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J Biol Chem, Vol. 274, Issue 42, 29683-29688, October 15, 1999
From the Department of Biochemistry, Wake Forest University School
of Medicine, Winston-Salem, North Carolina 27157
In addition to the CDP-choline pathway for
phosphatidylcholine (PC) synthesis, the liver has a unique
phosphatidylethanolamine (PE) methyltransferase activity for PC
synthesis via three methylations of the ethanolamine moiety of PE.
Previous studies indicate that the two pathways are functionally
different and not interchangeable even though PC is the common product
of both pathways. This study was designed to test the hypothesis that
these two pathways produce different profiles of PC species. The PC
species from these two pathways were labeled with specific stable
isotope precursors, D9-choline and D4-ethanolamine, and analyzed by
electrospray tandem mass spectrometry. Our studies revealed a profound
distinction in PC profiles between the CDP-choline pathway and the PE
methylation pathway. PC molecules produced from the CDP-choline pathway
were mainly comprised of medium chain, saturated (e.g.
16:0/18:0) species. On the other hand, PC molecules from the PE
methylation pathway were much more diverse and were comprised of
significantly more long chain, polyunsaturated (e.g.
18:0/20:4) species. PC species from the methylation pathway contained a
higher percentage of arachidonate and were more diverse than those from
the CDP-choline pathway. This profound distinction of PC profiles may
contribute to the different functions of these two pathways in the liver.
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