| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 38, 26815-26821, September 17, 1999
12-induced Cytoskeletal Responses
,,,
From the Thrombin induces astrocytoma cell rounding
through a Rho-dependent pathway (Majumdar, M., Seasholtz,
T. M., Goldstein, D., de Lanerolle, P., and Brown, J. H. (1998) J. Biol. Chem. 273, 10099-10106). The
involvement of the G12 family of G proteins and the role of
specific Rho exchange factors in transducing signals from the thrombin
receptor to Rho-dependent cytoskeletal responses was
examined. Microinjection of cDNAs for activated G Department of Pharmacology, University of
California, San Diego, La Jolla, California 92093-0636 and the
§ Department of Physiology, Tufts University,
Boston, Massachusetts 02111
12
or G13 induced cell rounding, and antibodies to
G12 or G13 blocked the response to
thrombin. In contrast, activation or inhibition of Gq
function had relatively little effect. The cytoskeletal response to
G12 was inhibited by microinjection of C3 exoenzyme, indicating Rho dependence. Two Rho-specific guanine nucleotide exchange
factors (GEFs), oncogenic lbc and p115, increased the percentage of
rounded cells 4-5-fold, and this was inhibited by C3. Mutant GEFs
lacking the Dbl homology (DH) domain required for exchange factor
activity failed to induce cell rounding. However, the DH mutants of lbc
and p115 were efficacious inhibitors of rounding induced by thrombin or
G12. The effects of lbc were dependent on an intact
pleckstrin homology domain, which may be required for appropriate
targeting of the Rho-GEF. These findings identify the
G12 protein family as transducers of thrombin signaling to the cytoskeleton and provide the first evidence that a Rho-GEF transduces signals between G protein-coupled receptors and Rho-mediated cytoskeletal responses.
This article has been cited by other articles:
|
|
 |
|
  M. P. Iwanicki, T. Vomastek, R. W. Tilghman, K. H. Martin, J. Banerjee, P. B. Wedegaertner, and J. T. Parsons FAK, PDZ-RhoGEF and ROCKII cooperate to regulate adhesion movement and trailing-edge retraction in fibroblasts J. Cell Sci., March 15, 2008; 121(6): 895 - 905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Shibolet, C. Giallourakis, I. Rosenberg, T. Mueller, R. J. Xavier, and D. K. Podolsky AKAP13, a RhoA GTPase-specific Guanine Exchange Factor, Is a Novel Regulator of TLR2 Signaling J. Biol. Chem., November 30, 2007; 282(48): 35308 - 35317. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Carlile-Klusacek and V. Rizzo Endothelial cytoskeletal reorganization in response to PAR1 stimulation is mediated by membrane rafts but not caveolae Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H366 - H375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Sandquist, K. I. Swenson, K. A. DeMali, K. Burridge, and A. R. Means Rho Kinase Differentially Regulates Phosphorylation of Nonmuscle Myosin II Isoforms A and B during Cell Rounding and Migration J. Biol. Chem., November 24, 2006; 281(47): 35873 - 35883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. K. Peterson, P. Kelly, C. A. Weinbaum, and P. J. Casey A Novel Protein Geranylgeranyltransferase-I Inhibitor with High Potency, Selectivity, and Cellular Activity J. Biol. Chem., May 5, 2006; 281(18): 12445 - 12450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Wettschureck and S. Offermanns Mammalian G Proteins and Their Cell Type Specific Functions Physiol Rev, October 1, 2005; 85(4): 1159 - 1204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. N. McLaughlin, L. Shen, M. Holinstat, J. D. Brooks, E. DiBenedetto, and H. E. Hamm Functional Selectivity of G Protein Signaling by Agonist Peptides and Thrombin for the Protease-activated Receptor-1 J. Biol. Chem., July 1, 2005; 280(26): 25048 - 25059. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Meigs, J. Juneja, C. T. DeMarco, L. N. Stemmle, D. D. Kaplan, and P. J. Casey Selective Uncoupling of G{alpha}12 from Rho-mediated Signaling J. Biol. Chem., May 6, 2005; 280(18): 18049 - 18055. