|
J Biol Chem, Vol. 274, Issue 37, 26337-26343, September 10, 1999
Nitric Oxide Modulates 2-Adrenergic Receptor
Palmitoylation and Signaling
Lynda
Adam,
Michel
Bouvier, and
Teresa L. Z.
Jones¶
From the Département de Biochimie and Le Groupe de Recherche
sur le Système Nerveux Autonome, Université de
Montréal, Montréal, Quebec H3T 1J4, Canada and
¶ Metabolic Diseases Branch, NIDDK, National Institutes of
Health, Bethesda, Maryland 20892
To determine whether nitric oxide (NO) modulates
the -adrenergic signaling pathway, we treated cells expressing
2-adrenergic receptors ( 2AR) with
the NO donors, 3-morpholinosydnonimine (SIN-1) and
1,2,3,4-oxatriazolium,5-amino-3-(3-chloro-2-methylphenyl)chloride and determined the intracellular production of cAMP after
exposure to -adrenergic receptor agonists, cholera toxin and
forskolin. NO significantly decreased the potency of the -adrenergic
agonist, isoproterenol, to stimulate cAMP production without affecting the stimulatory action of forskolin and cholera toxin, which directly activate adenylyl cyclase and Gs, respectively. Treatment
with the NO donor increased the guanyl nucleotide-sensitive high
affinity constant for the agonist, isoproterenol, thus suggesting that it reduced functional coupling between the receptor and Gs.
Stimulation of endogenous NO production by lipopolysaccharide in RAW
264.7 macrophages also caused a significant increase in the
EC50 for isoproterenol-stimulated cAMP production. SIN-1
treatment also led to a reduction in both basal and
isoproterenol-stimulated incorporation of [3H]palmitate
into the 2AR. Signaling through the nonpalmitoylated, Gly341 2AR mutant was unchanged by SIN-1
treatment. Given the link between 2AR palmitoylation and
its responsiveness to agonist, these results suggest that the primary
action of NO was depalmitoylation of the 2AR resulting
in decreased signaling through the 2AR.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.

CiteULike Complore Connotea Del.icio.us Digg Reddit Technorati What's this?
This article has been cited by other articles:

|
 |

|
 |
 
M. G. Marko, H.-J. E. Pang, Z. Ren, A. Azzi, B. T. Huber, S. C. Bunnell, and S. N. Meydani
Vitamin E Reverses Impaired Linker for Activation of T Cells Activation in T Cells from Aged C57BL/6 Mice
J. Nutr.,
June 1, 2009;
139(6):
1192 - 1197.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
B. Chini and M. Parenti
G-protein-coupled receptors, cholesterol and palmitoylation: facts about fats
J. Mol. Endocrinol.,
May 1, 2009;
42(5):
371 - 379.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. Rodriguez-Melendez and J. Zempleni
Nitric Oxide Signaling Depends on Biotin in Jurkat Human Lymphoma Cells
J. Nutr.,
March 1, 2009;
139(3):
429 - 433.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. S. Lee, S. R. Kim, S. J. Park, H. S. Park, K. H. Min, M. H. Lee, S. M. Jin, G. Y. Jin, W. H. Yoo, and Y. C. Lee
Hydrogen Peroxide Induces Vascular Permeability via Regulation of Vascular Endothelial Growth Factor
Am. J. Respir. Cell Mol. Biol.,
August 1, 2006;
35(2):
190 - 197.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
P. Linscheid, D. Seboek, H. Zulewski, A. Scherberich, N. Blau, U. Keller, and B. Muller
Cytokine-induced metabolic effects in human adipocytes are independent of endogenous nitric oxide
Am J Physiol Endocrinol Metab,
June 1, 2006;
290(6):
E1068 - E1077.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
E. Nozik-Grayck, E. J. Whalen, J. S. Stamler, T. J. McMahon, P. Chitano, and C. A. Piantadosi
S-nitrosoglutathione inhibits {alpha}1-adrenergic receptor-mediated vasoconstriction and ligand binding in pulmonary artery
Am J Physiol Lung Cell Mol Physiol,
January 1, 2006;
290(1):
L136 - L143.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
T. Rangasamy, J. Guo, W. A. Mitzner, J. Roman, A. Singh, A. D. Fryer, M. Yamamoto, T. W. Kensler, R. M. Tuder, S. N. Georas, et al.
Disruption of Nrf2 enhances susceptibility to severe airway inflammation and asthma in mice
J. Exp. Med.,
July 5, 2005;
202(1):
47 - 59.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Azzi, P. G. Charest, S. Angers, G. Rousseau, T. Kohout, M. Bouvier, and G. Pineyro
{beta}-Arrestin-mediated activation of MAPK by inverse agonists reveals distinct active conformations for G protein-coupled receptors
PNAS,
September 30, 2003;
100(20):
11406 - 11411.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
D. Merkus, B. Houweling, A. Mirza, F. Boomsma, A. H van den Meiracker, and D. J Duncker
Contribution of endothelin and its receptors to the regulation of vascular tone during exercise is different in the systemic, coronary and pulmonary circulation
Cardiovasc Res,
September 1, 2003;
59(3):
745 - 754.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
E. N Dedkova, Y. Gao Wang, L. A Blatter, and S. L Lipsius
Nitric oxide signalling by selective {beta}2-adrenoceptor stimulation prevents ACh-induced inhibition of {beta}2-stimulated Ca2+ current in cat atrial myocytes
J. Physiol.,
August 1, 2002;
542(3):
711 - 723.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
N. Abi-Gerges, G. Szabo, A. S Otero, R. Fischmeister, and P.-F. Mery
NO donors potentiate the {beta}-adrenergic stimulation of ICa,L and the muscarinic activation of IK,ACh in rat cardiac myocytes
J. Physiol.,
April 15, 2002;
540(2):
411 - 424.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. A. Santizo, H. M. Koenig, and D. A. Pelligrino
{beta}-Adrenoceptor and nNOS-derived NO interactions modulate hypoglycemic pial arteriolar dilation in rats
Am J Physiol Heart Circ Physiol,
February 1, 2001;
280(2):
H562 - H568.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Restituito, T. Cens, C. Barrere, S. Geib, S. Galas, M. De Waard, and P. Charnet
The {beta}2a Subunit Is a Molecular Groom for the Ca2+ Channel Inactivation Gate
J. Neurosci.,
December 15, 2000;
20(24):
9046 - 9052.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
T. L. Baker, M. A. Booden, and J. E. Buss
S-Nitrosocysteine Increases Palmitate Turnover on Ha-Ras in NIH 3T3 Cells
J. Biol. Chem.,
July 14, 2000;
275(29):
22037 - 22047.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
E. Nozik-Grayck, T. J. McMahon, Y.-C. T. Huang, C. S. Dieterle, J. S. Stamler, and C. A. Piantadosi
Pulmonary vasoconstriction by serotonin is inhibited by S-nitrosoglutathione
Am J Physiol Lung Cell Mol Physiol,
May 1, 2002;
282(5):
L1057 - L1065.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
N. Abi-Gerges, G. Szabo, A. S. Otero, R. Fischmeister, and P.-F. Mery
NO donors potentiate the {beta}-adrenergic stimulation of ICa,L and the muscarinic activation of IK in rat cardiac myocytes
J. Physiol.,
February 22, 2002;
(2002)
200101292.
[Abstract]
[PDF]
|
 |
|
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.
|
Advertisement
Advertisement
|