| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 4, 2097-2106, January 22, 1999
From the Department of Pathology, University of Colorado Health
Sciences Center, Denver, Colorado 80262
We have investigated the interaction
between Cbl and the Src-related tyrosine kinase Fyn. Fyn was observed
to be constitutively associated with Cbl in lysates of several
different cell types including the interleukin-3-dependent
murine myeloid cell line 32Dcl3, and the prolactin-dependent
rat thymoma cell line Nb2. Binding studies indicated that Cbl could
bind to glutathione S-transferase (GST) fusion proteins
encoding the unique, Src homology domain 3 (SH3), and SH2 domains of
Fyn, Hck, or Lyn. Fusion proteins encoding either the SH3 or SH2
domains of Fyn bound to Cbl as effectively as the fusion protein
encoding the unique, SH3, and SH2 domains of Fyn. The Fyn SH2 domain
bound to both tyrosine-phosphorylated and nonphosphorylated Cbl,
implying that this interaction might be phosphotyrosine-independent.
Binding of the Fyn SH2 domain to Cbl was not disrupted by the addition
of phosphotyrosine, phosphoserine, or phosphothreonine. A GST fusion
protein encoding the proline-rich region of Cbl bound to Fyn present in
a total cell lysate. Far Western blot analysis also indicated that the
SH3 domain of Fyn bound preferentially to the proline-rich region of
Cbl. The addition of [
-32P]ATP to either anti-Cbl
immunoprecipitates or anti-Fyn immunoprecipitates resulted in the
phosphorylation of both Cbl and Fyn as demonstrated by
immunoprecipitation of the phosphorylated proteins with specific antisera. Fyn directly phosphorylated a GST fusion protein containing the C-terminal region of Cbl (GST-CBL-LZIP). In contrast,
immunoprecipitated JAK2 was not able to phosphorylate this same region
of Cbl. The GST-CBL-LZIP fusion protein contains a binding site for the
SH2 domain of the p85 subunit of phosphatidylinositol 3-kinase, which mapped to Tyr731, which is present in the sequence YEAM.
Mutation of Tyr731 in GST-CBL-LZIP eliminated binding of
the p85 subunit of phosphatidylinositol 3-kinase and substantially
reduced the phosphorylation of this fusion protein by Fyn, despite the
presence of four other tyrosine residues in this fusion protein. These
data are consistent with the hypothesis that Cbl represents a substrate
for Src-like kinases that are activated in response to the engagement
of cell surface receptors, and that Src-like kinases are responsible
for the phosphorylation of a tyrosine residue in Cbl that may regulate
activation of phosphatidylinositol 3-kinase.
This article has been cited by other articles:
|
|
 |
|
  M. B. Einarson, E. N. Pugacheva, and J. R. Orlinick Identification of Protein-Protein Interactions with Glutathione-S-Transferase (GST) Fusion Proteins CSH Protocols, July 1, 2007; 2007(16): pdb.top11 - pdb.top11. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. MANKAI, J.-R. EVEILLARD, V. BUHE, K. LE STER, S. LOISEL, I. GHEDIRA, P. YOUINOU, C. BERTHOU, and A. BORDRON Is the c-Cbl Proto-Oncogene Involved in Chronic Lymphocytic Leukemia? Ann. N.Y. Acad. Sci., June 1, 2007; 1107(1): 193 - 205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Molero, N. Turner, C. B.F. Thien, W. Y. Langdon, D. E. James, and G. J. Cooney Genetic Ablation of the c-Cbl Ubiquitin Ligase Domain Results in Increased Energy Expenditure and Improved Insulin Action Diabetes, December 1, 2006; 55(12): 3411 - 3417. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Gustin, C. B. F. Thien, and W. Y. Langdon Cbl-b Is a Negative Regulator of Inflammatory Cytokines Produced by IgE-Activated Mast Cells J. Immunol., November 1, 2006; 177(9): 5980 - 5989. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Koga, W. Xu, T. S. Karpova, J. G. McNally, R. Baron, and L. Neckers Hsp90 inhibition transiently activates Src kinase and promotes Src-dependent Akt and Erk activation PNAS, July 25, 2006; 103(30): 11318 - 11322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lei, C. N. Mariash, and D. H. Ingbar 3,3',5-Triiodo-L-thyronine Up-regulation of Na,K-ATPase Activity and Cell Surface Expression in Alveolar Epithelial Cells Is Src Kinase- and Phosphoinositide 3-Kinase-dependent J. Biol. Chem., November 12, 2004; 279(46): 47589 - 47600. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kaabeche, J. Lemonnier, S. Le Mee, J. Caverzasio, and P. J. Marie Cbl-mediated Degradation of Lyn and Fyn Induced by Constitutive Fibroblast Growth Factor Receptor-2 Activation Supports Osteoblast Differentiation J. Biol. Chem., August 27, 2004; 279(35): 36259 - 36267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Ghosh, A. L. Reddi, N. L. Rao, L. Duan, V. Band, and H. Band Biochemical Basis for the Requirement of Kinase Activity for Cbl-dependent Ubiquitinylation and Degradation of a Target Tyrosine Kinase J. Biol. Chem., August 20, 2004; 279(34): 36132 - 36141. