| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 39, 27867-27874, September 24, 1999
,,,
,,,,
From the We report the cloning, expression, and
characterization of a novel member of the mammalian
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase (ppGaNTase) family that transfers GalNAc to a GalNAc-containing glycopeptide. Northern blot analysis revealed that the gene encoding this enzyme, termed ppGaNTase-T6, is expressed in a highly
tissue-specific manner. Significant levels of transcript were found in
rat and mouse sublingual gland, stomach, small intestine, and colon;
trace amounts were seen in the ovary, cervix, and uterus. Recombinant constructs were expressed transiently in COS7 cells but demonstrated no
transferase activity in vitro against a panel of unmodified peptides, including GTTPSPVPTTSTTSAP (MUC5AC). However, when incubated with the total glycosylated products obtained by action of ppGaNTase-T1 on MUC5AC (mainly GTT(GalNAc)PSPVPTTSTT(GalNAc)SAP), additional incorporation of GalNAc was achieved, resulting in new hydroxyamino acids being modified. The MUC5AC glycopeptide failed to serve as a
substrate for ppGaNTase-T6 after modification of the GalNAc residues by
periodate oxidation and sodium borohydride reduction, indicating a
requirement for the presence of intact GalNAc. This suggests that
O-glycosylation of multisite substrates may proceed in a
specific hierarchical manner and underscores the potential complexity
of the processes that regulate O-glycosylation.
Center for Oral Biology, Rochester Institute
of Biomedical Sciences, University of Rochester,
Rochester, New York 14642, ¶ Unité INSERM 377, Biologie
et Physiopathologie de Cellules Mucipares, Place de Verdun,
59045 Lille Cédex, France, and Institut de Biologie
Structurale JP EBEL, CEA/CNRS, 41 Avenue de Martyrs,
38027 Grenoble Cédex 1, France
This article has been cited by other articles:
|
|
 |
|
  M. Tenno, K. Ohtsubo, F. K. Hagen, D. Ditto, A. Zarbock, P. Schaerli, U. H. von Andrian, K. Ley, D. Le, L. A. Tabak, et al. Initiation of Protein O Glycosylation by the Polypeptide GalNAcT-1 in Vascular Biology and Humoral Immunity Mol. Cell. Biol., December 15, 2007; 27(24): 8783 - 8796. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. H. Wandall, F. Irazoqui, M. A. Tarp, E. P. Bennett, U. Mandel, H. Takeuchi, K. Kato, T. Irimura, G. Suryanarayanan, M. A. Hollingsworth, et al. The lectin domains of polypeptide GalNAc-transferases exhibit carbohydrate-binding specificity for GalNAc: lectin binding to GalNAc-glycopeptide substrates is required for high density GalNAc-O-glycosylation Glycobiology, April 1, 2007; 17(4): 374 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Gerken, J. Raman, T. A. Fritz, and O. Jamison Identification of Common and Unique Peptide Substrate Preferences for the UDP-GalNAc:Polypeptide {alpha}-N-acetylgalactosaminyltransferases T1 and T2 Derived from Oriented Random Peptide Substrates J. Biol. Chem., October 27, 2006; 281(43): 32403 - 32416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Julenius, A. Molgaard, R. Gupta, and S. Brunak Prediction, conservation analysis, and structural characterization of mammalian mucin-type O-glycosylation sites Glycobiology, February 1, 2005; 15(2): 153 - 164. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Fritz, J. H. Hurley, L.-B. Trinh, J. Shiloach, and L. A. Tabak The beginnings of mucin biosynthesis: The crystal structure of UDP-GalNAc:polypeptide {alpha}-N-acetylgalactosaminyltransferase-T1 PNAS, October 26, 2004; 101(43): 15307 - 15312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. G. Ten Hagen, D. T. Tran, T. A. Gerken, D. S. Stein, and Z. Zhang Functional Characterization and Expression Analysis of Members of the UDP-GalNAc:Polypeptide N-Acetylgalactosaminyltransferase Family from Drosophila melanogaster J. Biol. Chem., September 12, 2003; 278(37): 35039 - 35048. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. W. Young Jr., D. R. Holcomb, K. G. Ten Hagen, and L. A. Tabak Expression of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase isoforms in murine tissues determined by real-time PCR: a new view of a large family Glycobiology, July 1, 2003; 13(7): 549 - 557. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. T. Marcos, A. Cruz, F. Silva, R. Almeida, L. David, U. Mandel, H. Clausen, S. von Mensdorff-Pouilly, and C. A. Reis Polypeptide GalNAc-transferases, ST6GalNAc-transferase I, and ST3Gal-transferase I Expression in Gastric Carcinoma Cell Lines J. Histochem. Cytochem., June 1, 2003; 51(6): 761 - 771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Iwasaki, Y. Zhang, K. Tachibana, M. Gotoh, N. Kikuchi, Y.