| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 36, 25426-25432, September 3, 1999
From the Department of Pharmacology, The University of Iowa College
of Medicine, Iowa City, Iowa 52242
Previous results from this laboratory have shown
that human kidney (293) cells transfected with the rat follitropin
receptor (rFSHR) internalize agonist (i.e. human
follitropin, hFSH) at a rate similar to that of other agonist-G
protein-coupled receptor complexes while 293 cells transfected with the
rat lutropin/choriogonadotropin receptor (rLHR) internalize agonist
(human choriogonadotropin, hCG) at a rate that is about 1 order of
magnitude slower. Taking advantage of this difference and the high
degree of homology between the rLHR and rFSHR, we have now used
chimeras of these two receptors to begin to delineate structural
features that influence their internalization.
Analysis of six chimeras that exchanged only the transmembrane domains
(designated FLF and LFL), only the COOH-terminal domains (FFL or LLF)
or both domains (FLL or LFF) show that the origin of the extracellular
domain is at least as important, if not more, than the origin of the
transmembrane and COOH-terminal domains in determining the rate of
internalization of the gonadotropin receptors. Thus, the rates of
internalization of agonist internalization mediated by FFL, FLF, and
FLL more closely resemble rFSHR than rLHR, while the rates of agonist
internalization mediated by LLF, LFL, and LFF more closely resemble
rLHR than rFSHR.
The importance of the extracellular domain was also evident even upon
overexpression of arrestin-3, a protein that enhances the rate of
internalization of the wild-type receptors and chimeras by binding to
their intracellular regions.
This article has been cited by other articles:
|
|
 |
|
  C. Galet and M. Ascoli A Constitutively Active Mutant of the Human Lutropin Receptor (hLHR-L457R) Escapes Lysosomal Targeting and Degradation Mol. Endocrinol., November 1, 2006; 20(11): 2931 - 2945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yamashita, K. Nakamura, Y. Omori, K. Tsunekawa, M. Murakami, and T. Minegishi Association of Human Follitropin (FSH) Receptor with Splicing Variant of Human Lutropin/Choriogonadotropin Receptor Negatively Controls the Expression of Human FSH Receptor Mol. Endocrinol., August 1, 2005; 19(8): 2099 - 2111. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Galet, L. Min, R. Narayanan, M. Kishi, N. L. Weigel, and M. Ascoli Identification of a Transferable Two-Amino-Acid Motif (GT) Present in the C-Terminal Tail of the Human Lutropin Receptor that Redirects Internalized G Protein-Coupled Receptors from a Degradation to a Recycling Pathway Mol. Endocrinol., March 1, 2003; 17(3): 411 - 422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Sun and M. C. Gershengorn Correlation between Basal Signaling and Internalization of Thyrotropin-Releasing Hormone Receptors: Evidence for Involvement of Similar Receptor Conformations Endocrinology, August 1, 2002; 143(8): 2886 - 2892. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kishi, H. Krishnamurthy, C. Galet, R. S. Bhaskaran, and M. Ascoli Identification of a Short Linear Sequence Present in the C-terminal Tail of the Rat Follitropin Receptor That Modulates Arrestin-3 Binding in a Phosphorylation-independent Fashion J. Biol. Chem., June 7, 2002; 277(24): 21939 - 21946. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ascoli, F. Fanelli, and D. L. Segaloff The Lutropin/Choriogonadotropin Receptor, A 2002 Perspective Endocr. Rev., April 1, 2002; 23(2): 141 - 174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Min and M. Ascoli Effect of Activating and Inactivating Mutations on the Phosphorylation and Trafficking of the Human Lutropin/Choriogonadotropin Receptor Mol. Endocrinol., November 1, 2000; 14(11): 1797 - 1810. [Abstract] [Full Text]
|
|
|
|
|
|
M. Kishi and M. Ascoli The C-Terminal Tail of the Rat Lutropin/Choriogonadotropin (CG) Receptor Independently Modulates Human (h)CG-Induced Internalization of the Cell Surface Receptor and the Lysosomal Targeting of the Internalized hCG-Receptor Complex Mol. Endocrinol., June 1, 2000; 14(6): 926 - 936. [Abstract] [Full Text]
|
|
|
|
|
|
K. Nakamura, X. Liu, and M. Ascoli Seven Non-contiguous Intracellular Residues of the Lutropin/Choriogonadotropin Receptor Dictate the Rate of Agonist-induced Internalization and Its Sensitivity to Non-visual Arrestins J. Biol. Chem., January 7, 2000; 275(1): 241 - 247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kishi and M. Ascoli Multiple Distant Amino Acid Residues Present in the Serpentine Region of the Follitropin Receptor Modulate the Rate of Agonist-induced Internalization J. Biol. Chem., September 29, 2000; 275(40): 31030 - 31037. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Li, X. Liu, L. Min, and M. Ascoli Mutations of the Second Extracellular Loop of the Human Lutropin Receptor Emphasize the Importance of Receptor Activation and De-emphasize the Importance of Receptor Phosphorylation in Agonist-induced Internalization J. Biol. Chem., March 9, 2001; 276(11): 7968 - 7973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Min, C. Galet, and M. Ascoli The Association of Arrestin-3 with the Human Lutropin/Choriogonadotropin Receptor Depends Mostly on Receptor Activation Rather than on Receptor Phosphorylation J. Biol. Chem., January 4, 2002; 277(1): 702 - 710. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
