| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 34, 23969-23976, August 20, 1999
From the Department of Pharmacology, Medical School, University of
Patras, Patras 261 10, Greece
Many of the cellular actions of thrombin may
contribute to the angiogenesis-promoting effect of thrombin reported
previously. In this study, we investigated the interaction between
thrombin and vascular endothelial growth factor (VEGF), the specific
endothelial cell mitogen and key angiogenic factor. Exposure of human
umbilical vein endothelial cells to thrombin sensitizes these cells to
the mitogenic activity of VEGF. This thrombin-mediated effect is
specific, dose-dependent and requires the activated
thrombin receptor. Quantitative reverse transcription- polymerase chain
reaction analysis reveals a time- and dose-dependent
up-regulation of mRNA for VEGF receptors (KDR and flt-1). Optimal
thrombin concentration for maximal expression of mRNA for KDR is
1.5 IU/ml (170% over controls) and appears 8-12 h after thrombin
stimulation. Nuclear run-on experiments demonstrate that the
up-regulation of KDR mRNA by thrombin occurred at the
transcriptional level. In addition, functional protein of KDR receptor
is increased to about 200% over control after 12 h of thrombin
treatment. The up-regulation of KDR and flt-1 mRNA is also mimicked
by the thrombin receptor activating peptide. These findings could
explain at least in part the potent angiogenic action of thrombin.
This article has been cited by other articles:
|
|
 |
|
  P. Zania, M. Papaconstantinou, C. S. Flordellis, M. E. Maragoudakis, and N. E. Tsopanoglou Thrombin mediates mitogenesis and survival of human endothelial cells through distinct mechanisms Am J Physiol Cell Physiol, May 1, 2008; 294(5): C1215 - C1226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hoffman, A. Harger, A. Lenkowski, U. Hedner, H. R. Roberts, and D. M. Monroe Cutaneous wound healing is impaired in hemophilia B Blood, November 1, 2006; 108(9): 3053 - 3060. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Zania, S. Kritikou, C. S. Flordellis, M. E. Maragoudakis, and N. E. Tsopanoglou Blockade of Angiogenesis by Small Molecule Antagonists to Protease-Activated Receptor-1: Association with Endothelial Cell Growth Suppression and Induction of Apoptosis J. Pharmacol. Exp. Ther., July 1, 2006; 318(1): 246 - 254. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Lin, A. Hamik, R. Jain, A. Kumar, and M. K. Jain Kruppel-Like Factor 2 Inhibits Protease Activated Receptor-1 Expression and Thrombin-Mediated Endothelial Activation Arterioscler. Thromb. Vasc. Biol., May 1, 2006; 26(5): 1185 - 1185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Caunt, L. Hu, T. Tang, P. C. Brooks, S. Ibrahim, and S. Karpatkin Growth-regulated oncogene is pivotal in thrombin-induced angiogenesis. Cancer Res., April 15, 2006; 66(8): 4125 - 4132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Smadja, I. Bieche, G. Uzan, H. Bompais, L. Muller, C. Boisson-Vidal, M. Vidaud, M. Aiach, and P. Gaussem PAR-1 Activation on Human Late Endothelial Progenitor Cells Enhances Angiogenesis In Vitro With Upregulation of the SDF-1/CXCR4 System Arterioscler. Thromb. Vasc. Biol., November 1, 2005; 25(11): 2321 - 2327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Naldini, C. Bernini, A. Pucci, and F. Carraro Thrombin-mediated IL-10 up-regulation involves protease-activated receptor (PAR)-1 expression in human mononuclear leukocytes J. Leukoc. Biol., September 1, 2005; 78(3): 736 - 744. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Belting, J. Ahamed, and W. Ruf Signaling of the Tissue Factor Coagulation Pathway in Angiogenesis and Cancer Arterioscler. Thromb. Vasc. Biol., August 1, 2005; 25(8): 1545 - 1550. