| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 34, 23702-23706, August 20, 1999
From the Endocrine Research Unit, Mayo Clinic,
Rochester, Minnesota 55905
The utilization of blood glycerol and glucose as
precursors for intramuscular triglyceride synthesis was examined in
rats using an intravenous infusion of
[2-14C]glycerol and [6-3H]glucose or
[6-14C]glucose. In 24-h fasted rats, more glycerol than
glucose was incorporated into intramuscular triglyceride glycerol in
soleus (69 ± 23 versus 4 ± 1 nmol/µmol
triglyceride/h, respectively, p = 0.02 glycerol
versus glucose) and in gastrocnemius (25 ± 5 versus 9 ± 2 nmol/µmol triglyceride/h,
respectively, p = 0.02). Blood glucose was utilized
more than blood glycerol for triglyceride glycerol synthesis in
quadriceps. In fed rats, the blood glycerol incorporation rates (4 ± 2, 8 ± 3, and 9 ± 3 nmol/µmol triglyceride/h) were
similar (p > 0.3) to those of glucose (5 ± 2, 8 ± 2, and 5 ± 2 nmol/µmol triglyceride/h for quadriceps,
gastrocnemius, and soleus muscle, respectively). Glucose incorporation
into intramuscular triglycerides was less with
[6-3H]glucose than with [6-14C]glucose,
suggesting an indirect pathway for glucose carbon entry into muscle
triglyceride. The isotopic equilibrium between plasma and intramuscular
free glycerol ([U-13C]glycerol) was complete in
quadriceps and gastrocnemius, but not soleus, within 2 h after
beginning the tracer infusion. We conclude that blood glycerol is a
direct and important precursor for muscle triglyceride synthesis in
rats, confirming the presence of functionally important amounts of
glycerol kinase in skeletal muscle.
This article has been cited by other articles:
|
|
 |
|
  C. Moro, S. Bajpeyi, and S. R. Smith Determinants of intramyocellular triglyceride turnover: implications for insulin sensitivity Am J Physiol Endocrinol Metab, February 1, 2008; 294(2): E203 - E213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Wallis, A. L. Friedlander, K. A. Jacobs, M. A. Horning, J. A. Fattor, E. E. Wolfel, G. D. Lopaschuk, and G. A. Brooks Substantial working muscle glycerol turnover during two-legged cycle ergometry Am J Physiol Endocrinol Metab, October 1, 2007; 293(4): E950 - E957. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Guo, L. Zhou, and M. D. Jensen Acute hyperinsulinemia inhibits intramyocellular triglyceride synthesis in high-fat-fed obese rats J. Lipid Res., December 1, 2006; 47(12): 2640 - 2646. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. P. Kinman, T. Kasumov, K. A. Jobbins, K. R. Thomas, J. E. Adams, L. N. Brunengraber, G. Kutz, W.-U. Brewer, C. R. Roe, and H. Brunengraber Parenteral and enteral metabolism of anaplerotic triheptanoin in normal rats Am J Physiol Endocrinol Metab, October 1, 2006; 291(4): E860 - E866. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Horton, E. K. Miller, and K. Bourret No effect of menstrual cycle phase on glycerol or palmitate kinetics during 90 min of moderate exercise J Appl Physiol, March 1, 2006; 100(3): 917 - 925. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Qvisth, E. Hagstrom-Toft, S. Enoksson, E. Moberg, P. Arner, and J. Bolinder Human Skeletal Muscle Lipolysis Is More Responsive to Epinephrine Than to Norepinephrine Stimulation in Vivo J. Clin. Endocrinol. Metab., February 1, 2006; 91(2): 665 - 670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-S. Cha, T. P. Ciaraldi, K.-S. Park, L. Carter, S. R. Mudaliar, and R. R. Henry Impaired fatty acid metabolism in type 2 diabetic skeletal muscle cells is reversed by PPAR{gamma} agonists Am J Physiol Endocrinol Metab, July 1, 2005; 289(1): E151 - E159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Enoksson, E. Hagstrom-Toft, J. Nordahl, K. Hultenby, N. Pettersson, B. Isaksson, J. Permert, R. Wibom, C. Holm, J. Bolinder, et al. Marked Reutilization of Free Fatty Acids during Activated Lipolysis in Human Skeletal Muscle J. Clin. Endocrinol. Metab., February 1, 2005; 90(2): 1189 - 1195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sacchetti, B. Saltin, D. B Olsen, and G. van Hall High triacylglycerol turnover rate in human skeletal muscle J. Physiol., December 15, 2004; 561(3): 883 - 891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. W. Zderic, S. Schenk, C. J. Davidson, L. O. Byerley, and