| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 4, 1920-1927, January 22, 1999
Subunit and Is Responsible for the
Decreased Activity in Epithelial Cells
,
,
From the Department of Molecular Medicine, Karolinska Institutet,
The Rolf Luft Center for Diabetes Research, Karolinska Hospital, S-171
76 Stockholm, Sweden, the Dopamine inhibits
Na+,K+-ATPase activity in renal tubule
cells. This inhibition is associated with phosphorylation and
internalization of the We conclude that dopamine-induced inhibition of
Na+,K+-ATPase activity in rat renal tubule
cells requires endocytosis of the Department of Pharmacological
and Pharmaceutical Sciences, College of Pharmacy, University of
Houston, Houston, Texas 77204, the § Department of
Physiology, Texas Tech University, Lubbock, Texas 79430, the
¶ Department of Medicine, University of Chicago, Chicago, Illinois
60637, and the Division de Néphrologie, Hôpital
Cantonal Universitaire, CH-1211 Geneva 14, Switzerland
subunit, both events being protein kinase
C-dependent. Studies of purified preparations, fusion
proteins with site-directed mutagenesis, and heterologous expression
systems have identified two major protein kinase C phosphorylation
residues (Ser-11 and Ser-18) in the rat 1 subunit
isoform. To identify the phosphorylation site(s) that mediates
endocytosis of the subunit in response to dopamine, we have performed
site-directed mutagenesis of these residues in the rat 1
subunit and expressed the mutated forms in a renal epithelial cell
line. Dopamine inhibited Na+,K+-ATPase activity
and increased subunit phosphorylation and
clathrin-dependent endocytosis into endosomes in cells
expressing the wild type 1 subunit or the S11A
1 mutant, and both effects were blocked by protein
kinase C inhibition. In contrast, dopamine did not elicit any of these
effects in cells expressing the S18A 1 mutant. While Ser-18 phosphorylation is necessary for endocytosis, it does not affect
per se the enzymatic activity: preventing endocytosis with wortmannin or LY294009 blocked the inhibitory effect of dopamine on
Na+,K+-ATPase activity, although it did not
alter the increased subunit phosphorylation induced by this agonist.
subunit into defined
intracellular compartments and that phosphorylation of Ser-18 is
essential for this process.
This article has been cited by other articles:
|
|
 |
|
  H. Cai, L. Wu, W. Qu, D. Malhotra, Z. Xie, J. I. Shapiro, and J. Liu Regulation of apical NHE3 trafficking by ouabain-induced activation of the basolateral Na+-K+-ATPase receptor complex Am J Physiol Cell Physiol, February 1, 2008; 294(2): C555 - C563. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kimura, P. B. Allen, A. C. Nairn, and M. J. Caplan Arrestins and Spinophilin Competitively Regulate Na+,K+-ATPase Trafficking through Association with a Large Cytoplasmic Loop of the Na+,K+-ATPase Mol. Biol. Cell, November 1, 2007; 18(11): 4508 - 4518. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sjostrom, K. Stenstrom, K. Eneling, J. Zwiller, A. I. Katz, H. Takemori, and A. M. Bertorello SIK1 is part of a cell sodium-sensing network that regulates active sodium transport through a calcium-dependent process PNAS, October 23, 2007; 104(43): 16922 - 16927. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Schoner and G. Scheiner-Bobis Endogenous and exogenous cardiac glycosides: their roles in hypertension, salt metabolism, and cell growth Am J Physiol Cell Physiol, August 1, 2007; 293(2): C509 - C536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Kirchheimer, C. F. Mendez, A. Acquier, and S. Nowicki Role of 20-HETE in D1/D2 dopamine receptor synergism resulting in the inhibition of Na+-K+-ATPase activity in the proximal tubule Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1435 - F1442. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Lecuona, L. A. Dada, H. Sun, M. L. Butti, G. Zhou, T.-L. Chew, and J. I. Sznajder Na,K-ATPase {alpha}1-subunit dephosphorylation by protein phosphatase 2A is necessary for its recruitment to the plasma membrane FASEB J, December 1, 2006; 20(14): 2618 - 2620. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Collawn Unlocking the Mysteries of Na+-K+-ATPase Endocytosis: Phosphorylation Is the Key. Am. J. Respir. Cell Mol. Biol., July 1, 2006; 35(1): 1 - 2. [Full Text] [PDF]
|
|
|
|
|
|
