| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 45, 31947-31954, November 5, 1999
From the Department of Microbiology and Molecular Genetics,
University of Medicine and Dentistry of New Jersey, New Jersey Medical
School, Newark, New Jersey 07103-2714
Eukaryotic transcriptional regulatory signals,
defined as core and activator promoter elements, have yet to be
identified in the earliest diverging group of eukaryotes, the primitive
protozoans, which include the Trypanosomatidae family of parasites. The
divergence within this family is highlighted by the apparent absence of
the "universal" transcription factor TATA-binding protein. To
understand gene expression in these protists, we have investigated
spliced leader RNA gene transcription. The RNA product of this gene
provides an m7G cap and a 39-nucleotide leader
sequence to all cellular mRNAs via a trans-splicing reaction.
Regulation of spliced leader RNA synthesis is controlled by a
tripartite promoter located exclusively upstream from the transcription
start site. Proteins PBP-1 and PBP-2 bind to two of the three promoter
elements in the trypanosomatid Leptomonas seymouri. They
represent the first trypanosome transcription factors with typical
double-stranded DNA binding site recognition. These proteins ensure
efficient transcription. However, accurate initiation is determined an
initiator element with a a loose consensus of CYAC/AYR (+1), which
differs from that found in metazoan initiator elements as well as from
that identified in one of the earliest diverging protozoans,
Trichomonas vaginalis. Trypanosomes may utilize initiator
element-protein interactions, and not TATA sequence-TATA-binding protein interactions, to direct proper transcription initiation by RNA
polymerase II.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. H. Lee, T. N. Nguyen, B. Schimanski, and A. Gunzl Spliced Leader RNA Gene Transcription in Trypanosoma brucei Requires Transcription Factor TFIIH Eukaryot. Cell, April 1, 2007; 6(4): 641 - 649. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Schimanski, J. Brandenburg, T. N. Nguyen, M. J. Caimano, and A. Gunzl A TFIIB-like protein is indispensable for spliced leader RNA gene transcription in Trypanosoma brucei Nucleic Acids Res., March 22, 2006; 34(6): 1676 - 1684. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Schimanski, T. N. Nguyen, and A. Gunzl Characterization of a Multisubunit Transcription Factor Complex Essential for Spliced-Leader RNA Gene Transcription in Trypanosoma brucei Mol. Cell. Biol., August 15, 2005; 25(16): 7303 - 7313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Das, Q. Zhang, J. B. Palenchar, B. Chatterjee, G. A. M. Cross, and V. Bellofatto Trypanosomal TBP Functions with the Multisubunit Transcription Factor tSNAP To Direct Spliced-Leader RNA Gene Expression Mol. Cell. Biol., August 15, 2005; 25(16): 7314 - 7322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Martinez-Calvillo, D. Nguyen, K. Stuart, and P. J. Myler Transcription Initiation and Termination on Leishmania major Chromosome 3 Eukaryot. Cell, April 1, 2004; 3(2): 506 - 517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Schimanski, G. Laufer, L. Gontcharova, and A. Gunzl The Trypanosoma brucei spliced leader RNA and rRNA gene promoters have interchangeable TbSNAP50-binding elements Nucleic Acids Res., February 2, 2004; 32(2): 700 - 709. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Landfear Trypanosomatid transcription factors: Waiting for Godot PNAS, January 7, 2003; 100(1): 7 - 9. [Full Text] [PDF]
|
|
|
|
|
|
A. Das and V. Bellofatto From the Cover: RNA polymerase II-dependent transcription in trypanosomes is associated with a SNAP complex-like transcription factor PNAS, January 7, 2003; 100(1): 80 - 85. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Guillebault, S. Sasorith, E. Derelle, J.-M. Wurtz, J.-C. Lozano, S. Bingham, L. Tora, and H. Moreau A New Class of Transcription Initiation Factors, Intermediate between TATA Box-binding Proteins (TBPs) and TBP-like Factors (TLFs), Is Present in the Marine Unicellular Organism, the Dinoflagellate Crypthecodinium cohnii J. Biol. Chem., October 18, 2002; 277(43): 40881 - 40886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Gilinger and V. Bellofatto Trypanosome spliced leader RNA genes contain the first identified RNA polymerase II gene promoter in these organisms Nucleic Acids Res., April 1, 2001; 29(7): 1556 - 1564. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
