| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 38, 26869-26877, September 17, 1999
From the Department of Tumor Immunology, University Hospital
Nijmegen St. Radboud, 6525 EX Nijmegen, The Netherlands
To elucidate the role of the cytoskeleton
regulating avidity or affinity changes in the leukocyte adhesion
receptor lymphocyte function-associated antigen-1 (LFA-1)
(
L
2), we generated mutant cytoplasmic LFA-1 receptors and expressed these into the
erythroleukemic cell line K562. We determined whether intercellular
adhesion molecule-1 (ICAM-1)-mediated adhesion of LFA-1, lacking parts
of its cytoplasmic tails, is regulated through receptor
diffusion/clustering and/or by altered ligand binding affinity. All
cytoplasmic deletion mutants that lack the complete 2
cytoplasmic tail and/or the conserved KVGFFKR sequence in the
L cytoplasmic tail were constitutively active and
expressed high levels of the activation epitopes NKI-L16 and M24.
Surprisingly, whereas these mutants showed a clustered cell surface
distribution of LFA-1, the ligand-binding affinity as measured by
titration of soluble ligand ICAM-1 remained unaltered. The notion that
redistribution of LFA-1 does not alter ligand-binding affinity is
further supported by the finding that disruption of the cytoskeleton by
cytochalasin D did not alter the binding affinity nor adhesion to
ICAM-1 of these mutants. Most cytoplasmic deletion mutants that
spontaneously bound ICAM-1 were not capable to spread on ICAM-1,
demonstrating that on these mutants LFA-1 is not coupled to the actin
cytoskeleton. From these data we conclude that LFA-1-mediated cell
adhesion to ICAM-1 is predominantly regulated by receptor clustering
and that affinity alterations do not necessarily coincide with strong
ICAM-1 binding.
This article has been cited by other articles:
|
|
 |
|
  S. R. Thom, V. M. Bhopale, D. J. Mancini, and T. N. Milovanova Actin S-Nitrosylation Inhibits Neutrophil {beta}2 Integrin Function J. Biol. Chem., April 18, 2008; 283(16): 10822 - 10834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Chilcoat, Y. Sharief, and S. L. Jones Tonic protein kinase A activity maintains inactive {beta}2 integrins in unstimulated neutrophils by reducing myosin light-chain phosphorylation: role of myosin light-chain kinase and Rho kinase J. Leukoc. Biol., April 1, 2008; 83(4): 964 - 971. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. T. N. Simonson, S. J. Franco, and A. Huttenlocher Talin1 Regulates TCR-Mediated LFA-1 Function J. Immunol., December 1, 2006; 177(11): 7707 - 7714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Cambi, B. Joosten, M. Koopman, F. de Lange, I. Beeren, R. Torensma, J. A. Fransen, M. Garcia-Parajo, F. N. van Leeuwen, and C. G. Figdor Organization of the Integrin LFA-1 in Nanoclusters Regulates Its Activity Mol. Biol. Cell, October 1, 2006; 17(10): 4270 - 4281. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Koneru, N. Monu, D. Schaer, J. Barletta, and A. B. Frey Defective Adhesion in Tumor Infiltrating CD8+ T Cells J. Immunol., May 15, 2006; 176(10): 6103 - 6111. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. W. Zimmerman, B. Joosten, R. Torensma, J. R. Parnes, F. N. van Leeuwen, and C. G. Figdor Long-term engagement of CD6 and ALCAM is essential for T-cell proliferation induced by dendritic cells Blood, April 15, 2006; 107(8): 3212 - 3220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Ada-Nguema, H. Xenias, M. P. Sheetz, and P. J. Keely The small GTPase R-Ras regulates organization of actin and drives membrane protrusions through the activity of PLC{epsilon} J. Cell Sci., April 1, 2006; 119(7): 1307 - 1319. