HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mak, D.-O. D. Articles by Foskett, J. K. Search for Related Content PubMed PubMed Citation Articles by Mak, D.-O. D. Articles by Foskett, J. K. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 32, 22231-22237, August 6, 1999

ATP Regulation of Type 1 Inositol 1,4,5-Trisphosphate Receptor Channel Gating by Allosteric Tuning of Ca²⁺ Activation

Don-On Daniel Mak, Sean McBride, and J. Kevin Foskett^§

From the  Department of Physiology and ^§ Institute for Human Gene Therapy, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6100

Inositol 1,4,5-trisphosphate (InsP₃) mobilizes intracellular Ca²⁺ by binding to its receptor (InsP₃R), an endoplasmic reticulum-localized Ca²⁺ release channel. Patch clamp electrophysiology of Xenopus oocyte nuclei was used to study the effects of cytoplasmic ATP concentration on the cytoplasmic Ca²⁺ ([Ca²⁺]_i) dependence of single type 1 InsP₃R channels in native endoplasmic reticulum membrane. Cytoplasmic ATP free-acid ([ATP]_i), but not the MgATP complex, activated gating of the InsP₃-liganded InsP₃R, by stabilizing open channel state(s) and destabilizing the closed state(s). Activation was associated with a reduction of the half-maximal activating [Ca²⁺]_i from 500 ± 50 nM in 0 [ATP]_i to 29 ± 4 nM in 9.5 mM [ATP]_i, with apparent ATP affinity = 0.27 ± 0.04 mM, similar to in vivo concentrations. In contrast, ATP was without effect on maximum open probability or the Hill coefficient for Ca²⁺ activation. Thus, ATP enhances gating of the InsP₃R by allosteric regulation of the Ca²⁺ sensitivity of the Ca²⁺ activation sites of the channel. By regulating the Ca²⁺-induced Ca²⁺ release properties of the InsP₃R, ATP may play an important role in shaping cytoplasmic Ca²⁺ signals, possibly linking cell metabolic state to important Ca²⁺-dependent processes.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				M. J. Betzenhauser, L. E. Wagner II, H. S. Park, and D. I. Yule ATP Regulation of Type-1 Inositol 1,4,5-Trisphosphate Receptor Activity Does Not Require Walker A-type ATP-binding Motifs J. Biol. Chem., June 12, 2009; 284(24): 16156 - 16163. [Abstract] [Full Text] [PDF]

				V. K. Nagaleekar, S. A. Diehl, I. Juncadella, C. Charland, N. Muthusamy, S. Eaton, L. Haynes, L. A. Garrett-Sinha, J. Anguita, and M. Rincon IP3 Receptor-Mediated Ca2+ Release in Naive CD4 T Cells Dictates Their Cytokine Program J. Immunol., December 15, 2008; 181(12): 8315 - 8322. [Abstract] [Full Text] [PDF]

				H. S. Park, M. J. Betzenhauser, J. H. Won, J. Chen, and D. I. Yule The Type 2 Inositol (1,4,5)-Trisphosphate (InsP3) Receptor Determines the Sensitivity of InsP3-induced Ca2+ Release to ATP in Pancreatic Acinar Cells J. Biol. Chem., September 19, 2008; 283(38): 26081 - 26088. [Abstract] [Full Text] [PDF]

				M. J. Betzenhauser, L. E. Wagner II, M. Iwai, T. Michikawa, K. Mikoshiba, and D. I. Yule ATP Modulation of Ca2+ Release by Type-2 and Type-3 Inositol (1, 4, 5)-Triphosphate Receptors: DIFFERING ATP SENSITIVITIES AND MOLECULAR DETERMINANTS OF ACTION J. Biol. Chem., August 1, 2008; 283(31): 21579 - 21587. [Abstract] [Full Text] [PDF]

				L. E. Wagner II, S. K. Joseph, and D. I. Yule Regulation of single inositol 1,4,5-trisphosphate receptor channel activity by protein kinase A phosphorylation J. Physiol., August 1, 2008; 586(15): 3577 - 3596. [Abstract] [Full Text] [PDF]

				J. M. Power and P. Sah Competition between Calcium-Activated K+ Channels Determines Cholinergic Action on Firing Properties of Basolateral Amygdala Projection Neurons J. Neurosci., March 19, 2008; 28(12): 3209 - 3220. [Abstract] [Full Text] [PDF]