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Chang, R. Yamada, T. Sawada, A. Suzuki, Y. Kochi, and K. Yamamoto The inhibition of antithrombin by peptidylarginine deiminase 4 may contribute to pathogenesis of rheumatoid arthritis Rheumatology, March 1, 2005; 44(3): 293 - 298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Fareed, O. Iqbal, M. Tobu, D. A. Hoppensteadt, and J. Fareed Blood Levels of Nitric Oxide, C-Reactive Protein, and Tumor Necrosis Factor-{alpha} Are Upregulated in Patients with Malignancy-Associated Hypercoagulable State: Pathophysiologic Implications Clinical and Applied Thrombosis/Hemostasis, October 1, 2004; 10(4): 357 - 364. [Abstract] [PDF]
|
|
|
|
 |
|
 J. C. Mason, R. Steinberg, E. A. Lidington, A. R. Kinderlerer, M. Ohba, and D. O. Haskard Decay-accelerating Factor Induction on Vascular Endothelium by Vascular Endothelial Growth Factor (VEGF) Is Mediated via a VEGF Receptor-2 (VEGF-R2)- and Protein Kinase C-{alpha}/{epsilon} (PKC{alpha}/{epsilon})-dependent Cytoprotective Signaling Pathway and Is Inhibited by Cyclosporin A J. Biol. Chem., October 1, 2004; 279(40): 41611 - 41618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.H. Tayebjee, G.Y.H. Lip, and R.J. MacFadyen Collateralization and the response to obstruction of epicardial coronary arteries QJM, May 1, 2004; 97(5): 259 - 272. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. S. OSSOVSKAYA and N. W. BUNNETT Protease-Activated Receptors: Contribution to Physiology and Disease Physiol Rev, April 1, 2004; 84(2): 579 - 621. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. J. Davie, J. T. Crossno Jr., M. G. Frid, S. E. Hofmeister, J. T. Reeves, D. M. Hyde, T. C. Carpenter, J. A. Brunetti, I. K. McNiece, and K. R. Stenmark Hypoxia-induced pulmonary artery adventitial remodeling and neovascularization: contribution of progenitor cells Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L668 - L678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Lockwood, P. Kumar, G. Krikun, S. Kadner, P. Dubon, H. Critchley, and F. Schatz Effects of Thrombin, Hypoxia, and Steroids on Interleukin-8 Expression in Decidualized Human Endometrial Stromal Cells: Implications for Long-Term Progestin-Only Contraceptive-Induced Bleeding J. Clin. Endocrinol. Metab., March 1, 2004; 89(3): 1467 - 1475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Minami, A. Sugiyama, S.-Q. Wu, R. Abid, T. Kodama, and W. C. Aird Thrombin and Phenotypic Modulation of the Endothelium Arterioscler. Thromb. Vasc. Biol., January 1, 2004; 24(1): 41 - 53. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Daly, A. Makris, M. Reed, and C. E. Lewis Hemostatic Regulators of Tumor Angiogenesis: A Source of Antiangiogenic Agents for Cancer Treatment? J Natl Cancer Inst, November 19, 2003; 95(22): 1660 - 1673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kataoka, J. R. Hamilton, D. D. McKemy, E. Camerer, Y.-W. Zheng, A. Cheng, C. Griffin, and S. R. Coughlin Protease-activated receptors 1 and 4 mediate thrombin signaling in endothelial cells Blood, November 1, 2003; 102(9): 3224 - 3231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Ardissino, P. A. Merlini, K. A. Bauer, M. Galvani, F. Ottani, F. Franchi, F. Bertocchi, R. D. Rosenberg, and P. M. Mannucci Coagulation activation and long-term outcome in acute coronary syndromes Blood, October 15, 2003; 102(8): 2731 - 2735. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. G. Mann Thrombin: Can't Live Without It; Probably Die From It Chest, September 1, 2003; 124(3_suppl): 1S - 3S. [Full Text] [PDF]
|
|
|
|
|
|
