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J Biol Chem, Vol. 274, Issue 34, 23691-23694, August 20, 1999
COMMUNICATION
A Candidate U1 Small Nuclear RNA for Trypanosomatid Protozoa
Murray N.
Schnare and
Michael W.
Gray
From the Department of Biochemistry and Molecular Biology,
Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada
In trypanosomatid protozoa, all mRNAs obtain
identical 5'-ends by trans-splicing of the 5'-terminal 39 nucleotides of a small spliced leader RNA to appropriate acceptor sites
in pre-mRNA. Although this process involves spliceosomal small
nuclear (sn) RNAs, it is thought that trypanosomatids do not contain a
homolog of the cis-spliceosomal U1 snRNA. We show here that
a trypanosomatid protozoon, Crithidia fasciculata, contains
a novel small RNA that displays several features characteristic of a U1
snRNA, including (i) a methylguanosine cap and additional 5'-terminal
modifications, (ii) a potential binding site for common core proteins
that are present in other trans-spliceosomal
ribonucleoproteins, (iii) a U1-like 5'-terminal sequence, and (iv) a
U1-like stem/loop I structure. Because trypanosomatid pre-mRNAs do
not appear to contain cis-spliced introns, we argue that
this previously unrecognized RNA species is a good candidate to be a
trans-spliceosomal U1 snRNA.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.

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Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.
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