HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brown, E. L. Articles by Höök, M. Search for Related Content PubMed PubMed Citation Articles by Brown, E. L. Articles by Höök, M. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 37, 26272-26278, September 10, 1999

Adherence of Borrelia burgdorferi
IDENTIFICATION OF CRITICAL LYSINE RESIDUES IN DbpA REQUIRED FOR DECORIN BINDING

From the Center for Extracellular Matrix Biology, Albert B. Alkek Institute of Biosciences and Technology, Texas A&M University Health Science Center, Houston, Texas 77030

Borrelia burgdorferi, the causative agent of Lyme disease, expresses on its surface two decorin binding adhesins, DbpA and DbpB. Previous studies have demonstrated that vaccination of mice with DbpA provided protection against challenge with heterologous Borrelia strains despite considerable sequence variability among DbpA in these strains.

We have now examined the importance of individual amino acid residues in DbpA for decorin binding. We demonstrated that chemical modification of lysine residues resulted in loss of ligand binding activity. Of the 27 lysine residues in native DbpA from strain 297, 6 are present in most and 5 are conserved in all 30 DbpA sequences examined so far. Analysis of recombinant DbpA in which individual lysine residues have been mutated to alanine suggested that three of the conserved residues distributed throughout the DbpA sequence are required for decorin binding. These mutants lost their ability to bind decorin in Western ligand blot assay and bound reduced amounts of decorin in an ELISA. Furthermore, these mutant DbpA proteins did not inhibit the adherence of B. burgdorferi to a decorin substrata, and they did not recognize decorin in an extracellular matrix established by human fibroblast cultures. We conclude that the three lysine residues Lys-82, Lys-163, and Lys-170 are crucial for the binding of DbpA to decorin.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				Y. Shi, Q. Xu, K. McShan, and F. T. Liang Both Decorin-Binding Proteins A and B Are Critical for the Overall Virulence of Borrelia burgdorferi Infect. Immun., March 1, 2008; 76(3): 1239 - 1246. [Abstract] [Full Text] [PDF]

				Q. Xu, S. V. Seemanaplli, K. McShan, and F. T. Liang Increasing the Interaction of Borrelia burgdorferi with Decorin Significantly Reduces the 50 Percent Infectious Dose and Severely Impairs Dissemination Infect. Immun., September 1, 2007; 75(9): 4272 - 4281. [Abstract] [Full Text] [PDF]

				Y. Shi, Q. Xu, S. V. Seemanapalli, K. McShan, and F. T. Liang The dbpBA Locus of Borrelia burgdorferi Is Not Essential for Infection of Mice Infect. Immun., November 1, 2006; 74(11): 6509 - 6512. [Abstract] [Full Text] [PDF]

				A. S. Barbosa, P. A. E. Abreu, F. O. Neves, M. V. Atzingen, M. M. Watanabe, M. L. Vieira, Z. M. Morais, S. A. Vasconcellos, and A. L. T. O. Nascimento A Newly Identified Leptospiral Adhesin Mediates Attachment to Laminin Infect. Immun., November 1, 2006; 74(11): 6356 - 6364. [Abstract] [Full Text] [PDF]

				F. T. Liang, J. Yan, M. L. Mbow, S. L. Sviat, R. D. Gilmore, M. Mamula, and E. Fikrig Borrelia burgdorferi Changes Its Surface Antigenic Expression in Response to Host Immune Responses Infect. Immun., October 1, 2004; 72(10): 5759 - 5767. [Abstract] [Full Text] [PDF]

				J. H. Kim, J. Singvall, U. Schwarz-Linek, B. J. B. Johnson, J. R. Potts, and M. Hook BBK32, a Fibronectin Binding MSCRAMM from Borrelia burgdorferi, Contains a Disordered Region That Undergoes a Conformational Change on Ligand Binding J. Biol. Chem., October 1, 2004; 279(40): 41706 - 41714. [Abstract] [Full Text] [PDF]

				F. T. Liang, E. L. Brown, T. Wang, R. V. Iozzo, and E. Fikrig Protective Niche for Borrelia burgdorferi to Evade Humoral Immunity Am. J. Pathol., September 1, 2004; 165(3): 977 - 985. [Abstract] [Full Text] [PDF]

				M. C. Zambrano, A. A. Beklemisheva, A. V. Bryksin, S. A. Newman, and F. C. Cabello Borrelia burgdorferi Binds to, Invades, and Colonizes Native Type I Collagen Lattices Infect. Immun., June 1, 2004; 72(6): 3138 - 3146. [Abstract] [Full Text] [PDF]

				X. F. Yang, S. M. Alani, and M. V. Norgard The response regulator Rrp2 is essential for the expression of major membrane lipoproteins in Borrelia burgdorferi PNAS, September 16, 2003; 100(19): 11001 - 11006. [Abstract] [Full Text] [PDF]

				D. S. Pikas, E. L. Brown, S. Gurusiddappa, L. Y. Lee, Y. Xu, and M. Hook Decorin-binding Sites in the Adhesin DbpA from Borrelia burgdorferi: A SYNTHETIC PEPTIDE APPROACH J. Biol. Chem., August 15, 2003; 278(33): 30920 - 30926. [Abstract] [Full Text] [PDF]

				J. R. Fischer, N. Parveen, L. Magoun, and J. M. Leong Decorin-binding proteins A and B confer distinct mammalian cell type-specific attachment by Borrelia burgdorferi, the Lyme disease spirochete PNAS, June 10, 2003; 100(12): 7307 - 7312. [Abstract] [Full Text] [PDF]

				T. Heikkila, I. Seppala, H. Saxen, J. Panelius, H. Yrjanainen, and P. Lahdenne Species-Specific Serodiagnosis of Lyme Arthritis and Neuroborreliosis Due to Borrelia burgdorferi Sensu Stricto, B. afzelii, and B. garinii by Using Decorin Binding Protein A J. Clin. Microbiol., February 1, 2002; 40(2): 453 - 460. [Abstract] [Full Text] [PDF]

				N. D. Ulbrandt, D. R. Cassatt, N. K. Patel, W. C. Roberts, C. M. Bachy, C. A. Fazenbaker, and M. S. Hanson Conformational Nature of the Borrelia burgdorferi Decorin Binding Protein A Epitopes That Elicit Protective Antibodies Infect. Immun., August 1, 2001; 69(8): 4799 - 4807. [Abstract] [Full Text] [PDF]

				K. E. Hagman, X. Yang, S. K. Wikel, G. B. Schoeler, M. J. Caimano, J. D. Radolf, and M. V. Norgard Decorin-Binding Protein A (DbpA) of Borrelia burgdorferi Is Not Protective When Immunized Mice Are Challenged via Tick Infestation and Correlates with the Lack of DbpA Expression by B. burgdorferi in Ticks Infect. Immun., August 1, 2000; 68(8): 4759 - 4764. [Abstract] [Full Text] [PDF]

				D. A. Haake Spirochaetal lipoproteins and pathogenesis Microbiology, July 1, 2000; 146(7): 1491 - 1504. [Full Text]