| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 43, 30345-30348, October 22, 1999
From the Departments of Cell Biology and Physiology and Medicine,
University of Pittsburgh School of Medicine,
Pittsburgh, Pennsylvania 15261
The first step in transepithelial sodium
absorption lies at the apical membrane where the amiloride-sensitive,
epithelial sodium channel, ENaC, facilitates sodium entry into the
cell. Here we report that the vesicle traffic regulatory (SNARE
(soluble N-ethylmaleimide-sensitive factor attachment
protein receptor)) protein, syntaxin 1A (S1A), inhibits ENaC mediated
sodium entry. This inhibitory effect is selective for S1A and is not
reproduced by syntaxin 3. The inhibition does not require the membrane
anchoring domain of syntaxin 1A. It was reversed by the S1A-binding
protein, Munc-18, but not by a Munc-18 mutant, which lacks syntaxin
affinity. Immunostaining of epitope-tagged ENaC subunits showed that
syntaxin 1A decreases ENaC current by reducing the number of ENaC
channels in the plasma membrane; S1A does not interfere with ENaC
protein expression. Immunoprecipitation of syntaxin 1A from the
sodium-transporting epithelial cell line, A6, co-precipitates ENaC.
These findings indicate that syntaxin 1A and other members of the SNARE
machinery are involved in the control of plasma membrane ENaC content,
and they suggest that SNARE proteins participate in the regulation of
sodium absorption in relation to agonist mediated vesicle
insertion-retrieval processes.
This article has been cited by other articles:
|
|
 |
|
  W. G. Hill, M. B. Butterworth, H. Wang, R. S. Edinger, J. Lebowitz, K. W. Peters, R. A. Frizzell, and J. P. Johnson The Epithelial Sodium Channel (ENaC) Traffics to Apical Membrane in Lipid Rafts in Mouse Cortical Collecting Duct Cells J. Biol. Chem., December 28, 2007; 282(52): 37402 - 37411. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Martel, D. Michael, G. Fejes-Toth, and A. Naray-Fejes-Toth Melanophilin, a novel aldosterone-induced gene in mouse cortical collecting duct cells Am J Physiol Renal Physiol, September 1, 2007; 293(3): F904 - F913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Pochynyuk, A. Staruschenko, V. Bugaj, L. LaGrange, and J. D. Stockand Quantifying RhoA Facilitated Trafficking of the Epithelial Na+ Channel toward the Plasma Membrane with Total Internal Reflection Fluorescence-Fluorescence Recovery after Photobleaching J. Biol. Chem., May 11, 2007; 282(19): 14576 - 14585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Snyder Minireview: Regulation of Epithelial Na+ Channel Trafficking Endocrinology, December 1, 2005; 146(12): 5079 - 5085. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Butterworth, R. A. Frizzell, J. P. Johnson, K. W. Peters, and R. S. Edinger PKA-dependent ENaC trafficking requires the SNARE-binding protein complexin Am J Physiol Renal Physiol, November 1, 2005; 289(5): F969 - F977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Li, Q. Yang, E. A. Alexander, and J. H. Schwartz Syntaxin 1A has a specific binding site in the H3 domain that is critical for targeting of H+-ATPase to apical membrane of renal epithelial cells Am J Physiol Cell Physiol, September 1, 2005; 289(3): C665 - C672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Caldwell, R. C. Boucher, and M. J. Stutts Neutrophil elastase activates near-silent epithelial Na+ channels and increases airway epithelial Na+ transport Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L813 - L819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-J. Sun, W.-Z. Zeng, and C.-L. Huang Inhibition of ROMK potassium channel by syntaxin 1A Am J Physiol Renal Physiol, February 1, 2005; 288(2): F284 - F289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. K. Berdiev, B. Jovov, W. C. Tucker, A. P. Naren, C. M. Fuller, E. R. Chapman, and D. J. Benos ENaC subunit-subunit interactions and inhibition by syntaxin 1A Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1100 - F1106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Condliffe, H. Zhang, and R. A. Frizzell Syntaxin 1A Regulates ENaC Channel Activity J. Biol. Chem., March 12, 2004; 279(11): 10085 - 10092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Biasio, T. Chang, C. J. McIntosh, and F. J. McDonald Identification of Murr1 as a Regulator of the Human {delta} Epithelial Sodium Channel J. Biol. Chem., February 13, 2004; 279(7): 5429 - 5434. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Wang, S. L. Deken, T. L. Whitworth, and M. W. Quick Syntaxin 1A Inhibits GABA Flux, Efflux, and Exchange Mediated by the Rat Brain GABA Transporter GAT1 Mol. Pharmacol., October 1, 2003; 64(4): 905 - 913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. K. Berdiev, J. Xia, L. A. McLean, J. M. Markert, G. Y. Gillespie, T. B. Mapstone, A. P. Naren, B. Jovov, J. K. Bubien, H.-L. Ji, et al. Acid-sensing Ion Channels in Malignant Gliomas J. Biol. Chem., April 18, 2003; 278(17): 15023 - 15034. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Condliffe, M. D. Carattino, R. A. Frizzell, and H. Zhang Syntaxin 1A Regulates ENaC via Domain-specific Interactions J. Biol. Chem., April 4, 2003; 278(15): 12796 - 12804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sterling, D.-H. Lin, Y. Wei, and W.-H. Wang Tetanus toxin abolishes exocytosis of ROMK1 induced by inhibition of protein tyrosine kinase Am J Physiol Renal Physiol, March 1, 2003; 284(3): F510 - F517. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Cormet-Boyaka, A. Di, S. Y. Chang, A. P. Naren, A. Tousson, D. J. Nelson, and K. L. Kirk CFTR chloride channels are regulated by a SNAP-23/syntaxin 1A complex PNAS, September 17, 2002; 99(19): 12477 - 12482. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Bonny and B. C. Rossier Disturbances of Na/K Balance: Pseudohypoaldosteronism Revisited J. Am. Soc. Nephrol., September 1, 2002; 13(9): 2399 - 2414. [Full Text] [PDF]
|
|
|
|
|
|
J. Ji, S. Tsuk, A. M. F. Salapatek, X. Huang, D. Chikvashvili, E. A. Pasyk, Y. Kang, L. Sheu, R. Tsushima, N. Diamant, et al. The 25-kDa Synaptosome-associated Protein (SNAP-25) Binds and Inhibits Delayed Rectifier Potassium Channels in Secretory Cells J. Biol. Chem., May 31, 2002; 277(23): 20195 - 20204. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Humphrey, M. M. Sedensky, and P. G. Morgan Understanding anesthesia: making genetic sense of the absence of senses Hum. Mol. Genet., May 15, 2002; 11(10): 1241 - 1249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Quick Substrates regulate gamma -aminobutyric acid transporters in a syntaxin 1A-dependent manner PNAS, April 16, 2002; 99(8): 5686 - 5691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Snyder The Epithelial Na+ Channel: Cell Surface Insertion and Retrieval in Na+ Homeostasis and Hypertension Endocr. Rev., April 1, 2002; 23(2): 258 - 275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Hanwell, T. Ishikawa, R. Saleki, and D. Rotin Trafficking and Cell Surface Stability of the Epithelial Na+ Channel Expressed in Epithelial Madin-Darby Canine Kidney Cells J. Biol. Chem., March 15, 2002; 277(12): 9772 - 9779. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Y. Chang, A. Di, A. P. Naren, H. C. Palfrey, K. L. Kirk, and D. J. Nelson Mechanisms of CFTR regulation by syntaxin 1A and PKA J. Cell Sci., February 15, 2002; 115(4): 783 - 791. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Geelen, B. Leyman, H. Batoko, G.-P. Di Sansebastiano, I. Moore, and M. R. Blatt The Abscisic Acid-Related SNARE Homolog NtSyr1 Contributes to Secretion and Growth: Evidence from Competition with Its Cytosolic Domain PLANT CELL, February 1, 2002; 14(2): 387 - 406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Volk, P. M. Snyder, and J. B. Stokes Regulation of Epithelial Sodium Channel Activity through a Region of the Carboxyl Terminus of the alpha -Subunit. EVIDENCE FOR INTRACELLULAR KINASE-MEDIATED REACTIONS J. Biol. Chem., November 16, 2001; 276(47): 43887 - 43893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Rotin, V. Kanelis, and L. Schild Trafficking and cell surface stability of ENaC Am J Physiol Renal Physiol, September 1, 2001; 281(3): F391 - F399. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Fili, I. Michaelevski, Y. Bledi, D. Chikvashvili, D. Singer-Lahat, H. Boshwitz, M. Linial, and I. Lotan Direct Interaction of a Brain Voltage-Gated K+ Channel with Syntaxin 1A: Functional Impact on Channel Gating J. Neurosci., March 15, 2001; 21(6): 1964 - 1974. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Bezprozvanny and A. Maximov PDZ domains: More than just a glue PNAS, January 30, 2001; 98(3): 787 - 789. [Full Text] [PDF]
|
|
|
|
|
|
I. Bezprozvanny, P. Zhong, R. H. Scheller, and R. W. Tsien Molecular determinants of the functional interaction between syntaxin and N-type Ca2+ channel gating PNAS, November 16, 2000; (2000) 220389697. [Abstract] [Full Text]
|
|
|
|
|
|
H.-L. Ji, M. L. Chalfant, B. Jovov, J. P. Lockhart, S. B. Parker, C. M. Fuller, B. A. Stanton, and D. J. Benos The Cytosolic Termini of the beta - and gamma -ENaC Subunits Are Involved in the Functional Interactions between Cystic Fibrosis Transmembrane Conductance Regulator and Epithelial Sodium Channel J. Biol. Chem., September 1, 2000; 275(36): 27947 - 27956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Geerlings, E. Nunez, B. Lopez-Corcuera, and C. Aragon Calcium- and Syntaxin 1-mediated Trafficking of the Neuronal Glycine Transporter GLYT2 J. Biol. Chem., May 11, 2001; 276(20): 17584 - 17590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Hanwell, T. Ishikawa, R. Saleki, and D. Rotin Trafficking and Cell Surface Stability of the Epithelial Na+ Channel Expressed in Epithelial Madin-Darby Canine Kidney Cells J. Biol. Chem., March 15, 2002; 277(12): 9772 - 9779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Bezprozvanny, P. Zhong, R. H. Scheller, and R. W. Tsien Molecular determinants of the functional interaction between syntaxin and N-type Ca2+ channel gating PNAS, December 5, 2000; 97(25): 13943 - 13948. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
