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J Biol Chem, Vol. 274, Issue 36, 25849-25854, September 3, 1999
From the Department of Biology, Georgia State University,
Atlanta, Georgia 30303
ArsA, an anion-stimulated ATPase, consists of two
nucleotide binding domains, A1 in the N terminus and A2 in the C
terminus of the protein, connected by a linker. The A1 domain contains a high affinity ATP binding site, whereas the A2 domain has low affinity and it requires the allosteric ligand antimonite for binding
ATP. ArsA is known to form a UV-activated adduct with [
-32P]ATP in the linker region. This study shows
that on addition of antimonite, much more adduct is formed.
Characterization of the nature of the adduct suggests that it is
between ArsA and ADP, instead of ATP, indicating that the adduct
formation reflects hydrolysis of ATP. The present study also
demonstrates that the A1 domain is capable of carrying out unisite
catalysis in the absence of antimonite. On addition of antimonite,
multisite catalysis involving both A1 and A2 sites occurs, resulting in
a 40-fold increase in ATPase activity. Studies with mutant proteins
suggest that the A2 site may be second in the sequence of events, so
that its role in catalysis is dependent on a functional A1 site. It is
also proposed that ArsA goes through an ATP-bound and an ADP-bound conformation, and the linker region, where ADP binds under both unisite
and multisite catalytic conditions, may play an important role in the
energy transduction process.
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