HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Alberdi, E. Articles by Becerra, S. P. Search for Related Content PubMed PubMed Citation Articles by Alberdi, E. Articles by Becerra, S. P. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 44, 31605-31612, October 29, 1999

Binding of Pigment Epithelium-derived Factor (PEDF) to Retinoblastoma Cells and Cerebellar Granule Neurons
EVIDENCE FOR A PEDF RECEPTOR

From the Laboratory of Retinal Cell and Molecular Biology, NEI, National Institutes of Health, Bethesda, Maryland 20892

Pigment epithelium-derived factor (PEDF) has neuronal differentiation and survival activity on retinoblastoma and cerebellar granule (CG) cells. Here, we investigated the presence of PEDF receptors on retinoblastoma Y-79 and CG cells. PEDF radiolabeled with ^l25I remained biologically active and was used for radioligand binding analysis. The binding was saturable and specific to a single class of receptors on both cells and with similar affinities (K_d = 1.7-3.6 nM, B_max = 0.5-2.7 × 10⁵ sites/Y-79 cell; and K_d = 3.2 nM, B_max = 1.1 × 10³ sites/CG cell). A polyclonal antiserum to PEDF, previously shown to block the PEDF neurotrophic activity, prevented the ¹²⁵I-PEDF binding. We designed two peptides from a region previously shown to confer the neurotrophic property to human PEDF, synthetic peptides 34-mer (positions 44-77) and 44-mer (positions 78-121). Only peptide 44-mer competed for the binding to Y-79 cell receptors (EC₅₀ = 5 nM) and exhibited neuronal differentiating activity. PEDF affinity column chromatography of membrane proteins from both cell types revealed a PEDF-binding protein of ~80 kDa. These results are the first demonstration of a PEDF-binding protein with characteristics of a PEDF receptor and suggest that the region comprising amino acid positions 78-121 of PEDF might be involved in ligand-receptor interactions.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				A. Bernard, J. Gao-Li, C.-A. Franco, T. Bouceba, A. Huet, and Z. Li Laminin Receptor Involvement in the Anti-angiogenic Activity of Pigment Epithelium-derived Factor J. Biol. Chem., April 17, 2009; 284(16): 10480 - 10490. [Abstract] [Full Text] [PDF]

				S. P. Becerra, L. A. Perez-Mediavilla, J. E. Weldon, S. Locatelli-Hoops, P. Senanayake, L. Notari, V. Notario, and J. G. Hollyfield Pigment Epithelium-derived Factor Binds to Hyaluronan: MAPPING OF A HYALURONAN BINDING SITE J. Biol. Chem., November 28, 2008; 283(48): 33310 - 33320. [Abstract] [Full Text] [PDF]

				L. Notari, V. Baladron, J. D. Aroca-Aguilar, N. Balko, R. Heredia, C. Meyer, P. M. Notario, S. Saravanamuthu, M.-L. Nueda, F. Sanchez-Sanchez, et al. Identification of a Lipase-linked Cell Membrane Receptor for Pigment Epithelium-derived Factor J. Biol. Chem., December 8, 2006; 281(49): 38022 - 38037. [Abstract] [Full Text] [PDF]

				E. T.H. Ek, C. R. Dass, and P. F.M. Choong Pigment epithelium-derived factor: a multimodal tumor inhibitor. Mol. Cancer Ther., July 1, 2006; 5(7): 1641 - 1646. [Abstract] [Full Text] [PDF]

				D. M. Sidle, J. Maddalozzo, J. D. Meier, M. Cornwell, V. Stellmach, and S. E. Crawford Altered Pigment Epithelium-Derived Factor and Vascular Endothelial Growth Factor Levels in Lymphangioma Pathogenesis and Clinical Recurrence Arch Otolaryngol Head Neck Surg, November 1, 2005; 131(11): 990 - 995. [Abstract] [Full Text] [PDF]

				S. Filleur, K. Volz, T. Nelius, Y. Mirochnik, H. Huang, T. A. Zaichuk, M. S. Aymerich, S. P. Becerra, R. Yap, D. Veliceasa, et al. Two Functional Epitopes of Pigment Epithelial-Derived Factor Block Angiogenesis and Induce Differentiation in Prostate Cancer Cancer Res., June 15, 2005; 65(12): 5144 - 5152. [Abstract] [Full Text] [PDF]

				S. Kanda, Y. Mochizuki, T. Nakamura, Y. Miyata, T. Matsuyama, and H. Kanetake Pigment epithelium-derived factor inhibits fibroblast-growth-factor-2-induced capillary morphogenesis of endothelial cells through Fyn J. Cell Sci., March 1, 2005; 118(5): 961 - 970. [Abstract] [Full Text] [PDF]

				G. Maik-Rachline, S. Shaltiel, and R. Seger Extracellular phosphorylation converts pigment epithelium-derived factor from a neurotrophic to an antiangiogenic factor Blood, January 15, 2005; 105(2): 670 - 678. [Abstract] [Full Text] [PDF]

				M. Al-Ayyoubi, P. G. W. Gettins, and K. Volz Crystal Structure of Human Maspin, a Serpin with Antitumor Properties: REACTIVE CENTER LOOP OF MASPIN IS EXPOSED BUT CONSTRAINED J. Biol. Chem., December 31, 2004; 279(53): 55540 - 55544. [Abstract] [Full Text] [PDF]

