HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Takada, Y. Articles by Akashi, M. Search for Related Content PubMed PubMed Citation Articles by Takada, Y. Articles by Akashi, M. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 40, 28286-28292, October 1, 1999

12-O-tetradecanoylphorbol-13-acetate-induced Apoptosis Is Mediated by Tumor Necrosis Factor  in Human Monocytic U937 Cells

Yasunari Takada, Misao Hachiya, Yoshiaki Osawa, Yoshinori Hasegawa^§, Koichi Ando^¶, Yoshiro Kobayashi^**, and Makoto Akashi

From the  Division of Radiation Health, ^¶ Space and Particle Radiation Science Research Group, National Institute of Radiological Sciences, Chiba, 263-8555 Japan, ^§ First Department of Internal Medicine, Nagoya University School of Medicine, Nagoya 466-8550, Japan, and ^** Laboratory of Molecular Immunology, Department of Biomolecular Science, Faculty of Science, Toho University, Funabashi 274-8510, Japan

12-O-tetradecanoylphorbol-13-acetate (TPA), a phorbol ester that is known as a tumor promoter, induces differentiation of myeloid cells and suppresses their proliferation. We studied the regulation of apoptosis by TPA in human monocytic cell line U937 cells that lack p53. Untreated U937 cells constitutively underwent apoptosis, and TPA enhanced apoptosis in these cells. Further studies showed that TPA increased production of tumor necrosis factor- (TNF) in U937 cells, and exogenously added TNF induced apoptosis. Moreover, the induction of apoptosis by TPA was blocked by anti-TNF antibody. Similar results were obtained in the myeloblastic cell line KY821 cells. We also found that the induction of apoptosis by TPA was increased in cells overexpressed with TNF receptor 1 but not in control cells. Furthermore, TPA failed to induce the production of TNF and apoptosis in cells with either their protein kinase C or mitogen-activated protein kinase pathway blocked. Our results indicate that TPA induces apoptosis, at least in part, through a pathway that requires endogenous production of TNF in U937 cells. Our data also suggest that the induction of apoptosis by TPA occurs through activation of protein kinase C and mitogen-activated protein kinase and TNF is an autocrine-stimulating factor for the induction of apoptosis in these cells.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				B. A. Hug, N. Ahmed, J. A. Robbins, and M. A. Lazar A Chromatin Immunoprecipitation Screen Reveals Protein Kinase C{beta} as a Direct RUNX1 Target Gene J. Biol. Chem., January 9, 2004; 279(2): 825 - 830. [Abstract] [Full Text] [PDF]

				J.-M. Lai, C.-L. Hsieh, and Z.-F. Chang Caspase activation during phorbol ester-induced apoptosis requires ROCK-dependent myosin-mediated contraction J. Cell Sci., September 1, 2003; 116(17): 3491 - 3501. [Abstract] [Full Text] [PDF]

				L. Cartee, S. C. Maggio, R. Smith, H. M. Sankala, P. Dent, and S. Grant Protein Kinase C-dependent Activation of the Tumor Necrosis Factor Receptor-mediated Extrinsic Cell Death Pathway Underlies Enhanced Apoptosis in Human Myeloid Leukemia Cells Exposed to Bryostatin 1 and Flavopiridol Mol. Cancer Ther., January 1, 2003; 2(1): 83 - 93. [Abstract] [Full Text] [PDF]

				S. Wang, Z. Wang, and S. Grant Bryostatin 1 and UCN-01 Potentiate 1-beta -D-Arabinofuranosylcytosine-Induced Apoptosis in Human Myeloid Leukemia Cells through Disparate Mechanisms Mol. Pharmacol., January 1, 2003; 63(1): 232 - 242. [Abstract] [Full Text] [PDF]

				O. Sordet, C. Rebe, S. Plenchette, Y. Zermati, O. Hermine, W. Vainchenker, C. Garrido, E. Solary, and L. Dubrez-Daloz Specific involvement of caspases in the differentiation of monocytes into macrophages Blood, December 15, 2002; 100(13): 4446 - 4453. [Abstract] [Full Text] [PDF]

				J.-M. Lai, S. Wu, D.-Y. Huang, and Z.-F. Chang Cytosolic Retention of Phosphorylated Extracellular Signal-Regulated Kinase and a Rho-Associated Kinase-Mediated Signal Impair Expression of p21Cip1/Waf1 in Phorbol 12-Myristate-13- Acetate-Induced Apoptotic Cells Mol. Cell. Biol., November 1, 2002; 22(21): 7581 - 7592. [Abstract] [Full Text] [PDF]

				L. Cartee, R. Smith, Y. Dai, M. Rahmani, R. Rosato, J. Almenara, P. Dent, and S. Grant Synergistic Induction of Apoptosis in Human Myeloid Leukemia Cells by Phorbol 12-Myristate 13-Acetate and Flavopiridol Proceeds via Activation of Both the Intrinsic and Tumor Necrosis Factor-Mediated Extrinsic Cell Death Pathways Mol. Pharmacol., June 1, 2002; 61(6): 1313 - 1321. [Abstract] [Full Text] [PDF]

				X. Hu, W. E. Janssen, L. C. Moscinski, M. Bryington, A. Dangsupa, N. Rezai-Zadeh, B. A. Babbin, and K. S. Zuckerman An I{kappa}B{alpha} Inhibitor Causes Leukemia Cell Death through a p38 MAP Kinase-dependent, NF-{kappa}B-independent Mechanism Cancer Res., August 1, 2001; 61(16): 6290 - 6296. [Abstract] [Full Text] [PDF]