JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liu, X.
Right arrow Articles by Ambudkar, I. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, X.
Right arrow Articles by Ambudkar, I. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 274, Issue 35, 25121-25129, August 27, 1999

ATP-dependent Activation of KCa and ROMK-type KATP Channels in Human Submandibular Gland Ductal Cells

Xibao Liu, Brij B. Singh, and Indu S. Ambudkar

From the Secretory Physiology Section, Gene Therapy and Therapeutics Branch, NIDCR, National Institutes of Health, Bethesda, Maryland 20892

[Ca2+]i and membrane current were measured in human submandibular gland ductal (HSG) cells to determine the regulation of salivary cell function by ATP. 1-10 µM ATP activated internal Ca2+ release, outward Ca2+-dependent K+ channel (KCa), and inward store-operated Ca2+ current (ISOC). The subsequent addition of 100 µM ATP activated an inwardly rectifying K+ current, without increasing [Ca2+]i. The K+ current was also stimulated by ATP in cells treated with thapsigargin in a Ca2+-free medium and was blocked by glibenclamide and tolbutamide, but not by charybdotoxin. This suggests the involvement of a Ca2+-independent, sulfonylurea-sensitive K+ channel (KATP). UTP mimicked the low [ATP] effects, while benzoyl-ATP activated internal Ca2+ release, a Ca2+ influx pathway, and KCa. Thus, ATP acts via P2U (P2Y2) and P2Z (P2X7) receptors to increase [Ca2+]i and activate KCa, but not KATP. Importantly, (i) ROMK1 and the cystic fibrosis transmembrane regulator protein (but not SUR1, SUR2A, or SUR2B) and (ii) cAMP-stimulated Cl- and K+ currents were detected in HSG cells. These data demonstrate for the first time that a ROMK-type KATP channel is present in salivary gland duct cells that is regulated by extracellular ATP and possibly by the cystic fibrosis transmembrane regulator. This reveals a potentially novel mechanism for K+ secretion in these cells.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
T. Nakamoto, V. G. Romanenko, A. Takahashi, T. Begenisich, and J. E. Melvin
Apical maxi-K (KCa1.1) channels mediate K+ secretion by the mouse submandibular exocrine gland
Am J Physiol Cell Physiol, March 1, 2008; 294(3): C810 - C819.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. O. Hernandez-Gonzalez, C. L. Trevino, L. E. Castellano, J. L. de la Vega-Beltran, A. Y. Ocampo, E. Wertheimer, P. E. Visconti, and A. Darszon
Involvement of Cystic Fibrosis Transmembrane Conductance Regulator in Mouse Sperm Capacitation
J. Biol. Chem., August 17, 2007; 282(33): 24397 - 24406.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. L. Ong, X. Liu, K. Tsaneva-Atanasova, B. B. Singh, B. C. Bandyopadhyay, W. D. Swaim, J. T. Russell, R. S. Hegde, A. Sherman, and I. S. Ambudkar
Relocalization of STIM1 for Activation of Store-operated Ca2+ Entry Is Determined by the Depletion of Subplasma Membrane Endoplasmic Reticulum Ca2+ Store
J. Biol. Chem., April 20, 2007; 282(16): 12176 - 12185.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. E. Krouse, J. F. Talbott, M. M. Lee, N. S. Joo, and J. J. Wine
Acid and base secretion in the Calu-3 model of human serous cells
Am J Physiol Lung Cell Mol Physiol, December 1, 2004; 287(6): L1274 - L1283.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. M. Gonzalez-Guerrico, E. G. Cafferata, M. Radrizzani, F. Marcucci, D. Gruenert, O. H. Pivetta, R. R. Favaloro, R. Laguens, S. V. Perrone, G. C. Gallo, et al.
Tyrosine Kinase c-Src Constitutes a Bridge between Cystic Fibrosis Transmembrane Regulator Channel Failure and MUC1 Overexpression in Cystic Fibrosis
J. Biol. Chem., May 3, 2002; 277(19): 17239 - 17247.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
J. Choi, L. W. Hammer, and R. L. Hester
Calcium-Dependent Synthesis of Prostacyclin in ATP-Stimulated Venous Endothelial Cells
Hypertension, February 1, 2002; 39(2): 581 - 585.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
A. Bringmann, T. Pannicke, V. Moll, I. Milenkovic, F. Faude, V. Enzmann, S. Wolf, and A. Reichenbach
Upregulation of P2X7 Receptor Currents in Muller Glial Cells during Proliferative Vitreoretinopathy
Invest. Ophthalmol. Vis. Sci., March 1, 2001; 42(3): 860 - 867.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
T. P. Lockwich, X. Liu, B. B. Singh, J. Jadlowiec, S. Weiland, and I. S. Ambudkar
Assembly of Trp1 in a Signaling Complex Associated with Caveolin-Scaffolding Lipid Raft Domains
J. Biol. Chem., April 14, 2000; 275(16): 11934 - 11942.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.