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J Biol Chem, Vol. 274, Issue 34, 23691-23694, August 20, 1999
From the Department of Biochemistry and Molecular Biology,
Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada
In trypanosomatid protozoa, all mRNAs obtain
identical 5'-ends by trans-splicing of the 5'-terminal 39 nucleotides of a small spliced leader RNA to appropriate acceptor sites
in pre-mRNA. Although this process involves spliceosomal small
nuclear (sn) RNAs, it is thought that trypanosomatids do not contain a
homolog of the cis-spliceosomal U1 snRNA. We show here that
a trypanosomatid protozoon, Crithidia fasciculata, contains
a novel small RNA that displays several features characteristic of a U1
snRNA, including (i) a methylguanosine cap and additional 5'-terminal
modifications, (ii) a potential binding site for common core proteins
that are present in other trans-spliceosomal
ribonucleoproteins, (iii) a U1-like 5'-terminal sequence, and (iv) a
U1-like stem/loop I structure. Because trypanosomatid pre-mRNAs do
not appear to contain cis-spliced introns, we argue that
this previously unrecognized RNA species is a good candidate to be a
trans-spliceosomal U1 snRNA.
This article has been cited by other articles:
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X.-h. Liang, A. Haritan, S. Uliel, and S. Michaeli trans and cis Splicing in Trypanosomatids: Mechanism, Factors, and Regulation Eukaryot. Cell, October 1, 2003; 2(5): 830 - 840. [Full Text] [PDF]
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Y.-i. Watanabe and M. W. Gray Evolutionary appearance of genes encoding proteins associated with box H/ACA snoRNAs: Cbf5p in Euglena gracilis, an early diverging eukaryote, and candidate Gar1p and Nop10p homologs in archaebacteria Nucleic Acids Res., June 15, 2000; 28(12): 2342 - 2352. [Abstract] [Full Text] [PDF]
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