| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 37, 26165-26171, September 10, 1999
-Xylose
1,4-Galactosyltransferase I
4-GALACTOSYLTRANSFERASE GENE
FAMILY
§,,
,,, and
From the A seventh member of the human
School of Dentistry, University of
Copenhagen, Nørre Allé 20, 2200 Copenhagen N, Denmark, the
§ Institute of Molecular Pathology and Immunology of
University of Porto, Rua Dr. R. Frias s/n, 4200 Porto, Portugal, the
¶ University of Georgia, Complex Carbohydrate Research Center,
Athens, Georgia 30602, and the Institute of Physiological
Chemistry and Pathobiochemistry, University of Münster,
48129 Münster, Germany
4-galactosyltransferase family, 4Gal-T7, was identified by BLAST
analysis of expressed sequence tags. The coding region of 4Gal-T7
depicts a type II transmembrane protein with sequence similarity to
4-galactosyltransferases, but the sequence was distinct in known
motifs and did not contain the cysteine residues conserved in the other
six members of the 4Gal-T family. The genomic organization of
4Gal-T7 was different from previous 4Gal-Ts. Expression of
4Gal-T7 in insect cells showed that the gene product had
1,4-galactosyltransferase activity with -xylosides, and the
linkage formed was Gal1-4Xyl. Thus, 4Gal-T7 represents
galactosyltransferase I enzyme (xylosylprotein 1,4-galactosyltransferase; EC 2.4.1.133), which attaches the first
galactose in the proteoglycan linkage region
GlcA1-3Gal1-3Gal1-4Xyl1-O-Ser. Sequence analysis of
4Gal-T7 from a fibroblast cell line of a patient with a progeroid
syndrome and signs of the Ehlers-Danlos syndrome, previously shown to
exhibit reduced galactosyltransferase I activity (Quentin, E., Gladen,
A., Rodén, L., and Kresse, H. (1990) Proc. Natl. Acad. Sci.
U. S. A. 87, 1342-1346), revealed two inherited allelic
variants, 4Gal-T7186D and 4Gal-T7206P,
each with a single missense substitution in the putative catalytic domain of the enzyme. 4Gal-T7186D exhibited a 4-fold
elevated Km for the donor substrate, whereas
essentially no activity was demonstrated with
4Gal-T7206P. Molecular cloning of 4Gal-T7 should
facilitate general studies of its pathogenic role in progeroid
syndromes and connective tissue disorders with affected proteoglycan biosynthesis.
This article has been cited by other articles:
|
|
 |
|
  M. Gotte, D. Spillmann, G. W. Yip, E. Versteeg, F. G. Echtermeyer, T. H. van Kuppevelt, and L. Kiesel Changes in heparan sulfate are associated with delayed wound repair, altered cell migration, adhesion and contractility in the galactosyltransferase I (ss4GalT-7) deficient form of Ehlers-Danlos syndrome Hum. Mol. Genet., April 1, 2008; 17(7): 996 - 1009. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ueyama, H. Takemae, Y. Ohmae, H. Yoshida, H. Toyoda, R. Ueda, and S. Nishihara Functional Analysis of Proteoglycan Galactosyltransferase II RNA Interference Mutant Flies J. Biol. Chem., March 7, 2008; 283(10): 6076 - 6084. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gulberti, V. Lattard, M. Fondeur, J.-C. Jacquinet, G. Mulliert, P. Netter, J. Magdalou, M. Ouzzine, and S. Fournel-Gigleux Phosphorylation and Sulfation of Oligosaccharide Substrates Critically Influence the Activity of Human {beta}1,4-Galactosyltransferase 7 (GalT-I) and {beta}1,3-Glucuronosyltransferase I (GlcAT-I) Involved in the Biosynthesis of the Glycosaminoglycan-Protein Linkage Region of Proteoglycans J. Biol. Chem., January 14, 2005; 280(2): 1417 - 1425. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Young, T. Stauber, E. del Nery, I. Vernos, R. Pepperkok, and T. Nilsson Regulation of Microtubule-dependent Recycling at the Trans-Golgi Network by Rab6A and Rab6A' Mol. Biol. Cell, January 1, 2005; 16(1): 162 - 177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. T. Marcos, S. Pinho, C. Grandela, A. Cruz, B. Samyn-Petit, A. Harduin-Lepers, R. Almeida, F. Silva, V. Morais, J. Costa, et al. Role of the Human ST6GalNAc-I and ST6GalNAc-II in the Synthesis of the Cancer-Associated Sialyl-Tn Antigen Cancer Res., October 1, 2004; 64(19): 7050 - 7057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Vadaie and D. L. Jarvis Molecular Cloning and Functional Characterization of a Lepidopteran Insect {beta}4-N-Acetylgalactosaminyltransferase with Broad Substrate Specificity, a Functional Role in Glycoprotein Biosynthesis, and a Potential Functional Role in Glycolipid Biosynthesis J. Biol. Chem., August 6, 2004; 279(32): 33501 - 33518. