|
J Biol Chem, Vol. 274, Issue 39, 27925-27933, September 24, 1999
Apolipoprotein A-I Stimulates Secretion of Apolipoprotein E
by Foam Cell Macrophages
David
Rees ,
Timothy
Sloane¶,
Wendy
Jessup ,
Roger T.
Dean , and
Leonard
Kritharides¶
From the Cell Biology and ¶ Clinical Research
Groups, Heart Research Institute, 145 Missenden Road, Camperdown,
Sydney, New South Wales 2050 and the
 Department of Cardiology, Concord Hospital,
University of Sydney, Hospital Road, Concord, Sydney,
New South Wales 2139, Australia
Apolipoprotein A-I (apoA-I) overexpression
inhibits atherogenesis in mice, and apolipoprotein E (apoE) secreted by
foam cell macrophages may exert antiatherogenic effects within the
arterial wall. We hypothesized that interaction between apoA-I and apoE contributed to the antiatherogenic properties of apoA-I, and therefore investigated whether apoA-I stimulated secretion of apoE by foam cell
macrophages. Cholesterol enrichment of primary murine and human
macrophages increased spontaneous apoE secretion 2-fold, as quantified
by Western blot and chemiluminescence detection. Human apoA-I caused a
further marked increase of apoE secretion from both murine (3.8-fold,
p < 0.01) and human (3.2-fold, p = 0.01) foam cells in a time- and concentration- dependent manner, and
this increase was confirmed by immunoprecipitation of
[35S]methionine-labeled macrophage apoE. The protein
synthesis inhibitor cycloheximide, but not the transcription inhibitor
actinomycin D, markedly inhibited apoE secretion to apoA-I (73.1 ± 9.8% inhibition at 4 h) and completely suppressed apoE
secretion beyond 4 h. Pretreatment of macrophages with Pronase
inhibited initial apoA-I-mediated apoE secretion by 70.5 ± 6.5%
at 2 h, but by 8 h apoA-I-induced apoE secretion was the same
in Pronase-pretreated and non-pretreated cells.
Non-apolipoprotein-mediated cholesterol efflux induced by trimethyl-
cyclodextrin did not enhance apoE secretion, whereas phospholipid
vesicles inducing the same degree of cholesterol efflux substantially
enhanced apoE secretion, and apoA-I and phospholipid vesicles in
combination demonstrated additive induction of apoE secretion. We
conclude that apoA-I concurrently stimulates apoE secretion and
cholesterol efflux from foam cell macrophages and that
lipoprotein-derived apoA-I may enhance local secretion and accumulation
of apoE in atherosclerotic lesions.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.

CiteULike Complore Connotea Del.icio.us Digg Reddit Technorati What's this?
This article has been cited by other articles:

