| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 45, 32071-32078, November 5, 1999
From the Institute of Medical Virology, University of Zürich,
CH-8028 Zürich, Switzerland
Mx proteins are large GTPases, which play a
pivotal role in the interferon type I-mediated response against viral
infections. The human MxA inhibits the replication of several RNA
viruses and is organized in oligomeric structures. Using two different experimental approaches, the mammalian two-hybrid system and an interaction dependent nuclear translocation approach, three domains in
the carboxyl-terminal moiety were identified that are involved in the
oligomerization of MxA. The first consists of a carboxyl-terminal amphipathic helix (LZ1), which binds to a more proximal part of the
same molecule. This intramolecular backfolding is a prerequisite for
the formation of an intermolecular complex. This intermolecular interaction is mediated by two domains, a poorly defined region generated by the intramolecular interaction and a domain located between amino acids 363 and 415. Co-expression of wild-type MxA with
various mutant fragments thereof revealed that the presence of the
carboxyl-terminal region comprising the amphipathic helices LZ1 and LZ2
is necessary and sufficient to exert a dominant negative effect. This
finding suggests that the functional interference of the
carboxyl-terminal region is due to competition for binding of an as yet
unidentified cellular or viral target molecules.
This article has been cited by other articles:
|
|
 |
|
  C.-R. Chang and C. Blackstone Cyclic AMP-dependent Protein Kinase Phosphorylation of Drp1 Regulates Its GTPase Activity and Mitochondrial Morphology J. Biol. Chem., July 27, 2007; 282(30): 21583 - 21587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Lussier, S. Cayouette, P. K. Lepage, C. L. Bernier, N. Francoeur, M. St-Hilaire, M. Pinard, and G. Boulay MxA, a Member of the Dynamin Superfamily, Interacts with the Ankyrin-like Repeat Domain of TRPC J. Biol. Chem., May 13, 2005; 280(19): 19393 - 19400. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-P. Zhu, A. Patterson, J. Stadler, D. P. Seeburg, M. Sheng, and C. Blackstone Intra- and Intermolecular Domain Interactions of the C-terminal GTPase Effector Domain of the Multimeric Dynamin-like GTPase Drp1 J. Biol. Chem., August 20, 2004; 279(34): 35967 - 35974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Turan, M. Mibayashi, K. Sugiyama, S. Saito, A. Numajiri, and K. Nagata Nuclear MxA proteins form a complex with influenza virus NP and inhibit the transcription of the engineered influenza virus genome Nucleic Acids Res., January 29, 2004; 32(2): 643 - 652. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-P. Zhu, A. Patterson, B. Lavoie, J. Stadler, M. Shoeb, R. Patel, and C. Blackstone Cellular Localization, Oligomerization, and Membrane Association of the Hereditary Spastic Paraplegia 3A (SPG3A) Protein Atlastin J. Biol. Chem., December 5, 2003; 278(49): 49063 - 49071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Accola, B. Huang, A. Al Masri, and M. A. McNiven The Antiviral Dynamin Family Member, MxA, Tubulates Lipids and Localizes to the Smooth Endoplasmic Reticulum J. Biol. Chem., June 7, 2002; 277(24): 21829 - 21835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Kochs, M. Haener, U. Aebi, and O. Haller Self-assembly of Human MxA GTPase into Highly Ordered Dynamin-like Oligomers J. Biol. Chem., April 12, 2002; 277(16): 14172 - 14176. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-H. Lee and S. M. Vidal Functional Diversity of Mx Proteins: Variations on a Theme of Host Resistance to Infection Genome Res., April 1, 2002; 12(4): 527 - 530. [Full Text] [PDF]
|
|
|
|
 |
|
 G. Kochs, C. Janzen, H. Hohenberg, and O. Haller Antivirally active MxA protein sequesters La Crosse virus nucleocapsid protein into perinuclear complexes PNAS, March 5, 2002; 99(5): 3153 - 3158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Janzen, G. Kochs, and O. Haller A Monomeric GTPase-Negative MxA Mutant with Antiviral Activity J. Virol., September 1, 2000; 74(17): 8202 - 8206. [Abstract] [Full Text]
|
|
|
|
|
|
B. Zhang, Y. Gao, S. Y. Moon, Y. Zhang, and Y. Zheng Oligomerization of Rac1 GTPase Mediated by the Carboxyl-terminal Polybasic Domain J. Biol. Chem., March 16, 2001; 276(12): 8958 - 8967. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