-D. Kwon, A. Togayachi, T. Kudo, T. Kubota, and H. Narimatsu Initiation of O-Glycan Synthesis in IgA1 Hinge Region Is Determined by a Single Enzyme, UDP-N-Acetyl-alpha -D-galactosamine:Polypeptide N-Acetylgalactosaminyltransferase 2 J. Biol. Chem., February 14, 2003; 278(8): 5613 - 5621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, H. Iwasaki, H. Wang, T. Kudo, T. B. Kalka, T. Hennet, T. Kubota, L. Cheng, N. Inaba, M. Gotoh, et al. Cloning and Characterization of a New Human UDP-N-Acetyl-alpha -D-galactosamine:Polypeptide N-Acetylgalactosaminyltransferase, Designated pp-GalNAc-T13, That Is Specifically Expressed in Neurons and Synthesizes GalNAc alpha -Serine/Threonine Antigen J. Biol. Chem., January 3, 2003; 278(1): 573 - 584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. G. Ten Hagen, T. A. Fritz, and L. A. Tabak All in the family: the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases Glycobiology, January 1, 2003; 13(1): 1R - 16R. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Gerken, J. Zhang, J. Levine, and A. Elhammer Mucin Core O-Glycosylation Is Modulated by Neighboring Residue Glycosylation Status. KINETIC MODELING OF THE SITE-SPECIFIC GLYCOSYLATION OF THE APO-PORCINE SUBMAXILLARY MUCIN TANDEM REPEAT BY UDP-GalNAc:POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASES T1 AND T2 J. Biol. Chem., December 13, 2002; 277(51): 49850 - 49862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tenno, A. Saeki, F. J. Kezdy, A. P. Elhammer, and A. Kurosaka The Lectin Domain of UDP-GalNAc:Polypeptide N-Acetylgalactosaminyltransferase 1 Is Involved in O-Glycosylation of a Polypeptide with Multiple Acceptor Sites J. Biol. Chem., November 27, 2002; 277(49): 47088 - 47096. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. G. T. Hagen and D. T. Tran A UDP-GalNAc:Polypeptide N-Acetylgalactosaminyltransferase Is Essential for Viability in Drosophila melanogaster J. Biol. Chem., June 14, 2002; 277(25): 22616 - 22622. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Schwientek, E. P. Bennett, C. Flores, J. Thacker, M. Hollmann, C. A. Reis, J. Behrens, U. Mandel, B. Keck, M. A. Schafer, et al. Functional Conservation of Subfamilies of Putative UDP-N-acetylgalactosamine:Polypeptide N-Acetylgalactosaminyltransferases in Drosophila, Caenorhabditis elegans, and Mammals. ONE SUBFAMILY COMPOSED OF l(2)35Aa IS ESSENTIAL IN DROSOPHILA J. Biol. Chem., June 14, 2002; 277(25): 22623 - 22638. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Gerken, M. Gilmore, and J. Zhang Determination of the Site-specific Oligosaccharide Distribution of the O-Glycans Attached to the Porcine Submaxillary Mucin Tandem Repeat. FURTHER EVIDENCE FOR THE MODULATION OF O-GLYCAN SIDE CHAIN STRUCTURES BY PEPTIDE SEQUENCE J. Biol. Chem., March 1, 2002; 277(10): 7736 - 7751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kato, H. Takeuchi, A. Kanoh, U. Mandel, H. Hassan, H. Clausen, and T. Irimura N-acetylgalactosamine incorporation into a peptide containing consecutive threonine residues by UDP-N-acetyl-D-galactosaminide:polypeptide N-acetylgalactosaminyltransferases Glycobiology, October 1, 2001; 11(10): 821 - 829. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Nealen, K. V. Vijayan, E. Bolton, and P. F. Bray Human Platelets Contain a Glycosylated Estrogen Receptor {beta} Circ. Res., March 2, 2001; 88(4): 438 - 442. [Abstract] [Full Text]
|
|
|
|
|
|
M. Biller, K. Mardberg, H. Hassan, H. Clausen, A. Bolmstedt, T. Bergstrom, and S. Olofsson Early steps in O-linked glycosylation and clustered O-linked glycans of herpes simplex virus type 1 glycoprotein C: effects on glycoprotein properties Glycobiology, December 1, 2000; 10(12): 1259 - 1269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. C. M. Brinkman-Van der Linden and A. Varki New Aspects of Siglec Binding Specificities, Including the Significance of Fucosylation and of the Sialyl-Tn Epitope J. Biol. Chem., March 17, 2000; 275(12): 8625 - 8632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ujita, A. K. Misra, J. McAuliffe, O. Hindsgaul, and M. Fukuda Poly-N-acetyllactosamine Extension in N-Glycans and Core 2- and Core 4-branched O-Glycans Is Differentially Controlled by i-Extension Enzyme and Different Members of the beta 1,4-Galactosyltransferase Gene Family J. Biol. Chem., May 19, 2000; 275(21): 15868 - 15875. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hassan, C. A. Reis, E. P. Bennett, E. Mirgorodskaya, P. Roepstorff, M. A. Hollingsworth, J. Burchell, J. Taylor-Papadimitriou, and H. Clausen The Lectin Domain of UDP-N-acetyl-D-galactosamine:Polypeptide N-acetylgalactosaminyltransferase-T4 Directs Its Glycopeptide Specificities J. Biol. Chem., December 1, 2000; 275(49): 38197 - 38205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. G. T. Hagen, G. S. Bedi, D. Tetaert, P. D. Kingsley, F. K. Hagen, M. M. Balys, T. M. Beres, P. Degand, and L. A. Tabak Cloning and Characterization of a Ninth Member of the UDP-GalNAc:Polypeptide N-Acetylgalactosaminyltransferase Family, ppGaNTase-T9 J. Biol. Chem., May 11, 2001; 276(20): 17395 - 17404. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
