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J Biol Chem, Vol. 274, Issue 39, 27590-27596, September 24, 1999

Oxidative Stress and Iron Are Implicated in Fragmenting Vacuoles of Saccharomyces cerevisiae Lacking Cu,Zn-Superoxide Dismutase

Laura B. CorsonDagger §, Janet Folmer, Jeffrey J. Strain, Valeria C. Culotta, and Don W. Cleveland§parallel

From the Dagger  Predoctoral Program in Human Genetics, Johns Hopkins University, Baltimore, Maryland 21205, the § Ludwig Institute for Cancer Research, La Jolla, California 92093, the  Department of Environmental Health Sciences, Johns Hopkins School of Hygiene and Public Health, Baltimore, Maryland 21205, and the parallel  Departments of Medicine and Neuroscience, University of California at San Diego, La Jolla, California 92093

The absence of the antioxidant enzyme Cu,Zn-superoxide dismutase (SOD1) is shown here to cause vacuolar fragmentation in Saccharomyces cerevisiae. Wild-type yeast have 1-3 large vacuoles whereas the sod1Delta yeast have as many as 50 smaller vacuoles. Evidence that this fragmentation is oxygen-mediated includes the findings that aerobically (but not anaerobically) grown sod1Delta yeast exhibit aberrant vacuoles and genetic suppressors of other oxygen-dependent sod1 null phenotypes rescue the vacuole defect. Surprisingly, iron also is implicated in the fragmentation process as iron addition exacerbates the sod1Delta vacuole defect while iron starvation ameliorates it. Because the vacuole is reported to be a site of iron storage and iron reacts avidly with reactive oxygen species to generate toxic side products, we propose that vacuole damage in sod1Delta cells arises from an elevation of iron-mediated oxidation within the vacuole or from elevated pools of "free" iron that may bind nonproductively to vacuolar ligands. Furthermore, additional pleiotropic phenotypes of sod1Delta cells (including increased sensitivity to pH, nutrient deprivation, and metals) may be secondary to vacuolar compromise. Our findings support the hypothesis that oxidative stress alters cellular iron homeostasis which in turn increases oxidative damage. Thus, our findings may have medical relevance as both oxidative stress and alterations in iron homeostasis have been implicated in diverse human disease processes. Our findings suggest that strategies to decrease intracellular iron may significantly reduce oxidatively induced cellular damage.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.


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