E. F. Coyle Manipulation of dietary carbohydrate and muscle glycogen affects glucose uptake during exercise when fat oxidation is impaired by {beta}-adrenergic blockade Am J Physiol Endocrinol Metab, December 1, 2004; 287(6): E1195 - E1201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Qvisth, E. Hagstrom-Toft, S. Enoksson, R. S. Sherwin, S. Sjoberg, and J. Bolinder Combined Hyperinsulinemia and Hyperglycemia, But Not Hyperinsulinemia Alone, Suppress Human Skeletal Muscle Lipolytic Activity in Vivo J. Clin. Endocrinol. Metab., September 1, 2004; 89(9): 4693 - 4700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. W. Zderic, C. J. Davidson, S. Schenk, L. O. Byerley, and E. F. Coyle High-fat diet elevates resting intramuscular triglyceride concentration and whole body lipolysis during exercise Am J Physiol Endocrinol Metab, February 1, 2004; 286(2): E217 - E225. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Reshef, Y. Olswang, H. Cassuto, B. Blum, C. M. Croniger, S. C. Kalhan, S. M. Tilghman, and R. W. Hanson Glyceroneogenesis and the Triglyceride/Fatty Acid Cycle J. Biol. Chem., August 15, 2003; 278(33): 30413 - 30416. [Full Text] [PDF]
|
|
|
|
 |
|
 W. T. L. Festuccia, R. Guerra-Sa, N. H. Kawashita, M. A. R. Garofalo, E. A. Evangelista, V. Rodrigues, I. C. Kettelhut, and R. H. Migliorini Expression of glycerokinase in brown adipose tissue is stimulated by the sympathetic nervous system Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2003; 284(6): R1536 - R1541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Moberg, S. Sjoberg, E. Hagstrom-Toft, and J. Bolinder No apparent suppression by insulin of in vivo skeletal muscle lipolysis in nonobese women Am J Physiol Endocrinol Metab, August 1, 2002; 283(2): E295 - E301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sjostrand, S. Gudbjornsdottir, A. Holmang, L. Strindberg, K. Ekberg, and P. Lonnroth Measurements of Interstitial Muscle Glycerol in Normal and Insulin-Resistant Subjects J. Clin. Endocrinol. Metab., May 1, 2002; 87(5): 2206 - 2211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. H. Kawashita, W. T. L. Festuccia, M. N. Brito, M. A. F. Moura, S. R. C. Brito, M. A. R. Garofalo, I. C. Kettelhut, and R. H. Migliorini Glycerokinase activity in brown adipose tissue: a sympathetic regulation? Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2002; 282(4): R1185 - R1190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. P. Lee, A. S. Deonarine, M. Kienetz, Q. Zhu, M. Skrzypczak, M. Chan, and P. C. Choy A novel pathway for lipid biosynthesis: the direct acylation of glycerol J. Lipid Res., December 1, 2001; 42(12): 1979 - 1986. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Voshol, M. C. Jong, V. E.H. Dahlmans, D. Kratky, S. Levak-Frank, R. Zechner, J. A. Romijn, and L. M. Havekes In Muscle-Specific Lipoprotein Lipase-Overexpressing Mice, Muscle Triglyceride Content Is Increased Without Inhibition of Insulin-Stimulated Whole-Body and Muscle-Specific Glucose Uptake Diabetes, November 1, 2001; 50(11): 2585 - 2590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Carter, C. Rennie, and M. A. Tarnopolsky Substrate utilization during endurance exercise in men and women after endurance training Am J Physiol Endocrinol Metab, June 1, 2001; 280(6): E898 - E907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Burelle, D. Massicotte, M. Lussier, C. Lavoie, C. Hillaire-Marcel, and F. Peronnet Oxidation of [13C]glycerol ingested along with glucose during prolonged exercise J Appl Physiol, May 1, 2001; 90(5): 1685 - 1690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. F. Coyle, A. E. Jeukendrup, M. C. Oseto, B. J. Hodgkinson, and T. W. Zderic Low-fat diet alters intramuscular substrates and reduces lipolysis and fat oxidation during exercise Am J Physiol Endocrinol Metab, March 1, 2001; 280(3): E391 - E398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Montell, C. Lerin, C. B. Newgard, and A. M. Gomez-Foix Effects of Modulation of Glycerol Kinase Expression on Lipid and Carbohydrate Metabolism in Human Muscle Cells J. Biol. Chem., January 18, 2002; 277(4): 2682 - 2686. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