Z. Chen, R. T. Krmar, L. Dada, R. Efendiev, I. B. Leibiger, C. H. Pedemonte, A. I. Katz, J. I. Sznajder, and A. M. Bertorello Phosphorylation of Adaptor Protein-2 {micro}2 Is Essential for Na+,K+-ATPase Endocytosis in Response to Either G Protein-Coupled Receptor or Reactive Oxygen Species Am. J. Respir. Cell Mol. Biol., July 1, 2006; 35(1): 127 - 132. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Song, M. Y. Lee, S. P. Kinsey, D. J. Weber, and M. P. Blaustein An N-terminal Sequence Targets and Tethers Na+ Pump {alpha}2 Subunits to Specialized Plasma Membrane Microdomains J. Biol. Chem., May 5, 2006; 281(18): 12929 - 12940. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Efendiev and C. H. Pedemonte Contrary to Rat-Type, Human-Type Na,K-ATPase Is Phosphorylated at the Same Amino Acid by Hormones that Produce Opposite Effects on Enzyme Activity J. Am. Soc. Nephrol., January 1, 2006; 17(1): 31 - 38. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Bianchi Genetic variations of tubular sodium reabsorption leading to "primary" hypertension: from gene polymorphism to clinical symptoms Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2005; 289(6): R1536 - R1549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Bertorello and J. I. Sznajder The Dopamine Paradox in Lung and Kidney Epithelia: Sharing the Same Target but Operating Different Signaling Networks Am. J. Respir. Cell Mol. Biol., November 1, 2005; 33(5): 432 - 437. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Ebensperger, R. Ebensperger, E. A Herrera, R. A Riquelme, E. M Sanhueza, F. Lesage, J. J Marengo, R. I Tejo, A. J Llanos, and R. V Reyes Fetal brain hypometabolism during prolonged hypoxaemia in the llama J. Physiol., September 15, 2005; 567(3): 963 - 975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M Woollhead, J. W Scott, D. G. Hardie, and D. L Baines Phenformin and 5-aminoimidazole-4-carboxamide-1-{beta}-D-ribofuranoside (AICAR) activation of AMP-activated protein kinase inhibits transepithelial Na+ transport across H441 lung cells J. Physiol., August 1, 2005; 566(3): 781 - 792. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Efendiev, Z. Chen, R. T. Krmar, S. Uhles, A. I. Katz, C. H. Pedemonte, and A. M. Bertorello The 14-3-3 Protein Translates the NA+,K+-ATPase {alpha}1-Subunit Phosphorylation Signal into Binding and Activation of Phosphoinositide 3-Kinase during Endocytosis J. Biol. Chem., April 22, 2005; 280(16): 16272 - 16277. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Efendiev, R. T. Krmar, G. Ogimoto, J. Zwiller, G. Tripodi, A. I. Katz, G. Bianchi, C. H. Pedemonte, and A. M. Bertorello Hypertension-Linked Mutation in the Adducin {alpha}-Subunit Leads to Higher AP2-{micro}2 Phosphorylation and Impaired Na+,K+-ATPase Trafficking in Response to GPCR Signals and Intracellular Sodium Circ. Res., November 26, 2004; 95(11): 1100 - 1108. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Hinojos and P. A. Doris Altered Subcellular Distribution of Na+,K+-ATPase in Proximal Tubules in Young Spontaneously Hypertensive Rats Hypertension, July 1, 2004; 44(1): 95 - 100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Al-Khalili, O. Kotova, H. Tsuchida, I. Ehren, E. Feraille, A. Krook, and A. V. Chibalin ERK1/2 Mediates Insulin Stimulation of Na,K-ATPase by Phosphorylation of the {alpha}-Subunit in Human Skeletal Muscle Cells J. Biol. Chem., June 11, 2004; 279(24): 25211 - 25218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Khundmiri, A. M. Bertorello, N. A. Delamere, and E. D. Lederer Clathrin-mediated Endocytosis of Na+,K+-ATPase in Response to Parathyroid Hormone Requires ERK-dependent Phosphorylation of Ser-11 within the {alpha}1-Subunit J. Biol. Chem., April 23, 2004; 279(17): 17418 - 17427. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Mihailidou, M. Mardini, and J. W. Funder Rapid, Nongenomic Effects of Aldosterone in the Heart Mediated by {epsilon} Protein Kinase C Endocrinology, February 1, 2004; 145(2): 773 - 780. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lei, S. Nowbar, C. N. Mariash, and D. H. Ingbar Thyroid hormone stimulates Na-K-ATPase activity and its plasma membrane insertion in rat alveolar epithelial cells Am J Physiol Lung Cell Mol Physiol, September 1, 2003; 285(3): L762 - L772. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Efendiev, C. E. Budu, A. R. Cinelli, A. M. Bertorello, and C. H. Pedemonte Intracellular Na+ Regulates Dopamine and Angiotensin II Receptors Availability at the Plasma Membrane and Their Cellular Responses in Renal Epithelia J. Biol. Chem., August 1, 2003; 278(31): 28719 - 28726. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. PAGEL, A. ZATTI, T. KIMURA, A. DUFFIELD, V. CHAUVET, V. RAJENDRAN, and M. J. CAPLAN Ion Pump-Interacting Proteins: Promising New Partners Ann. N.Y. Acad. Sci., April 1, 2003; 986(1): 360 - 368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. L. TEIXEIRA, A. I. KATZ, C. H. PEDEMONTE, and A. M. BERTORELLO Isoform-Specific Regulation of Na+,K+-ATPase Endocytosis and Recruitment to the Plasma Membrane Ann. N.Y. Acad. Sci., April 1, 2003; 986(1): 587 - 594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. McDONOUGH, P. K. K. LEONG, and L. E. YANG Mechanisms of Pressure Natriuresis: How Blood Pressure Regulates Renal Sodium Transport Ann. N.Y. Acad. Sci., April 1, 2003; 986(1): 669 - 677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. FERRARI, M. FERRANDI, L. TORIELLI, P. BARASSI, G. TRIPODI, E. MINOTTI, I. MOLINARI, P. MELLONI, and G. BIANCHI Antihypertensive Compounds That Modulate the Na-K Pump Ann. N.Y. Acad. Sci., April 1, 2003; 986(1): 694 - 701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Alzamora, E. T. Marusic, M. Gonzalez, and L. Michea Nongenomic Effect of Aldosterone on Na+,K+-Adenosine Triphosphatase in Arterial Vessels Endocrinology, April 1, 2003; 144(4): 1266 - 1272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Bertorello, Y. Komarova, K. Smith, I. B. Leibiger, R. Efendiev, C. H. Pedemonte, G. Borisy, and J. I. Sznajder Analysis of Na+,K+-ATPase Motion and Incorporation into the Plasma Membrane in Response to G Protein-coupled Receptor Signals in Living Cells Mol. Biol. Cell, March 1, 2003; 14(3): 1149 - 1157. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hussain and M. F. Lokhandwala Renal Dopamine Receptors and Hypertension Experimental Biology and Medicine, February 1, 2003; 228(2): 134 - 142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. A. Narkar, T. Hussain, and M. F. Lokhandwala Activation of D2-like receptors causes recruitment of tyrosine-phosphorylated NKA alpha 1-subunits in kidney Am J Physiol Renal Physiol, December 1, 2002; 283(6): F1290 - F1295. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Efendiev, G. A. Yudowski, J. Zwiller, B. Leibiger, A. I. Katz, P.-O. Berggren, C. H. Pedemonte, I. B. Leibiger, and A. M. Bertorello Relevance of Dopamine Signals Anchoring Dynamin-2 to the Plasma Membrane during Na+,K+-ATPase Endocytosis J. Biol. Chem., November 8, 2002; 277(46): 44108 - 44114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. V. Pierre, M.-J. Duran, D. L. Carr, and T. A. Pressley Structure/function analysis of Na+-K+-ATPase central isoform-specific region: involvement in PKC regulation Am J Physiol Renal Physiol, November 1, 2002; 283(5): F1066 - F1074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Reinhardt, M. Kosch, M. Lerner, H. Bertram, D. Lemke, and H. Oberleithner Stimulation of protein kinase C pathway mediates endocytosis of human nongastric H+-K+-ATPase, ATP1AL1 Am J Physiol Renal Physiol, August 1, 2002; 283(2): F335 - F343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Done, I. B. Leibiger, R. Efendiev, A. I. Katz, B. Leibiger, P.-O. Berggren, C. H. Pedemonte, and A. M. Bertorello Tyrosine 537 within the Na+,K+-ATPase alpha -Subunit Is Essential for AP-2 Binding and Clathrin-dependent Endocytosis J. Biol. Chem., May 3, 2002; 277(19): 17108 - 17111. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Efendiev, A. M. Bertorello, R. Zandomeni, A. R. Cinelli, and C. H. Pedemonte Agonist-dependent Regulation of Renal Na+,K+-ATPase Activity Is Modulated by Intracellular Sodium Concentration J. Biol. Chem., March 22, 2002; 277(13): 11489 - 11496. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Khundmiri and E. Lederer PTH and DA regulate Na-K ATPase through divergent pathways Am J Physiol Renal Physiol, March 1, 2002; 282(3): F512 - F522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Sweeney, W. Niu, V. A. Canfield, R. Levenson, and A. Klip Insulin increases plasma membrane content and reduces phosphorylation of Na+-K+ pump alpha 1-subunit in HEK-293 cells Am J Physiol Cell Physiol, December 1, 2001; 281(6): C1797 - C1803. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. MAGYAR, Y. ZHANG, N.