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. O. Watson and W. T. Lee Differences in Signaling Molecule Organization between Naive and Memory CD4+ T Lymphocytes J. Immunol., July 1, 2004; 173(1): 33 - 41. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Haglund, I. Ivankovic-Dikic, N. Shimokawa, G. D. Kruh, and I. Dikic Recruitment of Pyk2 and Cbl to lipid rafts mediates signals important for actin reorganization in growing neurites J. Cell Sci., May 15, 2004; 117(12): 2557 - 2568. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Bailey, K. M. Nieport, M. P. Herbst, S. Srivastava, R. A. Serra, and N. D. Horseman Prolactin and Transforming Growth Factor-{beta} Signaling Exert Opposing Effects on Mammary Gland Morphogenesis, Involution, and the Akt-Forkhead Pathway Mol. Endocrinol., May 1, 2004; 18(5): 1171 - 1184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Miyazaki, A. Sanjay, L. Neff, S. Tanaka, W. C. Horne, and R. Baron Src Kinase Activity Is Essential for Osteoclast Function J. Biol. Chem., April 23, 2004; 279(17): 17660 - 17666. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. I. Taher, E. P. M. Tjin, E. A. Beuling, J. Borst, M. Spaargaren, and S. T. Pals c-Cbl Is Involved in Met Signaling in B Cells and Mediates Hepatocyte Growth Factor-Induced Receptor Ubiquitination J. Immunol., October 1, 2002; 169(7): 3793 - 3800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. L. K. Goh, T. Zhu, W.-Y. Leong, and P. E. Lobie c-Cbl Is a Negative Regulator of GH-Stimulated STAT5-Mediated Transcription Endocrinology, September 1, 2002; 143(9): 3590 - 3603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. K. Kassenbrock, S. Hunter, P. Garl, G. L. Johnson, and S. M. Anderson Inhibition of Src Family Kinases Blocks Epidermal Growth Factor (EGF)-induced Activation of Akt, Phosphorylation of c-Cbl, and Ubiquitination of the EGF Receptor J. Biol. Chem., July 5, 2002; 277(28): 24967 - 24975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Tang, S. K. Subudhi, K. J. Henriksen, C. G. Long, F. Vives, and J. A. Bluestone The Src Family Kinase Fyn Mediates Signals Induced by TCR Antagonists J. Immunol., May 1, 2002; 168(9): 4480 - 4487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Frasor, U. Barkai, L. Zhong, A. T. Fazleabas, and G. Gibori PRL-Induced ER{alpha} Gene Expression Is Mediated by Janus Kinase 2 (Jak2) While Signal Transducer and Activator of Transcription 5b (Stat5b) Phosphorylation Involves Jak2 and a Second Tyrosine Kinase Mol. Endocrinol., November 1, 2001; 15(11): 1941 - 1952. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C V Clevenger and J B Kline Prolactin receptor signal transduction Lupus, October 1, 2001; 10(10): 706 - 718. [Abstract] [PDF]
|
|
|
|
 |
|
 H. Gu, H. Maeda, J. J. Moon, J. D. Lord, M. Yoakim, B. H. Nelson, and B. G. Neel New Role for Shc in Activation of the Phosphatidylinositol 3-Kinase/Akt Pathway Mol. Cell. Biol., October 1, 2000; 20(19): 7109 - 7120. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. M. Fournier, L. Lamorte, C. R. Maroun, M. Lupher, H. Band, W. Langdon, and M. Park Cbl-transforming Variants Trigger a Cascade of Molecular Alterations That Lead to Epithelial Mesenchymal Conversion Mol. Biol. Cell, October 1, 2000; 11(10): 3397 - 3410. [Abstract] [Full Text]
|
|
|
|
|
|
G. Scholz, K. Cartledge, and A. R. Dunn Hck Enhances the Adherence of Lipopolysaccharide-stimulated Macrophages via Cbl and Phosphatidylinositol 3-Kinase J. Biol. Chem., May 5, 2000; 275(19): 14615 - 14623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Sato, M.-K. Kim, and A. D. Schreiber Enhancement of Fc{gamma} Receptor-Mediated Phagocytosis by Transforming Mutants of Cbl J. Immunol., December 1, 1999; 163(11): 6123 - 6131. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. K. Goh, T. Zhu, S. Yakar, D. LeRoith, and P. E. Lobie CrkII Participation in the Cellular Effects of Growth Hormone and Insulin-like Growth Factor-1. PHOSPHATIDYLINOSITOL-3 KINASE DEPENDENT AND INDEPENDENT EFFECTS J. Biol. Chem., June 2, 2000; 275(23): 17683 - 17692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Hamilton, K. M. Miller, K. M. Helm, W. Y. Langdon, and S. M. Anderson Suppression of Apoptosis Induced by Growth Factor Withdrawal by an Oncogenic Form of c-Cbl J. Biol. Chem., March 16, 2001; 276(12): 9028 - 9037. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Arron, M. Vologodskaia, B. R. Wong, M. Naramura, N. Kim, H. Gu, and Y. Choi A Positive Regulatory Role for Cbl Family Proteins in Tumor Necrosis Factor-related Activation-induced Cytokine (TRANCE) and CD40L-mediated Akt Activation J. Biol. Chem., August 3, 2001; 276(32): 30011 - 30017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gelkop, Y. Babichev, and N. Isakov T Cell Activation Induces Direct Binding of the Crk Adapter Protein to the Regulatory Subunit of Phosphatidylinositol 3-Kinase (p85) via a Complex Mechanism Involving the Cbl Protein J. Biol. Chem., September 21, 2001; 276(39): 36174 - 36182. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
