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J Biol Chem, Vol. 274, Issue 4, 2107-2112, January 22, 1999

Multiple Pathways of Apoptosis in PC12 Cells
CrmA INHIBITS APOPTOSIS INDUCED BY -AMYLOID

Kathryn J. Ivins, Jonathan K. Ivins^¶, Jason P. Sharp, and Carl W. Cotman

From the  Institute for Brain Aging and Dementia and the ^¶ Department of Developmental and Cell Biology, University of California at Irvine, Irvine, California 92697

Stable transfectants of PC12 cells expressing bcl-2 or crmA were generated and tested for their susceptibility to various apoptotic insults. Bcl-2 expression conferred resistance to apoptosis induced by staurosporine and by oxidative insults including hydrogen peroxide and peroxynitrite, but was less effective in inhibition of activation-induced programmed cell death induced by concanavalin A. Concanavalin A-induced apoptosis was abated, however, in cells expressing very high levels of bcl-2. In contrast, cells expressing crmA were protected from concanavalin A-induced apoptosis, but were as susceptible as control cells to apoptosis induced by staurosporine and oxidative insults. Therefore, at least two apoptotic pathways in PC12 cells can be discerned by their differential sensitivity to blockade by bcl-2 and crmA. The ability of -amyloid (A) to induce apoptosis in these cells was assessed. CrmA transfectants were protected from apoptosis induced by A_1-42, but only cells expressing very high levels of bcl-2 were similarly protected. These results suggest that the apoptotic pathway activated by A_1-42 in PC12 cells can be differentiated from the apoptotic pathway activated by oxidative insults. Gene transfer experiments also demonstrated that expression of crmA in primary cultures of hippocampal neurons is protective against cell death induced by A_1-42. Together these results support the hypothesis that A-induced apoptosis occurs through activation-induced programmed cell death.

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Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.

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