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J Biol Chem, Vol. 274, Issue 40, 28652-28659, October 1, 1999

Relationship between Phosphatidic Acid Level and Regulation of Protein Transit in Colonic Epithelial Cell Line HT29-cl19A

From the Laboratoire de Biochimie des Transports Cellulaires, CNRS, Unité Mixte de Recherche 8619, Bâtiment 432, Université Paris XI, 91 405 Orsay Cedex, France

Colonic epithelial HT29-cl19A cells are polarized and secrete proteins among which ₁-antitrypsin represents about 95%. Secretion occurs via a constitutive pathway, so that the rates of secretion directly reflect the rates of protein transit. In this paper we have demonstrated that: 1) in resting cells phospholipase D (PLD) is implicated in the control of apical protein transit; 2) phorbol esters stimulate apical protein transit (stimulation factor 2.2), which is correlated with a PLD-catalyzed production of phosphatidic acid (PA) (2.45-fold increase); 3) the stimulation of cholinergic receptors by carbachol results in an increase (stimulation factor 1.45) of apical protein transit which is independent of protein kinase C and PLD activities, but related to PA formation (1.7-fold increase) via phospholipase(s) C and diacylglycerol kinase activation; 4) an elevation of the cAMP level enhances apical protein transit by a PA-independent mechanism; 5) a trans-Golgi network or post-trans-Golgi network step of the transit is the target for the regulatory events. In conclusion, we have shown that PA can be produced by two independent signaling pathways; whatever the pathway followed, a close relationship between the amount of PA and the level of secretion was observed.

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