| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 4, 1957-1965, January 22, 1999
Subunit
in Human Embryonal Kidney 293 Cells
From the Faculty of Bioscience and Biotechnology, Tokyo Institute
of Technology, 4259 Nagatsuta-cho, Midori-ku,
Yokohama 226-8501, Japan
Heterotrimeric G protein 
subunit (G)
mediates signals to two types of stress-activated protein kinases,
c-Jun NH2-terminal kinase (JNK) and p38
mitogen-activated protein kinase, in mammalian cells. To investigate
the signaling mechanism whereby G regulates the activity of JNK,
we transfected kinase-deficient mutants of two JNK kinases,
mitogen-activated protein kinase kinase 4 (MKK4) and 7 (MKK7), into
human embryonal kidney 293 cells. G-induced JNK activation was
blocked by kinase-deficient MKK4 and to a lesser extent by
kinase-deficient MKK7. Moreover, G increased MKK4 activity by
6-fold and MKK7 activity by 2-fold. MKK4 activation by G was
blocked by dominant-negative Rho and Cdc42, whereas MKK7 activation was
blocked by dominant-negative Rac. In addition, G-mediated MKK4
activation, but not MKK7 activation, was inhibited completely by
specific tyrosine kinase inhibitors PP2 and PP1. These results indicate
that G induces JNK activation mainly through MKK4 activation
dependent on Rho, Cdc42, and tyrosine kinase, and to a lesser extent
through MKK7 activation dependent on Rac.
This article has been cited by other articles:
|
|
 |
|
  B. Liu, J.-f. Wu, Y.-y. Zhan, H.-z. Chen, X.-y. Zhang, and Q. Wu Regulation of the Orphan Receptor TR3 Nuclear Functions by c-Jun N Terminal Kinase Phosphorylation Endocrinology, January 1, 2007; 148(1): 34 - 44. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Miyamoto, J. Yamauchi, N. Mizuno, and H. Itoh The Adaptor Protein Nck1 Mediates Endothelin A Receptor-regulated Cell Migration through the Cdc42-dependent c-Jun N-terminal Kinase Pathway J. Biol. Chem., August 13, 2004; 279(33): 34336 - 34342. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Y. F. Kam, A. S. L. Chan, and Y. H. Wong {kappa}-Opioid Receptor Signals through Src and Focal Adhesion Kinase to Stimulate c-Jun N-Terminal Kinases in Transfected COS-7 Cells and Human Monocytic THP-1 Cells J. Pharmacol. Exp. Ther., July 1, 2004; 310(1): 301 - 310. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Inoue, T. Zama, T. Kamimoto, R. Aoki, Y. Ikeda, H. Kimura, and M. Hagiwara TNF{alpha}-induced ATF3 expression is bidirectionally regulated by the JNK and ERK pathways in vascular endothelial cells Genes Cells, January 1, 2004; 9(1): 59 - 70. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Uma, P. Muraly, S. Verma-Kumar, and R. Medhamurthy Determination of Onset of Apoptosis in Granulosa Cells of the Preovulatory Follicles in the Bonnet Monkey (Macaca radiata): Correlation with Mitogen-Activated Protein Kinase Activities Biol Reprod, October 1, 2003; 69(4): 1379 - 1387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Miyamoto, J. Yamauchi, and H. Itoh Src Kinase Regulates the Activation of a Novel FGD-1-related Cdc42 Guanine Nucleotide Exchange Factor in the Signaling Pathway from the Endothelin A Receptor to JNK J. Biol. Chem., August 8, 2003; 278(32): 29890 - 29900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Seo, Y.-I. Lee, C.-H. Cho, C.-D. Bae, I.-H. Kim, and Y.-S. Juhnn Bi-directional Regulation of UV-induced Activation of p38 Kinase and c-Jun N-terminal Kinase by G Protein beta gamma -Subunits J. Biol. Chem., June 28, 2002; 277(27): 24197 - 24203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Parnell, B. S. Magenheimer, R. L. Maser, C. A. Zien, A.-M. Frischauf, and J. P. Calvet Polycystin-1 Activation of c-Jun N-terminal Kinase and AP-1 Is Mediated by Heterotrimeric G Proteins J. Biol. Chem., May 24, 2002; 277(22): 19566 - 19572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ueda, R. Morishita, H. Itoh, S. Narumiya, K. Mikoshiba, K. Kato, and T. Asano Galpha 11 Induces Caspase-mediated Proteolytic Activation of Rho-associated Kinase, ROCK-I, in HeLa Cells J. Biol. Chem., November 2, 2001; 276(45): 42527 - 42533. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Xu, A. C. Maroney, P. Dobrzanski, N. V. Kukekov, and L. A. Greene The MLK Family Mediates c-Jun N-Terminal Kinase Activation in Neuronal Apoptosis Mol. Cell. Biol., July 15, 2001; 21(14): 4713 - 4724. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Pearson, F. Robinson, T. Beers Gibson, B.-e Xu, M. Karandikar, K. Berman, and M. H. Cobb Mitogen-Activated Protein (MAP) Kinase Pathways: Regulation and Physiological Functions Endocr. Rev., April 1, 2001; 22(2): 153 - 183. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Chayama, P. J. Papst, T. P. Garrington, J. C. Pratt, T. Ishizuka, S. Webb, S. Ganiatsas, L. I. Zon, W. Sun, G. L. Johnson, et al. Role of MEKK2-MEK5 in the regulation of TNF-alpha gene expression and MEKK2-MKK7 in the activation of c-Jun N-terminal kinase in mast cells PNAS, March 22, 2001; (2001) 81021898. [Abstract] [Full Text]