|
 |

|
 |
 
K. R. Bales, F. Liu, S. Wu, S. Lin, D. Koger, C. DeLong, J. C. Hansen, P. M. Sullivan, and S. M. Paul
Human APOE Isoform-Dependent Effects on Brain {beta}-Amyloid Levels in PDAPP Transgenic Mice
J. Neurosci.,
May 27, 2009;
29(21):
6771 - 6779.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Kockx, W. Jessup, and L. Kritharides
Regulation of Endogenous Apolipoprotein E Secretion by Macrophages
Arterioscler. Thromb. Vasc. Biol.,
June 1, 2008;
28(6):
1060 - 1067.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Kockx, D. L. Guo, T. Huby, P. Lesnik, J. Kay, T. Sabaretnam, E. Jary, M. Hill, K. Gaus, J. Chapman, et al.
Secretion of Apolipoprotein E From Macrophages Occurs via a Protein Kinase A and Calcium-Dependent Pathway Along the Microtubule Network
Circ. Res.,
September 14, 2007;
101(6):
607 - 616.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
I. C. Gelissen, M. Harris, K.-A. Rye, C. Quinn, A. J. Brown, M. Kockx, S. Cartland, M. Packianathan, L. Kritharides, and W. Jessup
ABCA1 and ABCG1 Synergize to Mediate Cholesterol Export to ApoA-I
Arterioscler. Thromb. Vasc. Biol.,
March 1, 2006;
26(3):
534 - 540.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. H. Hasty, M. R. Plummer, K. H. Weisgraber, M. F. Linton, S. Fazio, and L. L. Swift
The recycling of apolipoprotein E in macrophages: influence of HDL and apolipoprotein A-I
J. Lipid Res.,
July 1, 2005;
46(7):
1433 - 1439.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. S. Spagnuolo, L. Cigliano, L. D. D'Andrea, C. Pedone, and P. Abrescia
Assignment of the Binding Site for Haptoglobin on Apolipoprotein A-I
J. Biol. Chem.,
January 14, 2005;
280(2):
1193 - 1198.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. Heeren, T. Grewal, A. Laatsch, N. Becker, F. Rinninger, K.-A. Rye, and U. Beisiegel
Impaired Recycling of Apolipoprotein E4 Is Associated with Intracellular Cholesterol Accumulation
J. Biol. Chem.,
December 31, 2004;
279(53):
55483 - 55492.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Kockx, K.-A. Rye, K. Gaus, C. M. Quinn, J. Wright, T. Sloane, D. Sviridov, Y. Fu, D. Sullivan, J. R. Burnett, et al.
Apolipoprotein A-I-stimulated Apolipoprotein E Secretion from Human Macrophages Is Independent of Cholesterol Efflux
J. Biol. Chem.,
June 18, 2004;
279(25):
25966 - 25977.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. Lee, A. Tauscher, D. W. Seo, J. F. Oram, and R. Kuver
Cultured gallbladder epithelial cells synthesize apolipoproteins A-I and E
Am J Physiol Gastrointest Liver Physiol,
August 8, 2003;
285(3):
G630 - G641.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. M. Liu, A. Cogny, M. Kockx, R. T. Dean, K. Gaus, W. Jessup, and L. Kritharides
Cyclodextrins differentially mobilize free and esterified cholesterol from primary human foam cell macrophages
J. Lipid Res.,
June 1, 2003;
44(6):
1156 - 1166.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. Heeren, T. Grewal, A. Laatsch, D. Rottke, F. Rinninger, C. Enrich, and U. Beisiegel
Recycling of Apoprotein E Is Associated with Cholesterol Efflux and High Density Lipoprotein Internalization
J. Biol. Chem.,
April 11, 2003;
278(16):
14370 - 14378.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. H. Farkas, L. L. Swift, A. H. Hasty, M. F. Linton, and S. Fazio
The Recycling of Apolipoprotein E in Primary Cultures of Mouse Hepatocytes. EVIDENCE FOR A PHYSIOLOGIC CONNECTION TO HIGH DENSITY LIPOPROTEIN METABOLISM
J. Biol. Chem.,
March 7, 2003;
278(11):
9412 - 9417.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. P. Vallely, P. G. Bannon, C. F. Hughes, and L. Kritharides
Endothelial expression of intercellular adhesion molecule 1 and vascular cell adhesion molecule 1 is suppressed by postbypass plasma containing increased soluble intercellular adhesion molecule 1 and vascular cell adhesion molecule 1
J. Thorac. Cardiovasc. Surg.,
October 1, 2002;
124(4):
758 - 767.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Bultel-Brienne, S. Lestavel, A. Pilon, I. Laffont, A. Tailleux, J.-C. Fruchart, G. Siest, and V. Clavey
Lipid Free Apolipoprotein E Binds to the Class B Type I Scavenger Receptor I (SR-BI) and Enhances Cholesteryl Ester Uptake from Lipoproteins
J. Biol. Chem.,
September 20, 2002;
277(39):
36092 - 36099.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. VON ECKARDSTEIN, C. LANGER, T. ENGEL, I. SCHAUKAL, A. CIGNARELLA, J. REINHARDT, S. LORKOWSKI, Z. LI, X. ZHOU, P. CULLEN, et al.
ATP binding cassette transporter ABCA1 modulates the secretion of apolipoprotein E from human monocyte-derived macrophages
FASEB J,
July 1, 2001;
15(9):
1555 - 1561.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. von Eckardstein, J.-R. Nofer, and G. Assmann
High Density Lipoproteins and Arteriosclerosis : Role of Cholesterol Efflux and Reverse Cholesterol Transport
Arterioscler. Thromb. Vasc. Biol.,
January 1, 2001;
21(1):
13 - 27.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. J. Brown, G. F. Watts, J. R. Burnett, R. T. Dean, and W. Jessup
Sterol 27-Hydroxylase Acts on 7-Ketocholesterol in Human Atherosclerotic Lesions and Macrophages in Culture
J. Biol. Chem.,
September 1, 2000;
275(36):
27627 - 27633.
[Abstract]
[Full Text]
[PDF]
|
 |
|
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.
|
Advertisement
Advertisement
|