| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 45, 31863-31867, November 5, 1999
,,,
From the The Kaposi's sarcoma-associated herpesvirus
(KSHV) (also known as human herpesvirus 8) has been implicated in the
pathogenesis of Kaposi's sarcoma and B cell primary effusion
lymphomas. KSHV encodes a G protein-coupled receptor (GPCR) that acts
as an oncogene and constitutively activates two protein kinases, c-Jun
amino-terminal kinase (JNK)/stress-activated protein kinase (SAPK) and
p38 mitogen-activated protein kinase. It also induces the production of
vascular endothelial growth factor. These processes are believed to be
important in KSHV-GPCR-related oncogenesis. We have characterized the
signaling pathways mediated by KSHV-GPCR in a reconstituted 293T cell
model in which the related adhesion focal tyrosine kinase (RAFTK) was ectopically expressed. RAFTK has been shown to play an important role
in growth factor signaling in endothelium and in B cell antigen receptor signaling in B lymphocytes. KSHV-GPCR induced the tyrosine phosphorylation of RAFTK. Expression of wild-type RAFTK enhanced GPCR-mediated JNK/SAPK activation, whereas dominant-negative mutant constructs of RAFTK, such as K457A (which lacks kinase activity) and
Y402F (a Src-binding mutant), inhibited KSHV-GPCR-mediated activation
of JNK/SAPK. RAFTK also mediated the KSHV-GPCR-induced activation of
Lyn, a Src family kinase. However, RAFTK did not mediate the activation
of p38 mitogen-activated protein kinase induced by KSHV-GPCR. Human
interferon Division of Experimental Medicine and
Hematology/Oncology, Robert Mapplethorpe Laboratory, Beth Israel
Deaconess Medical Center, Harvard Medical School, Boston,
Massachusetts 02115 and the ¶ Laboratory of Viral Oncogenesis,
Division of Hematology-Oncology, Cornell University Medical College,
New York, New York 10021
-inducible protein-10, which is known to inhibit
KSHV-GPCR activity, was found to reduce RAFTK phosphorylation and
JNK/SAPK activation. These results suggest that in cells expressing
RAFTK/proline-rich tyrosine kinase 2, such as endothelial and B cells,
RAFTK can act to enhance KSHV-GPCR-mediated downstream signaling to
transcriptional regulators such as JNK/SAPK.
This article has been cited by other articles:
|
|
 |
|
  A. Prasad, Z. Qamri, J. Wu, and R. K. Ganju Slit-2/Robo-1 modulates the CXCL12/CXCR4-induced chemotaxis of T cells J. Leukoc. Biol., September 1, 2007; 82(3): 465 - 476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Rosenkilde, R. David, I. Oerlecke, T. Benned-Jensen, U. Geumann, A. G. Beck-Sickinger, and T. W. Schwartz Conformational Constraining of Inactive and Active States of a Seven Transmembrane Receptor by Metal Ion Site Engineering in the Extracellular End of Transmembrane Segment V Mol. Pharmacol., December 1, 2006; 70(6): 1892 - 1901. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Cohen, C. Brodie, and R. Sarid An essential role of ERK signalling in TPA-induced reactivation of Kaposi's sarcoma-associated herpesvirus. J. Gen. Virol., April 1, 2006; 87(Pt 4): 795 - 802. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-G. Guo, S. Pati, M. Sadowska, M. Charurat, and M. Reitz Tumorigenesis by Human Herpesvirus 8 vGPCR Is Accelerated by Human Immuodeficiency Virus Type 1 Tat J. Virol., September 1, 2004; 78(17): 9336 - 9342. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Rosenkilde, K. A. McLean, P. J. Holst, and T. W. Schwartz The CXC Chemokine Receptor Encoded by Herpesvirus saimiri, ECRF3, Shows Ligand-regulated Signaling through Gi, Gq, and G12/13 Proteins but Constitutive Signaling Only through Gi and G12/13 Proteins J. Biol. Chem., July 30, 2004; 279(31): 32524 - 32533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Prasad, A. Z. Fernandis, Y. Rao, and R. K. Ganju Slit Protein-mediated Inhibition of CXCR4-induced Chemotactic and Chemoinvasive Signaling Pathways in Breast Cancer Cells J. Biol. Chem., March 5, 2004; 279(10): 9115 - 9124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Liu, G. Sandford, G. Fei, and J. Nicholas G{alpha} Protein Selectivity Determinant Specified by a Viral Chemokine Receptor-Conserved Region in the C Tail of the Human Herpesvirus 8 G Protein-Coupled Receptor J. Virol., March 1, 2004; 78(5): 2460 - 2471. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. SODHI, S. MONTANER, and J. S. GUTKIND Does dysregulated expression of a deregulated viral GPCR trigger Kaposi's sarcomagenesis? FASEB J, March 1, 2004; 18(3): 422 - 427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. E. Miller, D. A. Houtz, C. D. Nelson, P. E. Kolattukudy, and R. J. Lefkowitz G-protein-coupled Receptor (GPCR) Kinase Phosphorylation and {beta}-Arrestin Recruitment Regulate the Constitutive Signaling Activity of the Human Cytomegalovirus US28 GPCR J. Biol. Chem., June 6, 2003; 278(24): 21663 - 21671. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Dourmishev, A. L. Dourmishev, D. Palmeri, R. A. Schwartz, and D. M. Lukac Molecular Genetics of Kaposi's Sarcoma-Associated Herpesvirus (Human Herpesvirus 8) Epidemiology and Pathogenesis Microbiol. Mol. Biol. Rev., June 1, 2003; 67(2): 175 - 212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-G. Guo, M. Sadowska, W. Reid, E. Tschachler, G. Hayward, and M. Reitz Kaposi's Sarcoma-Like Tumors in a Human Herpesvirus 8 ORF74 Transgenic Mouse J. Virol., February 15, 2003; 77(4): 2631 - 2639. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Estep, M. K. Axthelm, and S. W. Wong A G Protein-Coupled Receptor Encoded by Rhesus Rhadinovirus Is Similar to ORF74 of Kaposi's Sarcoma-Associated Herpesvirus J. Virol., February 1, 2003; 77(3): 1738 - 1746. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. G. Polson, D. Wang, J. DeRisi, and D. Ganem Modulation of Host Gene Expression by the Constitutively Active G Protein-coupled Receptor of Kaposi's Sarcoma-associated Herpesvirus Cancer Res., August 1, 2002; 62(15): 4525 - 4530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. C. Xu, R. F. Wu, Y. Gu, Y.-S. Yang, M.-C. Yang, F. E. Nwariaku, and L. S. Terada Involvement of TRAF4 in Oxidative Activation of c-Jun N-terminal Kinase J. Biol. Chem., July 26, 2002; 277(31): 28051 - 28057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. W. Shepard, M. Yang, P. Xie, D. D. Browning, T. Voyno-Yasenetskaya, T. Kozasa, and R. D. Ye Constitutive Activation of NF-kappa B and Secretion of Interleukin-8 Induced by the G Protein-coupled Receptor of Kaposi's Sarcoma-associated Herpesvirus Involve Galpha 13 and RhoA J. Biol. Chem., November 30, 2001; 276(49): 45979 - 45987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Masood, J. Cai, A. Tulpule, T. Zheng, A. Hamilton, S. Sharma, B. M. Espina, D. L. Smith, and P. S. Gill Interleukin 8 Is an Autocrine Growth Factor and a Surrogate Marker for Kaposi's Sarcoma Clin. Cancer Res., September 1, 2001; 7(9): 2693 - 2702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Montaner, A. Sodhi, S. Pece, E. A. Mesri, and J. S. Gutkind The Kaposi's Sarcoma-associated Herpesvirus G Protein-coupled Receptor Promotes Endothelial Cell Survival through the Activation of Akt/Protein Kinase B Cancer Res., March 1, 2001; 61(6): 2641 - 2648. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
