HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stradal, T. B. Articles by Gimona, M. Search for Related Content PubMed PubMed Citation Articles by Stradal, T. B. Articles by Gimona, M. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 44, 31593-31596, October 29, 1999

Ca²⁺-dependent Association of S100A6 (Calcyclin) with the Plasma Membrane and the Nuclear Envelope

From the Department of Cell Biology, Institute of Molecular Biology, Austrian Academy of Sciences, Billrothstrasse 11, A-5020 Salzburg, Austria

Expression of S100A6 (Calcyclin), a member of the S100 family and of Zn²⁺-binding proteins is elevated in a number of malignant tumors. In vitro the protein associates with several actin-binding proteins and annexins in a Ca²⁺-dependent manner. We have now studied the subcellular localization of S100A6 using a new, specific monoclonal antibody. Immunofluorescence microscopy of unfixed, ultrathin, frozen sections demonstrated a dual localization of S100A6 at the nuclear envelope and the plasma membrane of porcine smooth muscle only in the presence of Ca²⁺. The same localization was found by immunofluorescence and immunogold electron microscopy as well as by confocal laser scanning microscopy with cultured, fixed, human CaKi-2 and porcine ST interphase cells. Upon cell division, however, S100A6 was found exclusively in the cytoplasm. Cell fractionation studies showed that S100A6 was present in the microsomal fraction in the presence of Ca²⁺ and was released from this fraction by the addition of EGTA/EDTA but not by Triton X-100. The data demonstrate that S100A6 is localized both at the plasma membrane and the nuclear envelope in vivo and suggest a Ca²⁺-dependent interaction with annexins or other components of the nuclear envelope.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				L. M. Orre, M. Pernemalm, J. Lengqvist, R. Lewensohn, and J. Lehtio Up-regulation, Modification, and Translocation of S100A6 Induced by Exposure to Ionizing Radiation Revealed by Proteomics Profiling Mol. Cell. Proteomics, December 1, 2007; 6(12): 2122 - 2131. [Abstract] [Full Text] [PDF]

				L. Farnaes and H. J. Ditzel Dissecting the Cellular Functions of Annexin XI Using Recombinant Human Annexin XI-specific Autoantibodies Cloned by Phage Display J. Biol. Chem., August 29, 2003; 278(35): 33120 - 33126. [Abstract] [Full Text] [PDF]

				A. Tomas and S. E. Moss Calcium- and Cell Cycle-dependent Association of Annexin 11 with the Nuclear Envelope J. Biol. Chem., May 23, 2003; 278(22): 20210 - 20216. [Abstract] [Full Text] [PDF]

				P. J. Adam, R. Boyd, K. L. Tyson, G. C. Fletcher, A. Stamps, L. Hudson, H. R. Poyser, N. Redpath, M. Griffiths, G. Steers, et al. Comprehensive Proteomic Analysis of Breast Cancer Cell Membranes Reveals Unique Proteins with Potential Roles in Clinical Cancer J. Biol. Chem., February 14, 2003; 278(8): 6482 - 6489. [Abstract] [Full Text] [PDF]

				N. Courtois-Coutry, C. Le Moellic, S. Boulkroun, M. Fay, F. Cluzeaud, B. Escoubet, N. Farman, and M. Blot-Chabaud Calcyclin Is an Early Vasopressin-induced Gene in the Renal Collecting Duct. ROLE IN THE LONG TERM REGULATION OF ION TRANSPORT J. Biol. Chem., July 5, 2002; 277(28): 25728 - 25734. [Abstract] [Full Text] [PDF]

				A. Filipek, B. Jastrzebska, M. Nowotny, K. Kwiatkowska, M. Hetman, L. Surmacz, E. Wyroba, and J. Kuznicki Ca2+-dependent Translocation of the Calcyclin-binding Protein in Neurons and Neuroblastoma NB-2a Cells J. Biol. Chem., May 31, 2002; 277(23): 21103 - 21109. [Abstract] [Full Text] [PDF]

				H.-L. Hsieh, B. W. Schafer, J. A. Cox, and C. W. Heizmann S100A13 and S100A6 exhibit distinct translocation pathways in endothelial cells J. Cell Sci., January 8, 2002; 115(15): 3149 - 3158. [Abstract] [Full Text] [PDF]

				T. B. Stradal, H. Troxler, C. W. Heizmann, and M. Gimona Mapping the Zinc Ligands of S100A2 by Site-directed Mutagenesis J. Biol. Chem., April 28, 2000; 275(18): 13219 - 13227. [Abstract] [Full Text] [PDF]