliacus Regulation of Subnuclear Localization Is Associated with a Mechanism for Nuclear Receptor Corepression by RIP140 Mol. Cell. Biol., June 15, 2003; 23(12): 4187 - 4198. [Abstract] [Full Text] [PDF]

				M. Wormke, M. Stoner, B. Saville, K. Walker, M. Abdelrahim, R. Burghardt, and S. Safe The Aryl Hydrocarbon Receptor Mediates Degradation of Estrogen Receptor {alpha} through Activation of Proteasomes Mol. Cell. Biol., March 15, 2003; 23(6): 1843 - 1855. [Abstract] [Full Text] [PDF]

				T. V. Beischlag, S. Wang, D. W. Rose, J. Torchia, S. Reisz-Porszasz, K. Muhammad, W. E. Nelson, M. R. Probst, M. G. Rosenfeld, and O. Hankinson Recruitment of the NCoA/SRC-1/p160 Family of Transcriptional Coactivators by the Aryl Hydrocarbon Receptor/Aryl Hydrocarbon Receptor Nuclear Translocator Complex Mol. Cell. Biol., June 15, 2002; 22(12): 4319 - 4333. [Abstract] [Full Text] [PDF]

				J. M. Rogers and M. S. Denison Analysis of the Antiestrogenic Activity of 2,3,7,8-Tetrachlorodibenzo-p-dioxin in Human Ovarian Carcinoma BG-1 Cells Mol. Pharmacol., June 1, 2002; 61(6): 1393 - 1403. [Abstract] [Full Text] [PDF]

				S. Wang and O. Hankinson Functional Involvement of the Brahma/SWI2-related Gene 1 Protein in Cytochrome P4501A1 Transcription Mediated by the Aryl Hydrocarbon Receptor Complex J. Biol. Chem., March 29, 2002; 277(14): 11821 - 11827. [Abstract] [Full Text] [PDF]

				S. I. Karchner, D. G. Franks, W. H. Powell, and M. E. Hahn Regulatory Interactions among Three Members of the Vertebrate Aryl Hydrocarbon Receptor Family: AHR Repressor, AHR1, and AHR2 J. Biol. Chem., February 22, 2002; 277(9): 6949 - 6959. [Abstract] [Full Text] [PDF]

				J. C. Rowlands, L. He, R. Hakkak, M. J. J. Ronis, and T. M. Badger Soy and Whey Proteins Downregulate DMBA-Induced Liver and Mammary Gland CYP1 Expression in Female Rats J. Nutr., December 1, 2001; 131(12): 3281 - 3287. [Abstract] [Full Text] [PDF]

				M. B. Kumar, P. Ramadoss, R. K. Reen, J. P. Vanden Heuvel, and G. H. Perdew The Q-rich Subdomain of the Human Ah Receptor Transactivation Domain Is Required for Dioxin-mediated Transcriptional Activity J. Biol. Chem., November 2, 2001; 276(45): 42302 - 42310. [Abstract] [Full Text] [PDF]

				S. Nilsson, S. Makela, E. Treuter, M. Tujague, J. Thomsen, G. Andersson, E. Enmark, K. Pettersson, M. Warner, and J.-A. Gustafsson Mechanisms of Estrogen Action Physiol Rev, October 1, 2001; 81(4): 1535 - 1565. [Abstract] [Full Text] [PDF]

				J. Zilliacus, E. Holter, H. Wakui, H. Tazawa, E. Treuter, and J.-A. Gustafsson Regulation of Glucocorticoid Receptor Activity by 14-3-3-Dependent Intracellular Relocalization of the Corepressor RIP140 Mol. Endocrinol., April 1, 2001; 15(4): 501 - 511. [Abstract] [Full Text]

				C. J. Elferink, N.-L. Ge, and A. Levine Maximal Aryl Hydrocarbon Receptor Activity Depends on an Interaction with the Retinoblastoma Protein Mol. Pharmacol., April 1, 2001; 59(4): 664 - 673. [Abstract] [Full Text]

				S. M. Bello, D. G. Franks, J. J. Stegeman, and M. E. Hahn Acquired Resistance to Ah Receptor Agonists in a Population of Atlantic Killifish (Fundulus heteroclitus) Inhabiting a Marine Superfund Site: In Vivo and in Vitro Studies on the Inducibility of Xenobiotic Metabolizing Enzymes Toxicol. Sci., March 1, 2001; 60(1): 77 - 91. [Abstract] [Full Text] [PDF]

				D. L. Buchanan, T. Sato, R. E. Peterson, and P. S. Cooke Antiestrogenic Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin in Mouse Uterus: Critical Role of the Aryl Hydrocarbon Receptor in Stromal Tissue Toxicol. Sci., October 1, 2000; 57(2): 302 - 311. [Abstract] [Full Text] [PDF]

				S. A. Quadri, A. N. Qadri, M. E. Hahn, K. K. Mann, and D. H. Sherr The Bioflavonoid Galangin Blocks Aryl Hydrocarbon Receptor Activation and Polycyclic Aromatic Hydrocarbon-Induced Pre-B Cell Apoptosis Mol. Pharmacol., September 1, 2000; 58(3): 515 - 525. [Abstract] [Full Text]

				L.-N. Wei, M. Farooqui, and X. Hu Ligand-dependent Formation of Retinoid Receptors, Receptor-interacting Protein 140 (RIP140), and Histone Deacetylase Complex Is Mediated by a Novel Receptor-interacting Motif of RIP140 J. Biol. Chem., May 4, 2001; 276(19): 16107 - 16112. [Abstract] [Full Text] [PDF]