-H. HOLSTEIN-RATHLOU, and A. A. MCDONOUGH Downstream Shift in Sodium Pump Activity along the Nephron during Acute Hypertension J. Am. Soc. Nephrol., November 1, 2001; 12(11): 2231 - 2240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. DJELIDI, A. BEGGAH, N. COURTOIS-COUTRY, M. FAY, F. CLUZEAUD, S. VIENGCHAREUN, J.-P. BONVALET, N. FARMAN, and M. BLOT-CHABAUD Basolateral Translocation by Vasopressin of the Aldosterone-Induced Pool of Latent Na-K-ATPases Is Accompanied by {alpha}1 Subunit Dephosphorylation: Study in a New Aldosterone-Sensitive Rat Cortical Collecting Duct Cell Line J. Am. Soc. Nephrol., September 1, 2001; 12(9): 1805 - 1818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Carey Renal Dopamine System: Paracrine Regulator of Sodium Homeostasis and Blood Pressure Hypertension, September 1, 2001; 38(3): 297 - 302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. ASGHAR, V. KANSRA, T. HUSSAIN, and M. F. LOKHANDWALA Hyperphosphorylation of Na-Pump Contributes to Defective Renal Dopamine Response in Old Rats J. Am. Soc. Nephrol., February 1, 2001; 12(2): 226 - 232. [Abstract] [Full Text]
|
|
|
|
|
|
S. Gonin, G. Deschênes, F. Roger, M. Bens, P.-Y. Martin, J.-L. Carpentier, A. Vandewalle, A. Doucet, and E. Féraille Cyclic AMP Increases Cell Surface Expression of Functional Na,K-ATPase Units in Mammalian Cortical Collecting Duct Principal Cells Mol. Biol. Cell, February 1, 2001; 12(2): 255 - 264. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. Feraille and A. Doucet Sodium-Potassium-Adenosinetriphosphatase-Dependent Sodium Transport in the Kidney: Hormonal Control Physiol Rev, January 1, 2001; 81(1): 345 - 418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nowicki, M. S. Kruse, H. Brismar, and A. Aperia Dopamine-induced translocation of protein kinase C isoforms visualized in renal epithelial cells Am J Physiol Cell Physiol, December 1, 2000; 279(6): C1812 - C1818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. Therien and R. Blostein Mechanisms of sodium pump regulation Am J Physiol Cell Physiol, September 1, 2000; 279(3): C541 - C566. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Féraille, P. Béguin, M.-L. Carranza, S. Gonin, M. Rousselot, P.-Y. Martin, H. Favre, and K. Geering Is Phosphorylation of the alpha 1 Subunit at Ser-16 Involved in the Control of Na,K-ATPase Activity by Phorbol Ester-activated Protein Kinase C? Mol. Biol. Cell, January 1, 2000; 11(1): 39 - 50. [Abstract] [Full Text]
|
|
|
|
|
|
M. S. Feschenko, E. Stevenson, and K. J. Sweadner Interaction of Protein Kinase C and cAMP-dependent Pathways in the Phosphorylation of the Na,K-ATPase J. Biol. Chem., October 27, 2000; 275(44): 34693 - 34700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Dunbar and M. J. Caplan Ion Pumps in Polarized Cells: Sorting and Regulation of the Na+,K+- and H+,K+-ATPases J. Biol. Chem., August 3, 2001; 276(32): 29617 - 29620. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ogimoto, G. A. Yudowski, C. J. Barker, M. Kohler, A. I. Katz, E. Feraille, C. H. Pedemonte, P.-O. Berggren, and A. M. Bertorello G protein-coupled receptors regulate Na+,K+-ATPase activity and endocytosis by modulating the recruitment of adaptor protein 2 and clathrin PNAS, March 28, 2000; 97(7): 3242 - 3247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Yudowski, R. Efendiev, C. H. Pedemonte, A. I. Katz, P.-O. Berggren, and A. M. Bertorello Phosphoinositide-3 kinase binds to a proline-rich motif in the Na+,K+-ATPase alpha subunit and regulates its trafficking PNAS, June 6, 2000; 97(12): 6556 - 6561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Yang, P. K. K. Leong, J. O. Chen, N. Patel, S. F. Hamm-Alvarez, and A. A. McDonough Acute hypertension provokes internalization of proximal tubule NHE3 without inhibition of transport activity Am J Physiol Renal Physiol, April 1, 2002; 282(4): F730 - F740. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Gomes and P. Soares-da-Silva Role of cAMP-PKA-PLC signaling cascade on dopamine-induced PKC-mediated inhibition of renal Na+-K+-ATPase activity Am J Physiol Renal Physiol, June 1, 2002; 282(6): F1084 - F1096. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Ridge, L. Dada, E. Lecuona, A. M. Bertorello, A. I. Katz, D. Mochly-Rosen, and J. I. Sznajder Dopamine-induced Exocytosis of Na,K-ATPase Is Dependent on Activation of Protein Kinase C-epsilon and -delta Mol. Biol. Cell, April 1, 2002; 13(4): 1381 - 1389. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