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Heit, P. Colarusso, and P. Kubes Fundamentally different roles for LFA-1, Mac-1 and {alpha}4-integrin in neutrophil chemotaxis J. Cell Sci., November 15, 2005; 118(22): 5205 - 5220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Tardif and M. J. Tremblay LFA-1 Is a Key Determinant for Preferential Infection of Memory CD4+ T Cells by Human Immunodeficiency Virus Type 1 J. Virol., November 1, 2005; 79(21): 13714 - 13724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Tardif and M. J. Tremblay Regulation of LFA-1 Activity through Cytoskeleton Remodeling and Signaling Components Modulates the Efficiency of HIV Type-1 Entry in Activated CD4+ T Lymphocytes J. Immunol., July 15, 2005; 175(2): 926 - 935. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B.-H. Luo, C. V. Carman, J. Takagi, and T. A. Springer Disrupting integrin transmembrane domain heterodimerization increases ligand binding affinity, not valency or clustering PNAS, March 8, 2005; 102(10): 3679 - 3684. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kim, C. V. Carman, W. Yang, A. Salas, and T. A. Springer The primacy of affinity over clustering in regulation of adhesiveness of the integrin {alpha}L{beta}2 J. Cell Biol., December 20, 2004; 167(6): 1241 - 1253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Lee, L. Del Sorbo, S. Uhlig, G. A. Porro, T. Whitehead, S. Voglis, M. Liu, A. S. Slutsky, and H. Zhang Intercellular Adhesion Molecule-1 Mediates Cellular Cross-Talk between Parenchymal and Immune Cells after Lipopolysaccharide Neutralization J. Immunol., January 1, 2004; 172(1): 608 - 616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Chigaev, T. Buranda, D. C. Dwyer, E. R. Prossnitz, and L. A. Sklar FRET Detection of Cellular {alpha}4-Integrin Conformational Activation Biophys. J., December 1, 2003; 85(6): 3951 - 3962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ni, C. G. Kevil, D. C. Bullard, and D. F. Kucik Avidity Modulation Activates Adhesion under Flow and Requires Cooperativity among Adhesion Receptors Biophys. J., December 1, 2003; 85(6): 4122 - 4133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Spaargaren, E. A. Beuling, M. L. Rurup, H. P. Meijer, M. D. Klok, S. Middendorp, R. W. Hendriks, and S. T. Pals The B Cell Antigen Receptor Controls Integrin Activity through Btk and PLC{gamma}2 J. Exp. Med., November 17, 2003; 198(10): 1539 - 1550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Giannoni, P. Chiarugi, G. Cozzi, L. Magnelli, M. L. Taddei, T. Fiaschi, F. Buricchi, G. Raugei, and G. Ramponi Lymphocyte Function-associated Antigen-1-mediated T Cell Adhesion Is Impaired by Low Molecular Weight Phosphotyrosine Phosphatase-dependent Inhibition of FAK Activity J. Biol. Chem., September 19, 2003; 278(38): 36763 - 36776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sanzone, M. Zeyda, M. D. Saemann, M. Soncini, W. Holter, G. Fritsch, W. Knapp, F. Candotti, T. M. Stulnig, and O. Parolini SLAM-associated Protein Deficiency Causes Imbalanced Early Signal Transduction and Blocks Downstream Activation in T Cells from X-linked Lymphoproliferative Disease Patients J. Biol. Chem., August 8, 2003; 278(32): 29593 - 29599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Willeke, J. Schymeinsky, P. Prange, S. Zahler, and B. Walzog A role for Syk-kinase in the control of the binding cycle of the {beta}2 integrins (CD11/CD18) in human polymorphonuclear neutrophils J. Leukoc. Biol., August 1, 2003; 74(2): 260 - 269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Marwali, J. Rey-Ladino, L. Dreolini, D. Shaw, and F. Takei Membrane cholesterol regulates LFA-1 function and lipid raft heterogeneity Blood, July 1, 2003; 102(1): 215 - 222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Tohyama, K. Katagiri, R. Pardi, C. Lu, T. A. Springer, and T. Kinashi The Critical Cytoplasmic Regions of the {alpha}L/{beta}2 Integrin in Rap1-induced Adhesion and Migration Mol. Biol. Cell, June 1, 2003; 14(6): 2570 - 2582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Worthylake and K. Burridge RhoA and ROCK Promote Migration by Limiting Membrane Protrusions J. Biol. Chem., April 4, 2003; 278(15): 13578 - 13584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Jaakkola, M. Merinen, S. Jalkanen, and A. Hanninen Ly6C Induces Clustering of LFA-1 (CD11a/CD18) and Is Involved in Subtype-Specific Adhesion of CD8 T Cells J. Immunol., February 1, 2003; 170(3): 1283 - 1290. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-B. Guo, I. Lee, M. Kamar, S. K. Akiyama, and M. Pierce Aberrant N-Glycosylation of {beta}1 Integrin Causes Reduced {alpha}5{beta}1 Integrin Clustering and Stimulates Cell Migration Cancer Res., December 1, 2002; 62(23): 6837 - 6845. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Liu, B. R. Schwartz, N. Lin, R. K. Winn, and J. M. Harlan Requirement for RhoA Kinase Activation in Leukocyte De-Adhesion J. Immunol., September 1, 2002; 169(5): 2330 - 2336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Jin and J. Li Dynamitin Controls beta 2 Integrin Avidity by Modulating Cytoskeletal Constraint on Integrin Molecules J. Biol. Chem., August 30, 2002; 277(36): 32963 - 32969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. T. de Bruyn, S. Rangarajan, K. A. Reedquist, C. G. Figdor, and J. L. Bos The Small GTPase Rap1 Is Required for Mn2+- and Antibody-induced LFA-1- and VLA-4-mediated Cell Adhesion J. Biol. Chem., August 9, 2002; 277(33): 29468 - 29476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. K. Griffiths and J. M. Penninger ADAP-ting TCR Signaling to Integrins Sci. Signal., April 9, 2002; 2002(127): re3 - re3. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Hyduk and M. I. Cybulsky {alpha}4 Integrin Signaling Activates Phosphatidylinositol 3-Kinase and Stimulates T Cell Adhesion to Intercellular Adhesion Molecule-1 to a Similar Extent As CD3, but Induces a Distinct Rearrangement of the Actin Cytoskeleton J. Immunol., January 15, 2002; 168(2): 696 - 704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Leitinger and N. Hogg The involvement of lipid rafts in the regulation of integrin function J. Cell Sci., January 3, 2002; 115(5): 963 - 972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Morgan, C. M. Labno, G. A. Van Seventer, M. F. Denny, D. B. Straus, and J. K. Burkhardt Superantigen-Induced T Cell:B Cell Conjugation Is Mediated by LFA-1 and Requires Signaling Through Lck, But Not ZAP-70 J. Immunol., November 15, 2001; 167(10): 5708 - 5718. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S.C. Weber, G. Ostermann, A. Zernecke, A. Schroder, L. B. Klickstein, and C. Weber Dual Role of H-Ras in Regulation of Lymphocyte Function Antigen-1 Activity by Stromal Cell-derived Factor-1alpha : Implications for Leukocyte Transmigration Mol. Biol. Cell, October 1, 2001; 12(10): 3074 - 3086. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Lupher Jr., E. A. S. Harris, C. R. Beals, L. Sui, R. C. Liddington, and D. E. Staunton Cellular Activation of Leukocyte Function-Associated Antigen-1 and Its Affinity Are Regulated at the I Domain Allosteric Site J. Immunol., August 1, 2001; 167(3): 1431 - 1439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Piccardoni, R. Sideri, S. Manarini, A. Piccoli, N. Martelli, G. de Gaetano, C. Cerletti, and V. Evangelista Platelet/polymorphonuclear leukocyte adhesion: a new role for SRC kinases in Mac-1 adhesive function triggered by P-selectin Blood, July 1, 2001; 98(1): 108 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Woods and Y. Shimizu Signaling networks regulating {beta}1 integrin-mediated adhesion of T lymphocytes to extracellular matrix J. Leukoc. Biol., June 1, 2001; 69(6): 874 - 880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Chan, S. J. Hyduk, and M. I. Cybulsky Chemoattractants Induce a Rapid