HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hocking, D. C. Articles by McKeown-Longo, P. J. Search for Related Content PubMed PubMed Citation Articles by Hocking, D. C. Articles by McKeown-Longo, P. J. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 38, 27257-27264, September 17, 1999

Inhibition of Fibronectin Matrix Assembly by the Heparin-binding Domain of Vitronectin

From the Cell and Molecular Biology Program and the Department of Physiology and Cell Biology, Albany Medical College, Albany, New York 12208 and the ^§ Department of Experimental Pathology, Lund University, 22185 Lund, Sweden

The deposition of fibronectin into the extracellular matrix is an integrin-dependent, multistep process that is tightly regulated in order to ensure controlled matrix deposition. Reduced fibronectin deposition has been associated with altered embryonic development, tumor cell invasion, and abnormal wound repair. In one of the initial steps of fibronectin matrix assembly, the amino-terminal region of fibronectin binds to cell surface receptors, termed matrix assembly sites. The present study was undertaken to investigate the role of extracellular signals in the regulation of fibronectin deposition. Our data indicate that the interaction of cells with the extracellular glycoprotein, vitronectin, specifically inhibits matrix assembly site expression and fibronectin deposition. The region of vitronectin responsible for the inhibition of fibronectin deposition was localized to the heparin-binding domain. Vitronectin's heparin-binding domain inhibited both ₁ and non-₁ integrin-dependent matrix assembly site expression and could be overcome by treatment of cells with lysophosphatidic acid, an agent that promotes actin polymerization. The interaction of cells with the heparin-binding domain of vitronectin resulted in changes in actin microfilament organization and the subcellular distribution of the actin-associated proteins -actinin and talin. These data suggest a mechanism whereby the heparin-binding domain of vitronectin regulates the deposition of fibronectin into the extracellular matrix through alterations in the organization of the actin cytoskeleton.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				D. Vial and P. J. McKeown-Longo PAI1 stimulates assembly of the fibronectin matrix in osteosarcoma cells through crosstalk between the {alpha}v{beta}5 and {alpha}5{beta}1 integrins J. Cell Sci., May 15, 2008; 121(10): 1661 - 1670. [Abstract] [Full Text] [PDF]

				E. Bae, T. Sakai, and D. F. Mosher Assembly of Exogenous Fibronectin by Fibronectin-null Cells Is Dependent on the Adhesive Substrate J. Biol. Chem., August 20, 2004; 279(34): 35749 - 35759. [Abstract] [Full Text] [PDF]

				E. Monaghan, V. Gueorguiev, C. Wilkins-Port, and P. J. McKeown-Longo The Receptor for Urokinase-type Plasminogen Activator Regulates Fibronectin Matrix Assembly in Human Skin Fibroblasts J. Biol. Chem., January 9, 2004; 279(2): 1400 - 1407. [Abstract] [Full Text] [PDF]

				J. A. Kricker, C. L. Towne, S. M. Firth, A. C. Herington, and Z. Upton Structural and Functional Evidence for the Interaction of Insulin-Like Growth Factors (IGFs) and IGF Binding Proteins with Vitronectin Endocrinology, July 1, 2003; 144(7): 2807 - 2815. [Abstract] [Full Text] [PDF]

				D. C. Hocking and K. Kowalski A cryptic fragment from fibronectin's III1 module localizes to lipid rafts and stimulates cell growth and contractility J. Cell Biol., July 8, 2002; 158(1): 175 - 184. [Abstract] [Full Text] [PDF]

				Y. Lim, I. Han, H. J. Kwon, and E.-S. Oh Trichostatin A-induced Detransformation Correlates with Decreased Focal Adhesion Kinase Phosphorylation at Tyrosine 861 in ras-transformed Fibroblasts J. Biol. Chem., April 5, 2002; 277(15): 12735 - 12740. [Abstract] [Full Text] [PDF]

				M. Pereira, B. J. Rybarczyk, T. M. Odrljin, D. C. Hocking, J. Sottile, and P. J. Simpson-Haidaris The incorporation of fibrinogen into extracellular matrix is dependent on active assembly of a fibronectin matrix J. Cell Sci., January 2, 2002; 115(3): 609 - 617. [Abstract] [Full Text] [PDF]

				J Sottile, D. Hocking, and K. Langenbach Fibronectin polymerization stimulates cell growth by RGD-dependent and -independent mechanisms J. Cell Sci., January 12, 2000; 113(23): 4287 - 4299. [Abstract] [PDF]