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Rodeheffer and B. D. Shur Characterization of a novel ZP3-independent sperm-binding ligand that facilitates sperm adhesion to the egg coat Development, February 1, 2004; 131(3): 503 - 512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sato, M. Gotoh, K. Kiyohara, A. Kameyama, T. Kubota, N. Kikuchi, Y. Ishizuka, H. Iwasaki, A. Togayachi, T. Kudo, et al. Molecular Cloning and Characterization of a Novel Human {beta}1,4-N-Acetylgalactosaminyltransferase, {beta}4GalNAc-T3, Responsible for the Synthesis of N,N'-Diacetyllactosediamine, GalNAc{beta}1-4GlcNAc J. Biol. Chem., November 28, 2003; 278(48): 47534 - 47544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yada, T. Sato, H. Kaseyama, M. Gotoh, H. Iwasaki, N. Kikuchi, Y.-D. Kwon, A. Togayachi, T. Kudo, H. Watanabe, et al. Chondroitin Sulfate Synthase-3: MOLECULAR CLONING AND CHARACTERIZATION J. Biol. Chem., October 10, 2003; 278(41): 39711 - 39725. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P J Coucke, M W Wessels, P Van Acker, R Gardella, S Barlati, P J Willems, M Colombi, and A De Paepe Homozygosity mapping of a gene for arterial tortuosity syndrome to chromosome 20q13 J. Med. Genet., October 1, 2003; 40(10): 747 - 751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yada, M. Gotoh, T. Sato, M. Shionyu, M. Go, H. Kaseyama, H. Iwasaki, N. Kikuchi, Y.-D. Kwon, A. Togayachi, et al. Chondroitin Sulfate Synthase-2: MOLECULAR CLONING AND CHARACTERIZATION OF A NOVEL HUMAN GLYCOSYLTRANSFERASE HOMOLOGOUS TO CHONDROITIN SULFATE GLUCURONYLTRANSFERASE, WHICH HAS DUAL ENZYMATIC ACTIVITIES J. Biol. Chem., August 8, 2003; 278(32): 30235 - 30247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jonsson, E. Eklund, L.-A. Fransson, and A. Oldberg Initiation of the Decorin Glycosaminoglycan Chain in the Endoplasmic Reticulum-Golgi Intermediate Compartment J. Biol. Chem., June 6, 2003; 278(24): 21415 - 21420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Takemae, R. Ueda, R. Okubo, H. Nakato, S. Izumi, K. Saigo, and S. Nishihara Proteoglycan UDP-Galactose:beta -Xylose beta 1,4-Galactosyltransferase I Is Essential for Viability in Drosophila melanogaster J. Biol. Chem., April 25, 2003; 278(18): 15571 - 15578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-Y. Hwang, S. K. Olson, J. R. Brown, J. D. Esko, and H. R. Horvitz The Caenorhabditis elegans Genes sqv-2 and sqv-6, Which Are Required for Vulval Morphogenesis, Encode Glycosaminoglycan Galactosyltransferase II and Xylosyltransferase J. Biol. Chem., March 28, 2003; 278(14): 11735 - 11738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-T. Kim, K. Tsuchida, J. Lincecum, H. Kitagawa, M. Bernfield, and K. Sugahara Identification and Characterization of Three Drosophila melanogaster Glucuronyltransferases Responsible for the Synthesis of the Conserved Glycosaminoglycan-Protein Linkage Region of Proteoglycans. TWO NOVEL HOMOLOGS EXHIBIT BROAD SPECIFICITY TOWARD OLIGOSACCHARIDES FROM PROTEOGLYCANS, GLYCOPROTEINS, AND GLYCOSPHINGOLIPIDS J. Biol. Chem., March 7, 2003; 278(11): 9116 - 9124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Seko, N. Dohmae, K. Takio, and K. Yamashita beta 1,4-Galactosyltransferase (beta 4GalT)-IV Is Specific for GlcNAc 6-O-Sulfate. beta 4GalT-IV ACTS ON KERATAN SULFATE-RELATED GLYCANS AND A PRECURSOR GLYCAN OF 6-SULFOSIALYL-LEWIS X J. Biol. Chem., March 7, 2003; 278(11): 9150 - 9158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sato, M. Gotoh, K. Kiyohara, T. Akashima, H. Iwasaki, A. Kameyama, H. Mochizuki, T. Yada, N. Inaba, A. Togayachi, et