and Transient Upregulation of Monocyte {alpha}4 Integrin Affinity for Vascular Cell Adhesion Molecule 1 Which Mediates Arrest: An Early Step in the Process of Emigration J. Exp. Med., May 14, 2001; 193(10): 1149 - 1158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Mistry, S. L. Cranmer, Y. Yuan, P. Mangin, S. M. Dopheide, I. Harper, S. Giuliano, D. E. Dunstan, F. Lanza, H. H. Salem, et al. Cytoskeletal regulation of the platelet glycoprotein Ib/V/IX-von Willebrand factor interaction Blood, November 15, 2000; 96(10): 3480 - 3489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-M. Tan, R. H. Hyland, A. Al-Shamkhani, W. A. Douglass, J. M. Shaw, and S. K. A. Law Effect of Integrin {beta}2 Subunit Truncations on LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) Assembly, Surface Expression, and Function J. Immunol., September 1, 2000; 165(5): 2574 - 2581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sigal, D. A. Bleijs, V. Grabovsky, S. J. van Vliet, O. Dwir, C. G. Figdor, Y. van Kooyk, and R. Alon The LFA-1 Integrin Supports Rolling Adhesions on ICAM-1 Under Physiological Shear Flow in a Permissive Cellular Environment J. Immunol., July 1, 2000; 165(1): 442 - 452. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-P. Xiong, T. Stehle, B. Diefenbach, R. Zhang, R. Dunker, D. L. Scott, A. Joachimiak, S. L. Goodman, and M. A. Arnaout Crystal Structure of the Extracellular Segment of Integrin alpha Vbeta 3 Science, October 12, 2001; 294(5541): 339 - 345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wang, H. Chen, and E. J. Brown L-plastin Peptide Activation of alpha vbeta 3-mediated Adhesion Requires Integrin Conformational Change and Actin Filament Disassembly J. Biol. Chem., April 20, 2001; 276(17): 14474 - 14481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Bleijs, G. C. F. van Duijnhoven, S. J. van Vliet, J. P. H. Thijssen, C. G. Figdor, and Y. van Kooyk A Single Amino Acid in the Cytoplasmic Domain of the beta 2 Integrin Lymphocyte Function-associated Antigen-1 Regulates Avidity-dependent Inside-out Signaling J. Biol. Chem., March 23, 2001; 276(13): 10338 - 10346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Dierks, J. Kolanus, and W. Kolanus Actin Cytoskeletal Association of Cytohesin-1 Is Regulated by Specific Phosphorylation of Its Carboxyl-terminal Polybasic Domain J. Biol. Chem., September 28, 2001; 276(40): 37472 - 37481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Rodriguez-Fernandez, L. Sanchez-Martin, M. Rey, M. Vicente-Manzanares, S. Narumiya, J. Teixido, F. Sanchez-Madrid, and C. Cabanas Rho and Rho-associated Kinase Modulate the Tyrosine Kinase PYK2 in T-cells through Regulation of the Activity of the Integrin LFA-1 J. Biol. Chem., October 26, 2001; 276(44): 40518 - 40527. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhou, J. Li, and D. F. Kucik The Microtubule Cytoskeleton Participates in Control of beta 2 Integrin Avidity J. Biol. Chem., November 21, 2001; 276(48): 44762 - 44769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fagerholm, N. Morrice, C. G. Gahmberg, and P. Cohen Phosphorylation of the Cytoplasmic Domain of the Integrin CD18 Chain by Protein Kinase C Isoforms in Leukocytes J. Biol. Chem., January 11, 2002; 277(3): 1728 - 1738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. Harris, T. M. McIntyre, S. M. Prescott, and G. A. Zimmerman The Leukocyte Integrins J. Biol. Chem., July 28, 2000; 275(31): 23409 - 23412. [Full Text] [PDF]
|
|
|
|
|
|
M. Shimaoka, C. Lu, R. T. Palframan, U. H. von Andrian, A. McCormack, J. Takagi, and T. A. Springer Reversibly locking a protein fold in an active conformation with a disulfide bond: Integrin alpha L I domains with high affinity and antagonist activity in vivo PNAS, May 22, 2001; 98(11): 6009 - 6014. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