|
|
|
|
|
|
A. S. L. Chan and Y. H. Wong Regulation of c-Jun N-Terminal Kinase by the ORL1 Receptor through Multiple G Proteins J. Pharmacol. Exp. Ther., December 1, 2000; 295(3): 1094 - 1100. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. Rueda, I. Galve-Roperh, A. Haro, and M. Guzmán The CB1 Cannabinoid Receptor Is Coupled to the Activation of c-Jun N-Terminal Kinase Mol. Pharmacol., October 1, 2000; 58(4): 814 - 820. [Abstract] [Full Text]
|
|
|
|
|
|
A. Blaukat, A. Barac, M. J. Cross, S. Offermanns, and I. Dikic G Protein-Coupled Receptor-Mediated Mitogen-Activated Protein Kinase Activation through Cooperation of Galpha q and Galpha i Signals Mol. Cell. Biol., September 15, 2000; 20(18): 6837 - 6848. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. Cai, C. Zhang, T. Nawa, T. Aso, M. Tanaka, S. Oshiro, H. Ichijo, and S. Kitajima Homocysteine-responsive ATF3 gene expression in human vascular endothelial cells: activation of c-Jun NH2-terminal kinase and promoter response element Blood, September 15, 2000; 96(6): 2140 - 2148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yoshida, R. Weichselbaum, S. Kharbanda, and D. Kufe Role for Lyn Tyrosine Kinase as a Regulator of Stress-Activated Protein Kinase Activity in Response to DNA Damage Mol. Cell. Biol., August 1, 2000; 20(15): 5370 - 5380. [Abstract] [Full Text]
|
|
|
|
|
|
J. Yamauchi, T. Kawano, M. Nagao, Y. Kaziro, and H. Itoh Gi-dependent Activation of c-Jun N-terminal Kinase in Human Embryonal Kidney 293 Cells J. Biol. Chem., March 10, 2000; 275(11): 7633 - 7640. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ueda, H. Itoh, J. Yamauchi, R. Morishita, Y. Kaziro, K. Kato, and T. Asano G Protein beta gamma Subunits Induce Stress Fiber Formation and Focal Adhesion Assembly in a Rho-dependent Manner in HeLa Cells J. Biol. Chem., January 21, 2000; 275(3): 2098 - 2102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Meza, R. Bannister, K. Melliti, and B. Adams Biphasic, Opposing Modulation of Cloned Neuronal alpha 1E Ca Channels by Distinct Signaling Pathways Coupled to M2 Muscarinic Acetylcholine Receptors J. Neurosci., August 15, 1999; 19(16): 6806 - 6817. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nakano, J. Yamauchi, K. Nakagawa, H. Itoh, and N. Kitamura NESK, a Member of the Germinal Center Kinase Family That Activates the c-Jun N-terminal Kinase Pathway and Is Expressed during the Late Stages of Embryogenesis J. Biol. Chem., June 30, 2000; 275(27): 20533 - 20539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Sellers, J. Simon, T. S. Lundahl, D. J. Cousens, P. P. A. Humphrey, and E. A. Barnard Adenosine Nucleotides Acting at the Human P2Y1 Receptor Stimulate Mitogen-activated Protein Kinases and Induce Apoptosis J. Biol. Chem., May 4, 2001; 276(19): 16379 - 16390. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Huai and U. Drescher An ephrin-A-dependent Signaling Pathway Controls Integrin Function and Is Linked to the Tyrosine Phosphorylation of a 120-kDa Protein J. Biol. Chem., February 23, 2001; 276(9): 6689 - 6694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ludwig, C. Ehrhardt, E. R. Neumeier, M. Kracht, U. R. Rapp, and S. Pleschka Influenza Virus-induced AP-1-dependent Gene Expression Requires Activation of the JNK Signaling Pathway J. Biol. Chem., March 30, 2001; 276(14): 10990 - 10998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yamauchi, G. Tsujimoto, Y. Kaziro, and H. Itoh Parallel Regulation of Mitogen-activated Protein Kinase Kinase 3 (MKK3) and MKK6 in Gq-signaling Cascade J. Biol. Chem., June 22, 2001; 276(26): 23362 - 23372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Wada, K. Nakagawa, T. Watanabe, G. Nishitai, J. Seo, H. Kishimoto, D. Kitagawa, T. Sasaki, J. M. Penninger, H. Nishina, et al. Impaired Synergistic Activation of Stress-activated Protein Kinase SAPK/JNK in Mouse Embryonic Stem Cells Lacking SEK1/MKK4. DIFFERENT CONTRIBUTION OF SEK2/MKK7 ISOFORMS TO THE SYNERGISTIC ACTIVATION J. Biol. Chem., August 10, 2001; 276(33): 30892 - 30897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Chayama, P. J. Papst, T. P. Garrington, J. C. Pratt, T. Ishizuka, S. Webb, S. Ganiatsas, L. I. Zon, W. Sun, G. L. Johnson, et al. Role of MEKK2-MEK5 in the regulation of TNF-alpha gene expression and MEKK2-MKK7 in the activation of c-Jun N-terminal kinase in mast cells PNAS, April 10, 2001; 98(8): 4599 - 4604. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