				J. K. Foskett, C. White, K.-H. Cheung, and D.-O. D. Mak Inositol Trisphosphate Receptor Ca2+ Release Channels Physiol Rev, April 1, 2007; 87(2): 593 - 658. [Abstract] [Full Text] [PDF]

				C. White, J. Yang, M. J. Monteiro, and J. K. Foskett CIB1, a Ubiquitously Expressed Ca2+-binding Protein Ligand of the InsP3 Receptor Ca2+ Release Channel J. Biol. Chem., July 28, 2006; 281(30): 20825 - 20833. [Abstract] [Full Text] [PDF]

				L. E. Wagner II, M. J. Betzenhauser, and D. I. Yule ATP Binding to a Unique Site in the Type-1 S2- Inositol 1,4,5-Trisphosphate Receptor Defines Susceptibility to Phosphorylation by Protein Kinase A J. Biol. Chem., June 23, 2006; 281(25): 17410 - 17419. [Abstract] [Full Text] [PDF]

				F.-X. Yi and I. M. Bird Pregnancy-Specific Modulatory Role of Mitochondria on Adenosine 5'-Triphosphate-Induced Cytosolic [Ca2+] Signaling in Uterine Artery Endothelial Cells Endocrinology, November 1, 2005; 146(11): 4844 - 4850. [Abstract] [Full Text] [PDF]

				R. Dumollard, P. Marangos, G. Fitzharris, K. Swann, M. Duchen, and J. Carroll Sperm-triggered [Ca2+] oscillations and Ca2+ homeostasis in the mouse egg have an absolute requirement for mitochondrial ATP production Development, July 1, 2004; 131(13): 3057 - 3067. [Abstract] [Full Text] [PDF]

				D.-O. D. Mak, S. M.J. McBride, and J. K. Foskett Spontaneous Channel Activity of the Inositol 1,4,5-Trisphosphate (InsP3) Receptor (InsP3R). Application of Allosteric Modeling to Calcium and InsP3 Regulation of InsP3R Single-channel Gating J. Gen. Physiol., October 27, 2003; 122(5): 583 - 603. [Abstract] [Full Text] [PDF]

				R. Dumollard, K. Hammar, M. Porterfield, P. J. Smith, C. Cibert, C. Rouviere, and C. Sardet Mitochondrial respiration and Ca2+ waves are linked during fertilization and meiosis completion Development, February 15, 2003; 130(4): 683 - 692. [Abstract] [Full Text] [PDF]

				J. C. Lenz, H. P. Reusch, N. Albrecht, G. Schultz, and M. Schaefer Ca2+-controlled competitive diacylglycerol binding of protein kinase C isoenzymes in living cells J. Cell Biol., October 28, 2002; 159(2): 291 - 302. [Abstract] [Full Text] [PDF]

				R. Dumollard, J. Carroll, G. Dupont, and C. Sardet Calcium wave pacemakers in eggs J. Cell Sci., September 15, 2002; 115(18): 3557 - 3564. [Abstract] [Full Text] [PDF]

				S. H. Yoo, Y. S. Oh, M. K. Kang, Y. H. Huh, S. H. So, H. S. Park, and H. Y. Park Localization of Three Types of the Inositol 1,4,5-Trisphosphate Receptor/Ca2+ Channel in the Secretory Granules and Coupling with the Ca2+ Storage Proteins Chromogranins A and B J. Biol. Chem., November 30, 2001; 276(49): 45806 - 45812. [Abstract] [Full Text] [PDF]

				L. Missiaen, H. DeSmedt, G. Bultynck, S. Vanlingen, P. Desmet, G. Callewaert, and J. B. Parys Calmodulin Increases the Sensitivity of Type 3 Inositol-1,4,5-trisphosphate Receptors to Ca2+ Inhibition in Human Bronchial Mucosal Cells Mol. Pharmacol., March 1, 2000; 57(3): 564 - 567. [Abstract] [Full Text]

				D. Boehning and S. K. Joseph Functional Properties of Recombinant Type I and Type III Inositol 1,4,5-Trisphosphate Receptor Isoforms Expressed in COS-7 Cells J. Biol. Chem., July 7, 2000; 275(28): 21492 - 21499. [Abstract] [Full Text] [PDF]

				J. S. Marchant, V. Ramos, and I. Parker Structural and functional relationships between Ca2+ puffs and mitochondria in Xenopus oocytes Am J Physiol Cell Physiol, June 1, 2002; 282(6): C1374 - C1386. [Abstract] [Full Text] [PDF]