C. D. Major, R. J. Santulli, C. K. Derian, and P. Andrade-Gordon Extracellular Mediators in Atherosclerosis and Thrombosis: Lessons From Thrombin Receptor Knockout Mice Arterioscler. Thromb. Vasc. Biol., June 1, 2003; 23(6): 931 - 939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Moser and C. Patterson Thrombin and Vascular Development: A Sticky Subject Arterioscler. Thromb. Vasc. Biol., June 1, 2003; 23(6): 922 - 930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. C. Aird The role of the endothelium in severe sepsis and multiple organ dysfunction syndrome Blood, May 15, 2003; 101(10): 3765 - 3777. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. Manegold, J. Hutter, S. A. Pahernik, K. Messmer, and M. Dellian Platelet-endothelial interaction in tumor angiogenesis and microcirculation Blood, March 1, 2003; 101(5): 1970 - 1976. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-J. YIN, Z. SALAH, M. MAOZ, S. C. E. RAM, S. OCHAYON, G. NEUFELD, S. KATZAV, and R. BAR-SHAVIT Oncogenic transformation induces tumor angiogenesis: a role for PAR1 activation FASEB J, February 1, 2003; 17(2): 163 - 174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-Q. Wu, T. Minami, D. J. Donovan, and W. C. Aird The proximal serum response element in the Egr-1 promoter mediates response to thrombin in primary human endothelial cells Blood, December 15, 2002; 100(13): 4454 - 4461. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. E. Tsopanoglou, P. Andriopoulou, and M. E. Maragoudakis On the mechanism of thrombin-induced angiogenesis: involvement of alpha vbeta 3-integrin Am J Physiol Cell Physiol, November 1, 2002; 283(5): C1501 - C1510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Lockwood, G. Krikun, A. B. C. Koo, S. Kadner, and F. Schatz Differential Effects of Thrombin and Hypoxia on Endometrial Stromal and Glandular Epithelial Cell Vascular Endothelial Growth Factor Expression J. Clin. Endocrinol. Metab., September 1, 2002; 87(9): 4280 - 4286. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Vouret-Craviari, C. Bourcier, E. Boulter, and E. Van Obberghen-Schilling Distinct signals via Rho GTPases and Src drive shape changes by thrombin and sphingosine-1-phosphate in endothelial cells J. Cell Sci., June 15, 2002; 115(12): 2475 - 2484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-Q. Huang, J.-J. Li, L. Hu, M. Lee, and S. Karpatkin Thrombin induces increased expression and secretion of angiopoietin-2 from human umbilical vein endothelial cells Blood, March 1, 2002; 99(5): 1646 - 1650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. T. Griffin, Y. Srinivasan, Y.-W. Zheng, W. Huang, and S. R. Coughlin A Role for Thrombin Receptor Signaling in Endothelial Cells During Embryonic Development Science, August 31, 2001; 293(5535): 1666 - 1670. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Patterson, G. A. Stouffer, N. Madamanchi, and M. S. Runge New Tricks for Old Dogs : Nonthrombotic Effects of Thrombin in Vessel Wall Biology Circ. Res., May 25, 2001; 88(10): 987 - 997. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bhattacharya, G. F. Smith, and M. L. Cohen Effect of LY287045, a Thrombin/Trypsin Inhibitor, on Thrombin and Trypsin-Induced Aortic Contraction and Relaxation J. Pharmacol. Exp. Ther., April 12, 2001; 297(2): 573 - 581. [Abstract] [Full Text]
|
|
|
|
|
|
E. A. Lidington, D. O. Haskard, and J. C. Mason Induction of decay-accelerating factor by thrombin through a protease-activated receptor 1 and protein kinase C-dependent pathway protects vascular endothelial cells from complement-mediated injury Blood, October 15, 2000; 96(8): 2784 - 2792. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Bhattacharya Gene Therapy for Pulmonary Edema Am. J. Respir. Cell Mol. Biol., June 1, 2000; 22(6): 640 - 641. [Full Text]
|
|
|
|
|
|
A. Gilchrist, J. F. Vanhauwe, A. Li, T. O. Thomas, T. Voyno-Yasenetskaya, and H. E. Hamm Galpha Minigenes Expressing C-terminal Peptides Serve as Specific Inhibitors of Thrombin-mediated Endothelial Activation J. Biol. Chem., July 6, 2001; 276(28): 25672 - 25679. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