al. Differential Roles of Two N-Acetylgalactosaminyltransferases, CSGalNAcT-1, and a Novel Enzyme, CSGalNAcT-2. INITIATION AND ELONGATION IN SYNTHESIS OF CHONDROITIN SULFATE J. Biol. Chem., January 24, 2003; 278(5): 3063 - 3071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Nakamura, N. Haines, J. Chen, T. Okajima, K. Furukawa, T. Urano, P. Stanley, K. D. Irvine, and K. Furukawa Identification of a Drosophila Gene Encoding Xylosylprotein beta 4-Galactosyltransferase That Is Essential for the Synthesis of Glycosaminoglycans and for Morphogenesis J. Biol. Chem., November 22, 2002; 277(48): 46280 - 46288. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-Y. Hwang and H. R. Horvitz The SQV-1 UDP-glucuronic acid decarboxylase and the SQV-7 nucleotide-sugar transporter may act in the Golgi apparatus to affect Caenorhabditis elegans vulval morphogenesis and embryonic development PNAS, October 29, 2002; 99(22): 14218 - 14223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-Y. Hwang and H. R. Horvitz The Caenorhabditis elegans vulval morphogenesis gene sqv-4 encodes a UDP-glucose dehydrogenase that is temporally and spatially regulated PNAS, October 29, 2002; 99(22): 14224 - 14229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zheng, I. Van Die, and R. D. Cummings Molecular Cloning and Characterization of a Novel alpha 1,2-Fucosyltransferase (CE2FT-1) from Caenorhabditis elegans J. Biol. Chem., October 11, 2002; 277(42): 39823 - 39832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gotoh, T. Yada, T. Sato, T. Akashima, H. Iwasaki, H. Mochizuki, N. Inaba, A. Togayachi, T. Kudo, H. Watanabe, et al. Molecular Cloning and Characterization of a Novel Chondroitin Sulfate Glucuronyltransferase That Transfers Glucuronic Acid to N-Acetylgalactosamine J. Biol. Chem., October 4, 2002; 277(41): 38179 - 38188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gotoh, T. Sato, T. Akashima, H. Iwasaki, A. Kameyama, H. Mochizuki, T. Yada, N. Inaba, Y. Zhang, N. Kikuchi, et al. Enzymatic Synthesis of Chondroitin with a Novel Chondroitin Sulfate N-Acetylgalactosaminyltransferase That Transfers N-Acetylgalactosamine to Glucuronic Acid in Initiation and Elongation of Chondroitin Sulfate Synthesis J. Biol. Chem., October 4, 2002; 277(41): 38189 - 38196. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Vadaie, R. S. Hulinsky, and D. L. Jarvis Identification and characterization of a Drosophila melanogaster ortholog of human {beta}1,4-galactosyltransferase VII Glycobiology, October 1, 2002; 12(10): 589 - 597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. S. Kawar, I. Van Die, and R. D. Cummings Molecular Cloning and Enzymatic Characterization of a UDP-GalNAc:GlcNAcbeta -R beta 1,4-N-Acetylgalactosaminyltransferase from Caenorhabditis elegans J. Biol. Chem., September 13, 2002; 277(38): 34924 - 34932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yamada, Y. Okada, M. Ueno, S. Iwata, S. S. Deepa, S. Nishimura, M. Fujita, I. Van Die, Y. Hirabayashi, and K. Sugahara Determination of the Glycosaminoglycan-Protein Linkage Region Oligosaccharide Structures of Proteoglycans from Drosophila melanogaster and Caenorhabditis elegans J. Biol. Chem., August 23, 2002; 277(35): 31877 - 31886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Boeggeman and P. K. Qasba Studies on the metal binding sites in the catalytic domain of {beta}1,4-galactosyltransferase Glycobiology, July 1, 2002; 12(7): 395 - 407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. B. H. Wilson Functional Characterization of Drosophila melanogaster Peptide O-Xylosyltransferase, the Key Enzyme for Proteoglycan Chain Initiation and Member of the Core 2/I N-Acetylglucosaminyltransferase Family J. Biol. Chem., June 7, 2002; 277(24): 21207 - 21212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Bai, D. Zhou, J. R. Brown, B. E. Crawford, T. Hennet, and J. D. Esko Biosynthesis of the Linkage Region of Glycosaminoglycans. CLONING AND ACTIVITY OF GALACTOSYLTRANSFERASE II, THE SIXTH MEMBER OF THE beta 1,3-GALACTOSYLTRANSFERASE FAMILY (beta 3GalT6) J. Biol. Chem., December 14, 2001; 276(51): 48189 - 48195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. H. Freeze Update and perspectives on congenital disorders of glycosylation Glycobiology, December 1, 2001; 11(12): 129R - 143R. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Chen, D. J. Moloney, and P. Stanley Fringe modulation of Jagged1-induced Notch signaling requires the action of beta 4galactosyltransferase-1 PNAS, November 9, 2001; (2001) 241398098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Guo, T. Sato, K. Shirane, and K. Furukawa Galactosylation of N-linked oligosaccharides by human {beta}-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells Glycobiology, October 1, 2001; 11(10): 813 - 820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.-G. Hanisch, C. A. Reis, H. Clausen, and H. Paulsen Evidence for glycosylation-dependent activities of polypeptide N-acetylgalactosaminyltransferases rGalNAc-T2 and -T4 on mucin glycopeptides Glycobiology, September 1, 2001; 11(9): 731 - 740. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Berninsone, H.-Y. Hwang, I. Zemtseva, H. R. Horvitz, and C. B. Hirschberg SQV-7, a protein involved in Caenorhabditis elegans epithelial invagination and early embryogenesis, transports UDP-glucuronic acid, UDP-N- acetylgalactosamine, and UDP-galactose PNAS, March 16, 2001; (2001) 61593098. [Abstract] [Full Text]
|
|
|
|
|
|
D. A. Bulik, G. Wei, H. Toyoda, A. Kinoshita-Toyoda, W. R. Waldrip, J. D. Esko, P. W. Robbins, and S. B. Selleck sqv-3, -7, and -8, a set of genes affecting morphogenesis in Caenorhabditis elegans, encode enzymes required for glycosaminoglycan biosynthesis PNAS, September 26, 2000; 97(20): 10838 - 10843. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Bouhours, J. Liaigre, J. Naulet, N. V.Bovin, and J.-F. Bouhours A novel pentaglycosylceramide in ostrich liver, IV4-{beta}-Gal-nLc4Cer, with terminal Gal({beta}1-4)Gal, a xenoepitope recognized by human natural antibodies Glycobiology, September 1, 2000; 10(9): 857 - 864. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K Prydz and K. Dalen Synthesis and sorting of proteoglycans J. Cell Sci., January 1, 2000; 113(2): 193 - 205. [Abstract] [PDF]
|
|
|
|
|
|
H. Kitagawa, N. Egusa, J.-i. Tamura, M. Kusche-Gullberg, U. Lindahl, and K. Sugahara rib-2, a Caenorhabditis elegans Homolog of the Human Tumor Suppressor EXT Genes Encodes a Novel alpha 1,4-N-Acetylglucosaminyltransferase Involved in the Biosynthetic Initiation and Elongation of Heparan Sulfate J. Biol. Chem., February 9, 2001; 276(7): 4834 - 4838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Shiraishi, A. Natsume, A. Togayachi, T. Endo, T. Akashima, Y. Yamada, N. Imai, S. Nakagawa, S. Koizumi, S. Sekine, et al. Identification and Characterization of Three Novel beta 1,3-N-Acetylglucosaminyltransferases Structurally Related to the beta 1,3-Galactosyltransferase Family J. Biol. Chem., January 26, 2001; 276(5): 3498 - 3507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Ramakrishnan, P. S. Shah, and P. K. Qasba alpha -Lactalbumin (LA) Stimulates Milk beta -1,4-Galactosyltransferase I (beta 4Gal-T1) to Transfer Glucose from UDP-glucose to N-Acetylglucosamine. CRYSTAL STRUCTURE OF beta 4Gal-T1{middle dot}LA COMPLEX WITH UDP-Glc J. Biol. Chem., September 28, 2001; 276(40): 37665 - 37671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Berninsone, H.-Y. Hwang, I. Zemtseva, H. R. Horvitz, and C. B. Hirschberg SQV-7, a protein involved in Caenorhabditis elegans epithelial invagination and early embryogenesis, transports UDP-glucuronic acid, UDP-N- acetylgalactosamine, and UDP-galactose PNAS, March 27, 2001; 98(7): 3738 - 3743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Chen, D. J. Moloney, and P. Stanley Fringe modulation of Jagged1-induced Notch signaling requires the action of beta 4galactosyltransferase-1 PNAS, November 20, 2001; 98(24): 13716 - 13721. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
