HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Skinner, M. A. Articles by Wildeman, A. G. Search for Related Content PubMed PubMed Citation Articles by Skinner, M. A. Articles by Wildeman, A. G. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 33, 23119-23127, August 13, 1999

₁ Integrin Binds the 16-kDa Subunit of Vacuolar H⁺-ATPase at a Site Important for Human Papillomavirus E5 and Platelet-derived Growth Factor Signaling

From the Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario N1G 2W1, Canada

Integrins mediate adhesive interactions between cells and the extracellular matrix, and play a role in cell migration, proliferation, differentiation, cytoskeletal organization, and signal transduction. We have identified an interaction between the ₁ integrin and the 16-kDa subunit of vacuolar H⁺-ATPase (16K). This interaction was first isolated in a yeast two-hybrid screen and confirmed by coimmunoprecipitation and in in vitro binding assays using bacterially expressed proteins. Immunofluorescent studies performed in L6 myoblasts expressing both native and epitope-tagged 16K demonstrate co-localization with ₁ integrin in focal adhesions. Deletion of the fourth of four transmembrane helices in 16K results in loss of interaction with ₁ integrin in vitro and in the two-hybrid system, and less prominent staining in focal adhesions. This helix is also required for ligand-independent activation of platelet-derived growth factor- receptor signaling by the human papillomavirus E5 oncoprotein. Overexpression of 16K or expression of 16K lacking this helix alters the morphology of myoblasts and fibroblasts, suggesting that the interaction of 16K with integrins could be important for cell growth control. We also discuss the possible role 16K might play in integrin movement.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				X. Lu, W. Qin, J. Li, N. Tan, D. Pan, H. Zhang, L. Xie, G. Yao, H. Shu, M. Yao, et al. The Growth and Metastasis of Human Hepatocellular Carcinoma Xenografts Are Inhibited by Small Interfering RNA Targeting to the Subunit ATP6L of Proton Pump Cancer Res., August 1, 2005; 65(15): 6843 - 6849. [Abstract] [Full Text] [PDF]

				I. Lee, M. A. Skinner, H.-b. Guo, A. Sujan, and M. Pierce Expression of the Vacuolar H+-ATPase 16-kDa Subunit Results in the Triton X-100-insoluble Aggregation of {beta}1 Integrin and Reduction of Its Cell Surface Expression J. Biol. Chem., December 17, 2004; 279(51): 53007 - 53014. [Abstract] [Full Text] [PDF]

				C. A. Wagner, K. E. Finberg, S. Breton, V. Marshansky, D. Brown, and J. P. Geibel Renal Vacuolar H+-ATPase Physiol Rev, October 1, 2004; 84(4): 1263 - 1314. [Abstract] [Full Text] [PDF]

				A.-Q. Sun, N. Balasubramaniyan, C.-J. Liu, M. Shahid, and F. J. Suchy Association of the 16-kDa Subunit c of Vacuolar Proton Pump with the Ileal Na+-dependent Bile Acid Transporter: PROTEIN-PROTEIN INTERACTION AND INTRACELLULAR TRAFFICKING J. Biol. Chem., April 16, 2004; 279(16): 16295 - 16300. [Abstract] [Full Text] [PDF]

				M. Lu, S. Vergara, L. Zhang, L. S. Holliday, J. Aris, and S. L. Gluck The Amino-terminal Domain of the E Subunit of Vacuolar H+-ATPase (V-ATPase) Interacts with the H Subunit and Is Required for V-ATPase Function J. Biol. Chem., October 4, 2002; 277(41): 38409 - 38415. [Abstract] [Full Text] [PDF]

				T. Torigoe, H. Izumi, H. Ishiguchi, H. Uramoto, T. Murakami, T. Ise, Y. Yoshida, M. Tanabe, M. Nomoto, H. Itoh, et al. Enhanced Expression of the Human Vacuolar H+-ATPase c subunit Gene (ATP6L) in Response to Anticancer Agents J. Biol. Chem., September 20, 2002; 277(39): 36534 - 36543. [Abstract] [Full Text] [PDF]

				M. A. Skinner and A. G. Wildeman Suppression of Tumor-related Glycosylation of Cell Surface Receptors by the 16-kDa Membrane Subunit of Vacuolar H+-ATPase J. Biol. Chem., December 14, 2001; 276(51): 48451 - 48457. [Abstract] [Full Text] [PDF]

				M. Lu, L. S. Holliday, L. Zhang, W. A. Dunn Jr., and S. L. Gluck Interaction between Aldolase and Vacuolar H+-ATPase. EVIDENCE FOR DIRECT COUPLING OF GLYCOLYSIS TO THE ATP-HYDROLYZING PROTON PUMP J. Biol. Chem., August 3, 2001; 276(32): 30407 - 30413. [Abstract] [Full Text] [PDF]

				T. Nishi, S. Kawasaki-Nishi, and M. Forgac Expression and Localization of the Mouse Homologue of the Yeast V-ATPase 21-kDa Subunit c'' (Vma16p) J. Biol. Chem., August 31, 2001; 276(36): 34122 - 34130. [Abstract] [Full Text] [PDF]