				R. S. Apte, R. A. Barreiro, E. Duh, O. Volpert, and T. A. Ferguson Stimulation of Neovascularization by the Anti-angiogenic Factor PEDF Invest. Ophthalmol. Vis. Sci., December 1, 2004; 45(12): 4491 - 4497. [Abstract] [Full Text] [PDF]

				M. Garcia, N. I. Fernandez-Garcia, V. Rivas, M. Carretero, M. J. Escamez, A. Gonzalez-Martin, E. E. Medrano, O. Volpert, J. L. Jorcano, B. Jimenez, et al. Inhibition of Xenografted Human Melanoma Growth and Prevention of Metastasis Development by Dual Antiangiogenic/Antitumor Activities of Pigment Epithelium-Derived Factor Cancer Res., August 15, 2004; 64(16): 5632 - 5642. [Abstract] [Full Text] [PDF]

				S. Halin, P. Wikstrom, S. H. Rudolfsson, P. Stattin, J. A. Doll, S. E. Crawford, and A. Bergh Decreased Pigment Epithelium-Derived Factor Is Associated with Metastatic Phenotype in Human and Rat Prostate Tumors Cancer Res., August 15, 2004; 64(16): 5664 - 5671. [Abstract] [Full Text] [PDF]

				H. Liu, J.-G. Ren, W. L. Cooper, C. E. Hawkins, M. R. Cowan, and P. Y. Tong Identification of the antivasopermeability effect of pigment epithelium-derived factor and its active site PNAS, April 27, 2004; 101(17): 6605 - 6610. [Abstract] [Full Text] [PDF]

				C. Ngamkitidechakul, D. J. Warejcka, J. M. Burke, W. J. O'Brien, and S. S. Twining Sufficiency of the Reactive Site Loop of Maspin for Induction of Cell-Matrix Adhesion and Inhibition of Cell Invasion: CONVERSION OF OVALBUMIN TO A MASPIN-LIKE MOLECULE J. Biol. Chem., August 22, 2003; 278(34): 31796 - 31806. [Abstract] [Full Text] [PDF]

				C. Meyer, L. Notari, and S. P. Becerra Mapping the Type I Collagen-binding Site on Pigment Epithelium-derived Factor. IMPLICATIONS FOR ITS ANTIANGIOGENIC ACTIVITY J. Biol. Chem., November 15, 2002; 277(47): 45400 - 45407. [Abstract] [Full Text] [PDF]

				M. M. Bilak, S. P. Becerra, A. M. Vincent, B. H. Moss, M. S. Aymerich, and R. W. Kuncl Identification of the Neuroprotective Molecular Region of Pigment Epithelium-Derived Factor and Its Binding Sites on Motor Neurons J. Neurosci., November 1, 2002; 22(21): 9378 - 9386. [Abstract] [Full Text] [PDF]

				N. Ogata, M. Wada, T. Otsuji, N. Jo, J. Tombran-Tink, and M. Matsumura Expression of Pigment Epithelium-Derived Factor in Normal Adult Rat Eye and Experimental Choroidal Neovascularization Invest. Ophthalmol. Vis. Sci., April 1, 2002; 43(4): 1168 - 1175. [Abstract] [Full Text] [PDF]

				M. S. Aymerich, E. M. Alberdi, A. Martinez, and S. P. Becerra Evidence for Pigment Epithelium-Derived Factor Receptors in the Neural Retina Invest. Ophthalmol. Vis. Sci., December 1, 2001; 42(13): 3287 - 3293. [Abstract] [Full Text] [PDF]

				J. Spranger, M. Osterhoff, M. Reimann, M. Mohlig, M. Ristow, M. K. Francis, V. Cristofalo, H.-P. Hammes, G. Smith, M. Boulton, et al. Loss of the Antiangiogenic Pigment Epithelium-Derived Factor in Patients With Angiogenic Eye Disease Diabetes, December 1, 2001; 50(12): 2641 - 2645. [Abstract] [Full Text] [PDF]

				M. Simonovic, P. G. W. Gettins, and K. Volz Crystal structure of human PEDF, a potent anti-angiogenic and neurite growth-promoting factor PNAS, September 13, 2001; (2001) 211268598. [Abstract] [Full Text] [PDF]

				W. Cao, J. Tombran-Tink, R. Elias, S. Sezate, D. Mrazek, and J. F. McGinnis In Vivo Protection of Photoreceptors from Light Damage by Pigment Epithelium-Derived Factor Invest. Ophthalmol. Vis. Sci., June 1, 2001; 42(7): 1646 - 1652. [Abstract] [Full Text]

				V. Stellmach, S. E. Crawford, W. Zhou, and N. Bouck Prevention of ischemia-induced retinopathy by the natural ocular antiangiogenic agent pigment epithelium-derived factor PNAS, January 23, 2001; (2001) 31252398. [Abstract] [Full Text]

				V. Stellmach, S. E. Crawford, W. Zhou, and N. Bouck Prevention of ischemia-induced retinopathy by the natural ocular antiangiogenic agent pigment epithelium-derived factor PNAS, February 27, 2001; 98(5): 2593 - 2597. [Abstract] [Full Text] [PDF]

				M. Simonovic, P. G. W. Gettins, and K. Volz Crystal structure of human PEDF, a potent anti-angiogenic and neurite growth-promoting factor PNAS, September 25, 2001; 98(20): 11131 - 11135. [Abstract] [Full Text] [PDF]