HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Michelson, R. J. Articles by Huggenvik, J. I. Search for Related Content PubMed PubMed Citation Articles by Michelson, R. J. Articles by Huggenvik, J. I. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 43, 30510-30519, October 22, 1999

Nuclear DEAF-1-related (NUDR) Protein Contains a Novel DNA Binding Domain and Represses Transcription of the Heterogeneous Nuclear Ribonucleoprotein A2/B1 Promoter

Rhett J. Michelson, Michael W. Collard, Amy J. Ziemba, Jim Persinger^§, Blaine Bartholomew^§, and Jodi I. Huggenvik

From the  Department of Physiology and ^§ Department of Medical Biochemistry, Southern Illinois University School of Medicine, Carbondale, Illinois 62901-6523

Nuclear DEAF-1-related (NUDR) protein is a novel transcriptional regulator with sequence similarity to developmental and oncogenic proteins. NUDR protein deletions were used to localize the DNA binding domain between amino acids 167 and 368, and site-specific DNA photocross-linking indicated at least two sites of protein-DNA contact within this domain. The DNA binding domain contains a proline-rich region and a region with similarity to a Myc-type helix-loop-helix domain but does not include the zinc finger motif at the C terminus. Deoxyribonuclease I protection assays confirmed the presence of multiple NUDR binding motifs (TTC(C/G)G) in the heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) promoter and also in the 5'-untranslated region (UTR) of hNUDR cDNA. NUDR produced a 65-70% repression of the hnRNP A2/B1 promoter activity, and NUDR binding motifs in the 5'-UTR were found to mediate this repression. NUDR-dependent repression was also observed when the 5'-UTR of NUDR was placed onto a heterologous thymidine kinase promoter in an analogous 5'-UTR position but not when placed upstream of transcription initiation. These results suggest that NUDR may regulate the in vivo expression of hnRNP A2/B1 and NUDR genes and imply that inactivation of NUDR could contribute to the overexpression of hnRNP A2/B1 observed in some human cancers.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				D. E. Reed, X. M. Huang, J. A. Wohlschlegel, M. S. Levine, and K. Senger DEAF-1 regulates immunity gene expression in Drosophila PNAS, June 17, 2008; 105(24): 8351 - 8356. [Abstract] [Full Text] [PDF]

				M. Czesak, S. Lemonde, E. A. Peterson, A. Rogaeva, and P. R. Albert Cell-Specific Repressor or Enhancer Activities of Deaf-1 at a Serotonin 1A Receptor Gene Polymorphism J. Neurosci., February 8, 2006; 26(6): 1864 - 1871. [Abstract] [Full Text] [PDF]

				P. R. Albert and S. Lemonde 5-HT1A Receptors, Gene Repression, and Depression: Guilt by Association Neuroscientist, December 1, 2004; 10(6): 575 - 593. [Abstract] [PDF]

				S. Lemonde, G. Turecki, D. Bakish, L. Du, P. D. Hrdina, C. D. Bown, A. Sequeira, N. Kushwaha, S. J. Morris, A. Basak, et al. Impaired Repression at a 5-Hydroxytryptamine 1A Receptor Gene Polymorphism Associated with Major Depression and Suicide J. Neurosci., September 24, 2003; 23(25): 8788 - 8799. [Abstract] [Full Text] [PDF]

				P. L. Surdo, M. J. Bottomley, M. Sattler, and K. Scheffzek Crystal Structure and Nuclear Magnetic Resonance Analyses of the SAND Domain from Glucocorticoid Modulatory Element Binding Protein-1 Reveals Deoxyribonucleic Acid and Zinc Binding Regions Mol. Endocrinol., July 1, 2003; 17(7): 1283 - 1295. [Abstract] [Full Text] [PDF]

				M. Garayoa, Y.-G. Man, A. Martinez, F. Cuttitta, and J. L. Mulshine Downregulation of hnRNP A2/B1 Expression in Tumor Cells under Prolonged Hypoxia Am. J. Respir. Cell Mol. Biol., January 1, 2003; 28(1): 80 - 85. [Abstract] [Full Text] [PDF]

				E. Sueoka, N. Sueoka, Y. Goto, S. Matsuyama, H. Nishimura, M. Sato, S. Fujimura, H. Chiba, and H. Fujiki Heterogeneous Nuclear Ribonucleoprotein B1 as Early Cancer Biomarker for Occult Cancer of Human Lungs and Bronchial Dysplasia Cancer Res., March 1, 2001; 61(5): 1896 - 1902. [Abstract] [Full Text]