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ohkawara, T. Ishibashi, T. Sakamoto, K. Sugimoto, K. Nagata, K. Yokoyama, N. Sakamoto, M. Kamioka, I. Matsuoka, S. Fukuhara, et al. Thrombin-induced Rapid Geranylgeranylation of RhoA as an Essential Process for RhoA Activation in Endothelial Cells J. Biol. Chem., March 18, 2005; 280(11): 10182 - 10188. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Steinhoff, J. Buddenkotte, V. Shpacovitch, A. Rattenholl, C. Moormann, N. Vergnolle, T. A. Luger, and M. D. Hollenberg Proteinase-Activated Receptors: Transducers of Proteinase-Mediated Signaling in Inflammation and Immune Response Endocr. Rev., February 1, 2005; 26(1): 1 - 43. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. McNeil Coffield, W. S. Helms, Q. Jiang, and L. Su G{alpha}13 Mediates a Signal That Is Essential for Proliferation and Survival of Thymocyte Progenitors J. Exp. Med., November 15, 2004; 200(10): 1315 - 1324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Wang, M. Liu, T. Kozasa, J. D. Rothstein, P. C. Sternweis, and R. R. Neubig Thrombin and Lysophosphatidic Acid Receptors Utilize Distinct rhoGEFs in Prostate Cancer Cells J. Biol. Chem., July 9, 2004; 279(28): 28831 - 28834. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zhuang, G. T. Nguyen, Y. Chen, T. Gudi, M. Eigenthaler, T. Jarchau, U. Walter, G. R. Boss, and R. B. Pilz Vasodilator-stimulated Phosphoprotein Activation of Serum-response Element-dependent Transcription Occurs Downstream of RhoA and Is Inhibited by cGMP-dependent Protein Kinase Phosphorylation J. Biol. Chem., March 12, 2004; 279(11): 10397 - 10407. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. McGrew, R. D. Price, E. Hackler, M. S. S. Chang, and E. Sanders-Bush RNA Editing of the Human Serotonin 5-HT2C Receptor Disrupts Transactivation of the Small G-Protein RhoA Mol. Pharmacol., January 1, 2004; 65(1): 252 - 256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. N. Meyer, J. Hunt, C. Schwesinger, and B. M. Denker G{alpha}12 regulates epithelial cell junctions through Src tyrosine kinases Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1281 - C1293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. SOMLYO and A. V. SOMLYO Ca2+ Sensitivity of Smooth Muscle and Nonmuscle Myosin II: Modulated by G Proteins, Kinases, and Myosin Phosphatase Physiol Rev, October 1, 2003; 83(4): 1325 - 1358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Miyamoto, J. Yamauchi, and H. Itoh Src Kinase Regulates the Activation of a Novel FGD-1-related Cdc42 Guanine Nucleotide Exchange Factor in the Signaling Pathway from the Endothelin A Receptor to JNK J. Biol. Chem., August 8, 2003; 278(32): 29890 - 29900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yuan, L. W. Slice, J. Gu, and E. Rozengurt Cooperation of Gq, Gi, and G12/13 in Protein Kinase D Activation and Phosphorylation Induced by Lysophosphatidic Acid J. Biol. Chem., February 7, 2003; 278(7): 4882 - 4891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. McGrew, M. S. S. Chang, and E. Sanders-Bush Phospholipase D Activation by Endogenous 5-Hydroxytryptamine 2C Receptors Is Mediated by Galpha 13 and Pertussis Toxin-Insensitive Gbeta gamma Subunits Mol. Pharmacol., December 1, 2002; 62(6): 1339 - 1343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Park, N. T. Nguyen, P. Dutt, K. D. Merdek, M. Bashar, P. Sterpetti, A. Tosolini, J. R. Testa, and D. Toksoz Association of Lbc Rho Guanine Nucleotide Exchange Factor with alpha -Catenin-related Protein, alpha -Catulin/CTNNAL1, Supports Serum Response Factor Activation J. Biol. Chem., November 15, 2002; 277(47): 45361 - 45370. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Maruyama, M. Nishida, Y. Sugimoto, S. Tanabe, J. H. Turner, T. Kozasa, T. Wada, T. Nagao, and H. Kurose G{alpha}12/13 Mediates {alpha}1-Adrenergic Receptor-Induced Cardiac Hypertrophy Circ. Res., November 15, 2002; 91(10): 961 - 969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Gudi, J. C. Chen, D. E. Casteel, T. M. Seasholtz, G. R. Boss, and R. B. Pilz cGMP-dependent Protein Kinase Inhibits Serum-response Element-dependent Transcription by Inhibiting Rho Activation and Functions J. Biol. Chem., September 27, 2002; 277(40): 37382 - 37393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. J. Buchan, C.-Y. Lin, J. Choi, and D. L. Barber Somatostatin, Acting at Receptor Subtype 1, Inhibits Rho Activity, the Assembly of Actin Stress Fibers, and Cell Migration J. Biol. Chem., August 2, 2002; 277(32): 28431 - 28438. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Schmidt and A. Hall Guanine nucleotide exchange factors for Rho GTPases: turning on the switch Genes & Dev., July 1, 2002; 16(13): 1587 - 1609. [Full Text] [PDF]
|
|
|
|
|
|
T. E. Meigs, M. Fedor-Chaiken, D. D. Kaplan, R. Brackenbury, and P. J. Casey Galpha 12 and Galpha 13 Negatively Regulate the Adhesive Functions of Cadherin J. Biol. Chem., June 28, 2002; 277(27): 24594 - 24600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q.-D. NGUYEN, S. FAIVRE, E. BRUYNEEL, C. RIVAT, M. SETO, T. ENDO, M. MAREEL, S. EMAMI, and C. GESPACH RhoA- and RhoD-dependent regulatory switch of G{alpha} subunit signaling by PAR-1 receptors in cellular invasion FASEB J, April 1, 2002; 16(6): 565 - 576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Vaiskunaite, T. Kozasa, and T. A. Voyno-Yasenetskaya Interaction between the Galpha Subunit of Heterotrimeric G12 Protein and Hsp90 Is Required for Galpha 12 Signaling J. Biol. Chem., November 30, 2001; 276(49): 46088 - 46093. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yuan, L. W. Slice, and E. Rozengurt Activation of Protein Kinase D by Signaling through Rho and the alpha Subunit of the Heterotrimeric G Protein G13 J. Biol. Chem., October 12, 2001; 276(42): 38619 - 38627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Luo, L. R. Latchney, and D. J. Culp G protein coupling to M1 and M3 muscarinic receptors in sublingual glands Am J Physiol Cell Physiol, April 1, 2001; 280(4): C884 - C896. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Murthy, H. Zhou, J. R. Grider, and G. M. Makhlouf Sequential activation of heterotrimeric and monomeric G proteins mediates PLD activity in smooth muscle Am J Physiol Gastrointest Liver Physiol, March 1, 2001; 280(3): G381 - G388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bhattacharyya and P. B. Wedegaertner Galpha 13 Requires Palmitoylation for Plasma Membrane Localization, Rho-dependent Signaling, and Promotion of p115-RhoGEF Membrane Binding J. Biol. Chem., May 12, 2000; 275(20): 14992 - 14999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fukuhara, M. J. Marinissen, M. Chiariello, and J. S. Gutkind Signaling from G Protein-coupled Receptors to ERK5/Big MAPK 1 Involves Galpha q and Galpha 12/13 Families of Heterotrimeric G Proteins. EVIDENCE FOR THE EXISTENCE OF A NOVEL Ras AND Rho-INDEPENDENT PATHWAY J. Biol. Chem., July 7, 2000; 275(28): 21730 - 21736. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Even-Ram, M. Maoz, E. Pokroy, R. Reich, B.-Z. Katz, P. Gutwein, P. Altevogt, and R. Bar-Shavit Tumor Cell Invasion Is Promoted by Activation of Protease Activated Receptor-1 in Cooperation with the alpha vbeta 5 Integrin J. Biol. Chem., March 30, 2001; 276(14): 10952 - 10962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Sagi, T. M. Seasholtz, M. Kobiashvili, B. A. Wilson, D. Toksoz, and J. H. Brown Physical and Functional Interactions of Galpha q with Rho and Its Exchange Factors J. Biol. Chem., April 27, 2001; 276(18): 15445 - 15452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Siehler, Y. Wang, X. Fan, R. T. Windh, and D. R. Manning Sphingosine 1-Phosphate Activates Nuclear Factor-kappa B through Edg Receptors. ACTIVATION THROUGH Edg-3 AND Edg-5, BUT NOT Edg-1, IN HUMAN EMBRYONIC KIDNEY 293 CELLS J. Biol. Chem., December 21, 2001; 276(52): 48733 - 48739. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
